LOCUS       sequence-465079-       10492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The cre-activatable fluorescence reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Ohta Y, Fujii M, Takahashi S, Takano A, Nanki K, Matano M, Hanyu H, 
            Saito M, Shimokawa M, Nishikori S, Hatano Y, Ishii R, Sawada K, 
            Machinaga A, Ikeda W, Imamura T, Sato T
  TITLE     Cell-matrix interface regulates dormancy in human colon cancer stem 
            cells.
  JOURNAL   Nature. 2022 Jul 7. pii: 10.1038/s41586-022-05043-y. doi: 
            10.1038/s41586-022-05043-y.
  PUBMED    35798028
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 7. pii: 10.1038/s41586-022-05043-y. doi: 
            10.1038/s41586-022-05043-y."
COMMENT     Sequence Label: tdiRFP-BFP reporter
FEATURES             Location/Qualifiers
     source          1..10492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1440..1643
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1594..1614
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1640..1664
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(1714..1747)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1762..2139
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      2152..2161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(4173..4747,4748..4750,4751..4759)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4340..4357)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4567..4586
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4760..5359
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4760..4779)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5256..5276
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5562..5573
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(6052..6762)
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             complement(6769..6789)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(6802..6834)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(6838..6855)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    6880..6913
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    7062..7183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7099..7118)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7153..7172
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7946..7980
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8471..8487)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8471..8487)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8484..8506)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8495..8511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8519..8525,8526..8543,8544..8549))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8564..8585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8702..8719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8873..9461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8953..8972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9632..10423,10424..10492))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10255..10274
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtattatgcc cagtacatga
     1381 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     1441 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     1501 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1561 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     1621 gggaggttta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     1681 cacgctgttt tgacctccat agaagattct agaataactt cgtatagcat acattatacg
     1741 aagttatcag atctcgccac catgccagag ccagcgaagt ctgctcccgc cccgaaaaag
     1801 ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc
     1861 cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca ccctgacacc
     1921 ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat tttcgagcgc
     1981 atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat cacctccagg
     2041 gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca cgccgtgtcc
     2101 gagggtacta aggccatcac caagtacacc agcgctaagg atccaccggt cgccaccatg
     2161 gccgaaggga gcgtggcaag acagcccgat ctgctgacat gcgacgacga accaatccac
     2221 attccaggag ctatccagcc tcatggactg ctgctggctc tggccgctga catgactatc
     2281 gtggcaggct ctgataacct gccagagctg accgggctgg ctatcggagc actgattggc
     2341 cggtccgcag ccgacgtgtt cgattctgag actcacaata gactgaccat cgccctggct
     2401 gaacctgggg ctgcagtggg agccccaatt accgtcggct tcacaatgcg aaaggacgct
     2461 ggctttatcg ggtcctggca ccgccatgat cagctgattt ttctggagct ggaaccacct
     2521 cagagagacg tggcagagcc acaggctttc tttcggagaa caaacagcgc catcaggcgc
     2581 ctgcaggccg ctgagactct ggaatccgct tgcgcagccg ctgcacagga agtgaggaag
     2641 atcaccgggt tcgatcgcgt catgatctac agattcgcct cagactttag cggagaagtg
     2701 atcgcagaag atcgctgtgc cgaggtcgaa agtaaactgg gcctgcatta cccagcctca
     2761 accgtgcctg cacaggcacg acggctgtat acaatcaacc ccgtgcggat cattcccgac
     2821 attaattaca gaccagtgcc cgtcacacct gacctgaacc cagtgactgg gaggcccatc
     2881 gatctgagct tcgccattct gcgcagcgtg tcccctgtcc acctggagtt tatgaggaat
     2941 atcggaatgc atggcaccat gtctatcagt attctgagag gcgaaaggct gtgggggctg
     3001 atcgtgtgcc accatcggac accttactat gtggacctgg acggcaggca ggcttgtgaa
     3061 ctggtggctc aggtgctggc ttggcagatt ggcgtgatga aggaggggca tggcaccggc
     3121 agcaccggca gcggcagcag cggccgctcc ggcaccgcct cctccgagga caacaacatg
     3181 gccgccgaag ggagcgtggc aagacagccc gatctgctga catgcgacga cgaaccaatc
     3241 cacattccag gagctatcca gcctcatgga ctgctgctgg ctctggccgc tgacatgact
     3301 atcgtggcag gctctgataa cctgccagag ctgaccgggc tggctatcgg agcactgatt
     3361 ggccggtccg cagccgacgt gttcgattct gagactcaca atagactgac catcgccctg
     3421 gctgaacctg gggctgcagt gggagcccca attaccgtcg gcttcacaat gcgaaaggac
     3481 gctggcttta tcgggtcctg gcaccgccat gatcagctga tttttctgga gctggaacca
     3541 cctcagagag acgtggcaga gccacaggct ttctttcgga gaacaaacag cgccatcagg
     3601 cgcctgcagg ccgctgagac tctggaatcc gcttgcgcag ccgctgcaca ggaagtgagg
     3661 aagatcaccg ggttcgatcg cgtcatgatc tacagattcg cctcagactt tagcggagaa
     3721 gtgatcgcag aagatcgctg tgccgaggtc gaaagtaaac tgggcctgca ttacccagcc
     3781 tcaaccgtgc ctgcacaggc acgacggctg tatacaatca accccgtgcg gatcattccc
     3841 gacattaatt acagaccagt gcccgtcaca cctgacctga acccagtgac tgggaggccc
     3901 atcgatctga gcttcgccat tctgcgcagc gtgtcccctg tccacctgga gtttatgagg
     3961 aatatcggaa tgcatggcac catgtctatc agtattctga gaggcgaaag gctgtggggg
     4021 ctgatcgtgt gccaccatcg gacaccttac tatgtggacc tggacggcag gcaggcttgt
     4081 gaactggtgg ctcaggtgct ggcttggcag attggcgtga tgaaggagga ataactcgag
     4141 agtgtacaaa gtggtgataa ttccgccccc cccccctctc cctccccccc ccctaacgtt
