LOCUS       sequence-465066-       13278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses ABE8e(V106W) - SpG Cas9 base editor in a human T cell 
            optimized lentiviral transfer plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13278)
  AUTHORS   Schmidt R, Ward CC, Dajani R, Armour-Garb Z, Ota M, Allain V, 
            Hernandez R, Layeghi M, Xing G, Goudy L, Dorovskyi D, Wang C, Chen 
            YY, Ye CJ, Shy BR, Gilbert LA, Eyquem J, Pritchard JK, Dodgson SE, 
            Marson A
  TITLE     Base-editing mutagenesis maps alleles to tune human T cell 
            functions.
  JOURNAL   Nature. 2024 Jan;625(7996):805-812. doi: 10.1038/s41586-023-06835-6.
            Epub 2023 Dec 13.
  PUBMED    38093011
REFERENCE   2  (bases 1 to 13278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06835-6"; journalName: "Nature"; date: 
            "2024-01"; volume: "625"; issue: "7996"; pages: "805-812"
COMMENT     Sequence Label: pLenti-CR102
FEATURES             Location/Qualifiers
     source          1..13278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     491..510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     744..761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(831..851)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        836..1165
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1016..1151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2299..2364
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2494..2514
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2939..3073
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2976..2995)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3030..3049
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3241..3874
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3921..4046
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4543..4776
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4961..5005
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5290..5407
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5468..5679
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5737..5757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6352..10452
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             10498..10518
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10528..10584
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10585..10983
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             11080..11313
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(11338..11356)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11339..11356)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11646..11665)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11879..11898)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11998..12020
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12058..12076)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12144..12248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12249..12317,12318..13109)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12467..12486)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
      841 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      901 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      961 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     1021 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     1081 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     1141 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
     1201 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
     1261 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
     1321 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
     1381 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
     1441 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
     1501 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     1561 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     1621 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
     1681 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     1741 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
     1801 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
     1861 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     1921 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     1981 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
     2041 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
     2101 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
     2161 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
     2221 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     2281 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     2341 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     2401 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     2461 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     2521 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     2581 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     2641 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     2701 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     2761 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     2821 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     2881 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     2941 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3001 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3061 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     3121 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
     3181 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
     3241 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
     3301 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     3361 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     3421 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     3481 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     3541 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     3601 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     3661 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     3721 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     3781 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3841 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     3901 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     3961 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     4021 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     4081 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     4141 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     4201 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     4261 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     4321 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     4381 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     4441 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     4501 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     4561 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     4621 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     4681 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     4741 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     4801 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     4861 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     4921 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     4981 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     5041 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     5101 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     5161 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     5221 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     5281 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     5341 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     5401 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     5461 cgaattcggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     5521 aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     5581 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     5641 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg ggatcccctg cagggccacc
     5701 atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtctct
     5761 gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc caagagggca
     5821 cgggatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag agtgatcggc
     5881 gagggctgga acagagccat cggcctgcac gacccaacag cccatgccga aattatggcc
     5941 ctgagacagg gcggcctggt catgcagaac tacagactga ttgacgccac cctgtacgtg
     6001 acattcgagc cttgcgtgat gtgcgccggc gccatgatcc actctaggat cggccgcgtg
     6061 gtgtttggat ggagaaattc taaaagaggc gccgcaggct ccctgatgaa cgtgctgaac
     6121 taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga tgaatgtgcc
     6181 gccctgctgt gcgatttcta tcggatgcct agacaggtgt tcaatgctca gaagaaggcc
     6241 cagagctcca tcaactccgg aggatctagc ggaggctcct ctggctctga gacacctggc
     6301 acaagcgaga gcgcaacacc tgaaagcagc gggggcagca gcggggggtc agacaagaag
     6361 tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag
     6421 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
     6481 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
     6541 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
     6601 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
     6661 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
     6721 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
     6781 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
     6841 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
     6901 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
     6961 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
     7021 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg
     7081 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
     7141 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
     7201 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
     7261 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
     7321 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
     7381 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
     7441 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
     7501 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc
     7561 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
     7621 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
     7681 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
     7741 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
     7801 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
     7861 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
     7921 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag
     7981 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg
     8041 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa
     8101 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag
     8161 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca
     8221 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac
     8281 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg
     8341 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag
     8401 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt
     8461 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt
     8521 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
     8581 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
     8641 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
     8701 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
     8761 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
     8821 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag
     8881 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
     8941 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
     9001 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
     9061 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
     9121 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
     9181 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
     9241 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
     9301 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg
     9361 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
     9421 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
     9481 tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag
     9541 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg
     9601 aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc
     9661 ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag
     9721 gactgggacc ctaagaagta cggcggcttc ctgtggccca ccgtggccta ttctgtgctg
     9781 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg
     9841 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc
     9901 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc
     9961 gagctggaaa acggccggaa gagaatgctg gcctctgcca agcagctgca gaagggaaac
    10021 gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
    10081 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
    10141 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac
    10201 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag
    10261 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc
    10321 aagtactttg acaccaccat cgaccggaag cagtacagaa gcaccaaaga ggtgctggac
    10381 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
    10441 ctgggaggtg acgagggagc tgataagcgc accgccgatg gttccgagtt cgaaagcccc
    10501 aagaagaaga ggaaagtcgg ctccggcgca acaaacttct ctctgctgaa acaagccgga
    10561 gatgtcgaag agaatcctgg accgatggcc aagcctttgt ctcaagaaga atccaccctc
    10621 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
    10681 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
    10741 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
    10801 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
    10861 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
    10921 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
    10981 tgaacgcgtc gatgagagga gtattcgtgg tacctttaag accaatgact tacaaggcag
    11041 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
    11101 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
    11161 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    11221 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    11281 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct
    11341 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
    11401 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
    11461 atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc
    11521 tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
    11581 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
    11641 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
    11701 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
    11761 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
    11821 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
    11881 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
    11941 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
    12001 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
    12061 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    12121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    12181 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    12241 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    12301 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    12361 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    12421 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    12481 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    12541 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    12601 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    12661 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    12721 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    12781 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    12841 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    12901 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    12961 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    13021 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    13081 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    13141 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    13201 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    13261 aaaagatcaa aggatctt
//
