LOCUS       sequence-465065-        5718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tdTomato is expressed under the mycobacterial L5 promoter for in 
            vivo and in vitro detection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Kong Y, Cirillo JD
  TITLE     Fluorescence Imaging of Mycobacterial Infection in Live Mice Using 
            Fluorescent Protein-Expressing Strains.
  JOURNAL   Methods Mol Biol. 2018;1790:75-85. doi: 10.1007/978-1-4939-7860-1_6.
  PUBMED    29858784
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1790:75-85. doi"; pages: " 
            10.1007/978-1-4939-7860-1_6"
COMMENT     Sequence Label: pJDC60
FEATURES             Location/Qualifiers
     source          1..5718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(110..129)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             121..936
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(194..213)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1756..1775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3936..5366)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     terminator      5632..5678
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gggggggggg aaagccacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa
       61 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
      121 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
      181 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      241 tatcgcttgt atgggaagcc ccatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      301 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      361 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      421 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      481 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      541 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      601 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
      661 gaaatgcata atcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
      721 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
      781 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
      841 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
      901 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
      961 cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt
     1021 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa
     1081 agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcacc aaccgtggct
     1141 ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca gcaacacctt
     1201 cttcacgagg cagacctcac tagttccatg agcgtcagac cccgtagaaa agatcaaagg
     1261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1621 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1681 cgaggggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1981 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcacgcgtg agcccaccag
     2041 ctccgtaagt tcgggcgctg tgtggctcgt acccgcgcat tcaggcggca gggggtctaa
     2101 cgggtctaag gcggcgtgta cggccgccac agcggctctc agcggcccgg aaacgtcctc
     2161 gaaacgacgc atgtgttcct cctggttggt acaggtggtt gggggtgctc ggctgtcgct
     2221 ggtgttccac caccagggct cgacgggaga gcgggggagt gtgcagttgt ggggtggccc
     2281 ctcagcgaaa tatctgactt ggagctcgtg tcggaccata caccggtgat taatcgtggt
     2341 ctactaccaa gcgtgagcca cgtcgccgac gaatttgagc agctctggct gccgtactgg
     2401 ccgctggcaa gcgacgatct gctcgagggg atctaccgcc aaagccgcgc gtcggcccta
     2461 ggccgccggt acatcgaggc gaacccaaca gcgctggcaa acctgctggt cgtggacgta
     2521 gaccatccag acgcagcgct ccgagcgctc agcgcccggg ggtcccatcc gctgcccaac
     2581 gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag tgtgggcact caacgcccct
     2641 gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat acatggcggc gtgcgccgaa
     2701 ggccttcggc gcgccgtcga tggcgaccgc agttactcag gcctcatgac caaaaacccc