     4201 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     4261 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     4321 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     4381 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     4441 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     4501 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     4561 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     4621 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     4681 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     4741 tgataatatg gccacaacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     4801 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     4861 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4921 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4981 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     5041 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     5101 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     5161 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     5221 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     5281 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     5341 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     5401 acgaccccat gcatcgatga tctagagctc gctgatcagc ctcgackgta tcgatgcggg
     5461 gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc
     5521 cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg
     5581 tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccaaggaaag
     5641 gacgatgatt tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc
     5701 cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg
     5761 aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca
     5821 gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga
     5881 gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca
     5941 tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga
     6001 ataccagtca atctttcaca aattttgtaa tccagaggtt gacatatgtt aattaagctt
     6061 gtgccccagt ttgctaggga ggtcgcagta tctggccact gccacctcgt gctgctcgac
     6121 gtaggtctcg ttgttggcct ccttgattct ttccagtctg tagtccacat agtagacgcc
     6181 aggcatcttg aggttcttag cgggtttctt ggatctatat gtggtcttgg cgtttgcgat
     6241 cagatggctc ccgcccacga gcttcagggc catgtcgttt ctgccttcca ggccgccgtc
     6301 agcggggtac agcgtctcgg tgaaggcctc ccagccgagt gttttcttct gcatcacagg
     6361 gccgttggat gtgaagttca cccctctgat cttgacgttg tagatgaggc agccgtcctg
     6421 gaggctggtg tcctgggtag cggtcagcac gcccccgtct tcgtatgtgg tgactctctc
     6481 ccatgtgaag ccctcaggga aggactgctt gaagaagtcg gggatgccct gggtgtggtt
     6541 gatgaaggtc ttgctgccgt agaggaagct agtagccagg atgtcgaagg cgaaggggag
     6601 agggccgccc tcgaccacct tgattctcat ggtctgggtg ccctcgtagg gcttgccttc
     6661 gccctcggat gtgcacttga agtgatggtt gtccacggtg ccctccatgt acagcttcat
     6721 gtgcatgttc tccttaatca gctcttcgcc cttagacacc atgctcgagc cctggaagta
     6781 caggttctcg tacagatccc gacccatttg ctgtccacca gtcatgctag ccataccatg
     6841 atgatgatga tgatgagaac cccgcatggc ggtcctagga taacttcgta taatgtatgc
     6901 tatacgaagt tatcctaggg aattcgaatt taaatcggat ctcgacaatc aacctctgga
     6961 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7021 tggatacgct gctttaatgc ctttgtatca tgcgttaact aaacttgttt attgcagctt
     7081 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7141 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggaattgac
     7201 tcaaatgatg tcaattagtc tatcagaagc tcatctggtc tcccttccgg gggacaagac
     7261 atccctgttt aatatttaaa cagcagtgtt cccaaactgg gttcttatat cccttgctct
     7321 ggtcaaccag gttgcagggt ttcctgtcct cacaggaacg aagtccctaa agaaacagtg
     7381 gcagccaggt ttagccccgg aattgactgg attccttttt tagggcccat tggtatggct
     7441 ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg
     7501 aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg
     7561 gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc
     7621 gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc tgatatctat
     7681 aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa
     7741 ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc
     7801 acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg
     7861 gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga
     7921 aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg
     7981 ttaatctagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca
     8041 agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa
     8101 gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc
     8161 acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc
     8221 gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc
     8281 agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt
     8341 gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagcgagga cgggtatcct
     8401 ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcttgg
     8461 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8521 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8581 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8641 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8701 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8761 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8821 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8881 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8941 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9001 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9061 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9121 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9181 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9241 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9301 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9361 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9421 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9481 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9541 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9601 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9661 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9721 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9781 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9841 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9901 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9961 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10021 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10081 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10141 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10201 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10261 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10321 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10381 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10441 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc at
//
