LOCUS       sequence-465231-        5871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2103m is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP1787 - pAAV-AiE2103m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..5871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      1807..1816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1819..1839
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2905..3016
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3134..3274
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3134..3263
                     /label=AAV2 ITR
     rep_origin      3349..3804
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3436..3455)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3646..3667
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3821..3840)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3940..3962
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4000..4018)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4086..4190
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4191..4259,4260..5051)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4409..4428)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5222..5810
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5711..5730
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgcaaag gatatctgca gtgtcactca aagcttcagt
      181 ctgaatagga aatgtctcaa caggttgtag ttagtgataa cttagtctcc agttagtgat
      241 gctgttttag aagactttta gacctttaga agaaggagcc ttgcttaata gagtgggtca
      301 caaatggaaa attttgatgt tttgtgctct gacccccatt tttcagttca ctctccattt
      361 cctgtctttg gacacagtat gaccagccag cctccttctc ctgctgaagt gcattctgtt
      421 ttatattttc aagagaatga tctatgctag cccaacgttc agttttgaat caattgagtt
      481 aatttggtat aaaaagtaag acaaagtaaa gtagaataac aaccagcaga cagaaactca
      541 ggaaaagaat gagaagatga atatagaaca ctattataca catgtattcc ctagcaatca
      601 ctagacaatg tccaaagctc tacaatagtg tggcaagact ctacaagagc cagaaaacac
      661 aattctaaag agctgaacac aaacatcagc accatgagcg atggaggaac caagtgtttc
      721 tttgagatgc tagtagagtg aagacatacc agcaaacatc acaaaaatgt aaaataagtt
      781 gcaggggtgg gcaggacttt agggattttg attgttggtt attgcttttt ctgaaatact
      841 taaaagaatg acttaaaatt cctgttcatt gcacattgtt tctgggctga gtgtgtacat
      901 ttctacatgt gatatagggt ctcagaaaca cttccaggac actgtacacc tttcagtttg
      961 gtatttatcc atggaatttt aattgagaaa aacttttact cttctctcct tgtggctact
     1021 cccttccact gccaccgcca cttcctgtct tatccaggca atcatccaga tttgtgcaaa
     1081 caagagtttt cacaagtgaa ctcttccaac tagatcttta ggcaaattac ttcagcaata
     1141 acttttaaat caaattaata tctcaaacct aaacattcta caggccatgg ggttaacctg
     1201 aaaactcaac agtacctttt aatgttctgg ctagatctga aactcacgtt ttagtaaaat
     1261 aaaatcatta gttggtatac cctacataat agaattgttt ttttattcta cattaacttc
     1321 agagcctctt ctgaaatata atgatgtctt tcatgcttta ttgtgattaa tacaaaaact
     1381 ggattaaaca tatccatata caacttcttg gcttcatatt aggcctgaat gcagaactgg
     1441 cagcacatag cagaacaatt accaacagaa aagtcccctt tttcaaagtt cattttgtgt
     1501 ttccctttca ttacacaaaa gattagataa caaagagttt tctttgctac aaaaaattat
     1561 ctttcttcag cattataact gatggagaaa attactttgg tgaaaatgaa aatagaaagc
     1621 ataagtgtta atagatgaat tcatatctaa ttatctactt ctcgcttgca aaaaaagaaa
     1681 tagtgggata cttaagttaa aatgttctgc ccagaaatca aagacgagct cgggctgggc
     1741 ataaaagtca gggcagagcc atctattgct tacatttgct tctgggatcc agatctttcg
     1801 aagctagcca ccatggtgcc caagaagaag aggaaagtct ccaacctgct gactgtgcac
     1861 caaaacctgc ctgccctccc tgtggatgcc acctctgatg aagtcaggaa gaacctgatg
     1921 gacatgttca gggacaggca ggccttctct gaacacacct ggaagatgct cctgtctgtg
     1981 tgcagatcct gggctgcctg gtgcaagctg aacaacagga aatggttccc tgctgaacct
     2041 gaggatgtga gggactacct cctgtacctg caagccagag gcctggctgt gaagaccatc
     2101 caacagcacc tgggccagct caacatgctg cacaggagat ctggcctgcc tcgcccttct
     2161 gactccaatg ctgtgtccct ggtgatgagg agaatcagaa aggagaatgt ggatgctggg
     2221 gagagagcca agcaggccct ggcctttgaa cgcactgact ttgaccaagt cagatccctg
     2281 atggagaact ctgacagatg ccaggacatc aggaacctgg ccttcctggg cattgcctac
     2341 aacaccctgc tgcgcattgc cgaaattgcc agaatcagag tgaaggacat ctcccgcacc
     2401 gatggtggga gaatgctgat ccacattggc aggaccaaga ccctggtgtc cacagctggt
     2461 gtggagaagg ccctgtccct gggggttacc aagctggtgg agagatggat ctctgtgtct
     2521 ggtgtggctg atgaccccaa caactacctg ttctgccggg tcagaaagaa tggtgtggct
     2581 gccccttctg ccacctccca actgtccacc cgggccctgg aagggatctt tgaggccacc
     2641 caccgcctga tctatggtgc caaggatgac tctgggcaga gatacctggc ctggtctggc
     2701 cactctgcca gagtgggtgc tgccaccgac atggccaggg ctggtgtgtc catccctgaa
     2761 atcatgcagg ctggtggctg gaccaatgtg aacattgtga tgaactacat cagaaacctg
     2821 gactctgaga ctggggccat ggtgaggctg ctcgaagatg gggactgagg cgcgccgaat
     2881 tcaagcttct cgagagatct tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     2941 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3001 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3061 ggcaggacag caagggggag gattgggaag acaatagcag gcatgagatc tcacgtgcgg
     3121 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     3181 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     3241 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     3301 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     3361 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3421 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3481 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3541 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     3601 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3661 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
     3721 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3781 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     3841 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3901 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3961 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     4021 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     4081 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4141 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4201 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4261 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4321 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4381 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4441 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4501 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4561 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4621 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4681 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4741 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4801 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4861 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4921 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4981 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5041 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5101 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5161 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5221 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5281 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5341 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5401 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5461 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5521 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5581 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5641 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5701 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5761 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5821 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