     2761 ggccacatcg cctgggaaac ggaatggctc cactcagatc tctacacact cagccacatc
     2821 gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc gtcagcagac cacgtacaaa
     2881 gcggctccga cgccgctagg gcggaattgc gcactgttcg attccgtcag gttgtgggcc
     2941 tatcgtcccg ccctcatgcg gatctacctg ccgacccgga acgtggacgg actcggccgc
     3001 gcgatctatg ccgagtgcca cgcgcgaaac gccgaatttc cgtgcaacga cgtgtgtccc
     3061 ggaccgctac cggacagcga ggtccgcgcc atcgccaaca gcatttggcg ttggatcaca
     3121 accaagtcgc gcatttgggc ggacgggatc gtggtctacg aggccacact cagtgcgcgc
     3181 cagtcggcca tctcgcggaa gggcgcagca gcgcgcacgg cggcgagcac agttgcgcgg
     3241 cgcgcaaagt ccgcgtcagc catgcatgga ggcattgcta tgagcgacgg ctacagcgac
     3301 ggctacaacc ggcagccgac tgtccgcaaa aagcggcgcg tgaccgccgc cgaaggcgct
     3361 cgaatcaccg gactatccga acgccacgtc gtccggctcg tggcgcagga acgcagcgag
     3421 tggctcgccg agcaggctgc acgccgcgaa cgcatccgcg cctatcacga cgacgagggc
     3481 cactcttggc cgcaaacggc caaacatttc gggctgcatc tggacaccgt taagcgactc
     3541 ggctatcggg cgaggaaaga gcgtgcggca gaacaggaag cggctcaaaa ggcccacaac
     3601 gaagccgaca atccaccgct gttctaacgc aattggggag cgggtgtcgc gggggttccg
     3661 tggggggttc cgttgcaacg ggtcggacag gtaaaagtcc tggtagacgc tagttttctg
     3721 gtttgggcca tgcctgtctc gttgcgtgtt tcgttgcgcc cgttttgaat accagccaga
     3781 cgagacgggg ttctacgaat cttggtcgat accaagccat ttccgctgaa tatcggggag
     3841 ctcaccgcca gaatcggtgg ttgtggtgat gtacgtggcg aactccgttg tagtgcctgt
     3901 ggtggcatcc gtggcgcggc cgcggtaccg aattcttact tgtacagctc gtccatgccg
     3961 tacaggaaca ggtggtggcg gccctcggag cgctcgtact gttccacgat ggtgtagtcc
     4021 tcgttgtggg aggtgatgtc cagcttggtg tccacgtagt agtagccggg cagttgcacg
     4081 ggcttcttgg ccatgtagat ggtcttgaac tccaccaggt agtggccgcc gtccttcagc
     4141 ttcagggcct ggtggatctc gcccttcagc acgccgtcgc gggggtacag gcgctcggtg
     4201 gaggcctccc agcccatggt cttcttctgc attacggggc cgtcgggggg gaagttggtg
     4261 ccgcgcatct tcaccttgta gatcagcgtg ccgtcctgca gggaggagtc ctgggtcacg
     4321 gtcaccagac cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag
     4381 gacagcttct tgtaatcggg gatgtcggcg gggtgcttca cgtacgcctt ggagccgtac
     4441 atgaactggg gggacaggat gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc
     4501 agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac
     4561 tcgtggccgt tcatggagcc ctccatgcgc accttgaagc gcatgaactc tttgatgacg
     4621 gccatgttgt tgtcctcgga ggaggcggtg ccggagctgc cgctgccggt gctgccggtg
     4681 ccatgcccca ggaacaggtg gtggcggccc tcggagcgct cgtactgttc cacgatggtg
     4741 tagtcctcgt tgtgggaggt gatgtccagc ttggtgtcca cgtagtagta gccgggcagt
     4801 tgcacgggct tcttggccat gtagatggtc ttgaactcca ccaggtagtg gccgccgtcc
     4861 ttcagcttca gggcctggtg gatctcgccc ttcagcacgc cgtcgcgggg gtacaggcgc
     4921 tcggtggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc gggggggaag
     4981 ttggtgccgc gcatcttcac cttgtagatc agcgtgccgt cctgcaggga ggagtcctgg
     5041 gtcacggtca ccagaccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg
     5101 gggaaggaca gcttcttgta atcggggatg tcggcggggt gcttcacgta cgccttggag
     5161 ccgtacatga actgggggga caggatgtcc caggcgaagg gcagggggcc gcccttggtc
     5221 accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc
     5281 tcgaactcgt ggccgttcat ggagccctcc atgcgcacct tgaagcgcat gaactctttg
     5341 atgacctcct cgcccttgct caccatggat ccaagcttat cgatgattcg gtcgaacgca
     5401 cgcgtctgca cgcgcatctc caggtcgacc gttcgcttca accacgccca ttcgccgtcg
     5461 tggttgatca tctccgcgac gtgaggcgga accgtcacag gacgcttcga ccggacggtc
     5521 ttggcgttgc caacgacgat ctggcgcgaa cgtgcgttct tgtgcaggta cggcagcttg
     5581 gtggctgtca actcgacgta gttaactagc gtacgatcga ctgccaggca tcaaataaaa
     5641 cgaaaggctc agtcgaaaga ctgggccttt cgttttatgc catcatggcc gcggtgatca
     5701 gctagccacc tgacgtcg
//
