LOCUS       sequence-465056-       10181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human Delta-37-PPM1H with C-terminal HA 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Wondwossen M Yeshaw , Ayan Adhikari, Claire Y Chiang, Herschel S 
            Dhekne, Paulina S Wawro, Suzanne R Pfeffer
  TITLE     Localization of PPM1H phosphatase tunes Parkinson's disease-linked 
            LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Oct 31;120(44)
REFERENCE   2  (bases 1 to 10181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2023 Oct 31;120(44)"
COMMENT     Sequence Label: Lenti-TO-Delta-37 PPM1H -PACT-HA
FEATURES             Location/Qualifiers
     source          1..10181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             293..349
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             350..748
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    755..1343
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(808..828)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1226..1237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1417..1650
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1722..1843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1759..1778)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1813..1832
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1883..2018
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2051..2067)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2051..2067)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2064..2086)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2075..2091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2099..2105,2106..2123,2124..2129))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2144..2165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2282..2299)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2453..3041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(3212..4003,4004..4072))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3835..3854
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4073..4177)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4245..4263
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4301..4323)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4423..4442
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4636..4658
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4650..4667
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4651..4667
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4682..4908
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4909..5089
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5136..5261
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5754..5987
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6172..6216
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6483..6599
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6632..6650
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6668..6686
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6704..6722
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6740..6758
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6776..6794
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6812..6830
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6848..6866
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6911..6935
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             9133..9159
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        9201..9412
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             9425..9693
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             join(9721..10181,1..283)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 caaagaggaa agagagacac ccaccaccga cagcatgccc cccctgctga agcaggccat
       61 cgagctgttc gacagacagg gcgccgagcc cgccttcctg ttcggcctgg aactgatcat
      121 ctgcggcctc gagaagcagc tgaagtgcga gagcggcgga cccaccgacg ccctggacga
      181 cttcgacctg gacatgctgc ccgccgatgc cctggatgat tttgatctgg atatgctccc
      241 tgctgacgct ctcgatgact tcgatctcga tatgctgcca ggcggaagcg gagctactaa
      301 cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggaccta tggccaagcc
      361 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
      421 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
      481 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
      541 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
      601 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
      661 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
      721 gccctctggt tatgtgtggg agggctaagt cgacaatcaa cctctggatt acaaaatttg
      781 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
      841 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
      901 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
      961 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     1021 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     1081 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     1141 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     1201 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     1261 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     1321 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     1381 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     1441 gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     1501 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     1561 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     1621 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     1681 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     1741 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     1801 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     1861 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     1921 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     1981 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat
     2041 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     2101 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2161 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2221 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2281 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2341 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2401 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2461 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2521 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2581 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2641 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2701 gctgtgtgca cgaacccccc gttcagccca accgctgcgc cttatccggt aactatcgtc
