LOCUS       sequence-46515-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Robles E, Woo S, Gomez TM
  TITLE     Src-dependent tyrosine phosphorylation at the tips of growth cone 
            filopodia promotes extension.
  JOURNAL   J Neurosci. 2005 Aug 17;25(33):7669-81.
  PUBMED    16107653
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2005-08-17"; volume: "25(33)"; pages: "7669-81"
COMMENT     Sequence Label: pCS2-myc-gfp-dSH2
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        58..76
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     58..75
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             122..151
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             152..862
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(179..207)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     802..822
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cattagcatg ctcgttaggg aaccgccatt ctgcctgggg acgtcggagc aagcttgatt
       61 taggtgacac tatagaatac aagctacttg ttctttttgc aggatcgatc ccatcgatat
      121 ggagcaaaag cttattagcg aggaagatct gagtaaagga gaagaacttt tcactggagt
      181 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
      241 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
      301 aaaactacct gttccatggc ccacacttgt cactactttc acttatggtg ttcaatgctt
      361 ttcaagatac ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg
      421 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga
      481 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
      541 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata
      601 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat
      661 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg
      721 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc
      781 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca
      841 tggcatggat gaactataca agaatttcga gctcaagctt
//