     2761 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2821 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2881 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2941 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3001 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3061 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3121 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3181 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3241 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3301 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3361 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3421 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3481 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3541 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3601 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3661 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3721 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3781 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3841 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3901 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3961 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4021 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4081 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4141 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4201 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4261 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4321 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4381 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     4441 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     4501 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4561 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     4621 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgacgc
     4681 gtgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     4741 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     4801 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     4861 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     4921 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     4981 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5041 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     5101 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     5161 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     5221 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     5281 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     5341 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     5401 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     5461 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     5521 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     5581 agaccaccgc acagcaagcg gccactgatc ttcagacctg gaggaggaga tatgagggac
     5641 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     5701 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     5761 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     5821 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     5881 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     5941 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     6001 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     6061 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     6121 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     6181 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     6241 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     6301 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     6361 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     6421 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     6481 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     6541 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     6601 tcgatactag ttccacccca cagtggttta ctccctatca gtgatagaga acgtatgaag
     6661 agtttactcc ctatcagtga tagagaacgt atgcagactt tactccctat cagtgataga
     6721 gaacgtataa ggagtttact ccctatcagt gatagagaac gtatgaccag tttactccct
     6781 atcagtgata gagaacgtat ctacagttta ctccctatca gtgatagaga acgtatatcc
     6841 agtttactcc ctatcagtga tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct
     6901 ataaaagcag agctcgttta gtgaaccgtc agatcgcctg gagcaattcc acaacacttt
     6961 tgtcttatac caactttccg taccacttcc taccctcgta aatctagaat gcgtttcccc
     7021 tacgggcggc cagagttcct ggggctgtct caggacgagg tggagtgcag cgccgaccac
     7081 atcgcccgcc ccatcctcat cctcaaggag actcggcggc tgccctgggc cactggctac
     7141 gcagaggtta tcaatgccgg gaagagcaca cacaatgaag accaagccag ctgtgaggtg
     7201 ctcactgtga agaagaaggc aggggccgtg acctcaaccc caaacaggaa ctcatccaag
     7261 agacggtcct cccttcccaa tggggaaggg ctgcagctga aggagaactc ggaatccgag
     7321 ggtgtttcct gccactattg gtcgctgttt gacgggcacg cggggtccgg ggccgcggtg
     7381 gtggcgtcac gcctgctgca gcaccacatc acggagcagc tgcaggacat cgtggacatc
     7441 ctgaagaact ccgccgtcct gccccctacc tgcctggggg aggagcctga gaacacgccc
     7501 gccaacagcc ggactctgac ccgggcagcc tccctgcgcg gaggggtggg ggccccgggc
     7561 tcccccagca cgccccccac acgcttcttt accgagaaga agattcccca tgagtgcctg
     7621 gtcatcggag cgcttgaaag tgcattcaag gaaatggacc tacagataga acgagagagg
     7681 agttcatata atatatctgg tggctgcacg gccctcattg tgatttgcct tttggggaag
     7741 ctgtatgttg caaatgctgg ggatagcagg gccataatca tcagaaatgg agaaattatc
     7801 cccatgtctt cagaatttac ccccgagacg gagcgccagc gacttcagta cctggcattc
     7861 atgcagcctc acttgctggg aaatgagttc acacatttgg agtttccaag gagagtacag
     7921 agaaaggagc ttggaaagaa gatgctctac agggacttta atatgacagg ctgggcatac
     7981 aaaaccattg aggatgagga cttgaagttc ccccttatat atggagaagg caagaaggcc
     8041 cgggtaatgg caactattgg agtgaccagg ggacttgggg accatgacct gaaggtgcat
     8101 gactccaaca tctacattaa accattcctg tcttcagctc cagaggtaag aatctacgat
     8161 ctttcaaaat atgatcatgg atcagatgat gtgctgatct tggccactga tggactctgg
     8221 gacgttttat caaatgaaga agtagcagaa gcaatcactc agtttcttcc taactgtgat
     8281 ccagatgatc ctcacaggta cacactggca gctcaggacc tggtgatgcg tgcccggggt
     8341 gtgctgaagg acagaggatg gcggatatct aatgaccgac tgggctcagg agacgacatt
     8401 tctgtatatg tcattccttt aatacatgga aacaagctgt cagccgccgc cgaccccggc
     8461 cggcttccac cagctgccag cgaggaagca cacaccagca atgtcaagat ggaaaaattg
     8521 tacctgcatt acttgagagc agagagcttt agaaaagctc tgatttatca aaagaagtat
     8581 cttttgctgt tgattggtgg attccaggat tctgaacaag aaacactctc catgattgcc
     8641 catttggggg tatttccttc caaagcagaa cggaaaatca catctcgtcc tttcaccagg
     8701 ttccgcacgg ccgtcagggt ggtcattgca atattaagat tacgtttttt ggttaagaaa
     8761 tggcaagaag tagatcggaa aggagctctg gcacaaggca aagcccctcg cccagggccc
     8821 cgagcacgac agccgcagtc tccacccaga accagagagt cccccccaac ccgggatgta
     8881 ccctctggcc acaccaggga ccctgccaga ggccgcagac tggcagcagc agcctcccca
     8941 cacagtgggg gaagagccac tccatcccca aattcaagat tagaaagatc cctgactgct
     9001 tctcaagatc cagaacattc cttgacagag tatattcacc atttagaagt gatccagcaa
     9061 agattgggag gggtactacc agattctact tcaaagaaat cctgccaccc gatgattaaa
     9121 cagtgtgcta gctaccctta cgacgtgcca gactacgcct aaggccgcaa ggatctgcga
     9181 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     9241 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     9301 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     9361 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     9421 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     9481 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     9541 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     9601 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     9661 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgccacc
     9721 atgagccggc tggacaagag caaagtgatc aacagcgccc tggaactgct gaacggcgtg
     9781 ggcatcgagg gcctgaccac ccggaagctg gcccagaaac tgggcgtgga acagcccacc
     9841 ctgtactggc acgtgaagaa caagcgggcc ctgctggacg ccctgcccat cgagatgctg
     9901 gaccggcacc acacccacag ctgccctctg gaaggcgaga gctggcagga cttcctgcgg
     9961 aacaacgcca agagctacag atgcgccctg ctgagccacc gggacggcgc caaagtgcac
    10021 ctgggcacca gacccaccga gaagcagtac gagacactgg aaaaccagct ggccttcctg
    10081 tgccagcagg gcttcagcct ggaaaacgcc ctgtacgccc tgagcgccgt gggccacttc
    10141 accctgggct gcgtgctgga agaacaggaa caccaggtcg c
//
