LOCUS       sequence-465232-        4904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2116m is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP1788 - pAAV-AiE2116m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      840..849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             852..872
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1938..2049
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2167..2307
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2167..2296
                     /label=AAV2 ITR
     rep_origin      2382..2837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2469..2488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2679..2700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2854..2873)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2973..2995
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3033..3051)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3119..3223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3224..3292,3293..4084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3442..3461)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4255..4843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4744..4763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttcacac ctggacgaca cctgtgaagg tcactgtcct
      181 tcactcacac ctggacacct gtgaaggtta ctgtcctcca ctcacacctg aatgacacct
      241 gtgaaagtca aggtcacaca aaaaggcact tttgtgctta tgcttctgtt ttaatctggg
      301 aacccagatg ttctatcagc atgctttatt ctgaggcccc cgcacatcct tcatgtatgc
      361 aagccttctc ttatgtaacc ttttggcttt ctgtggccag atggcttcct ctttcattca
      421 tccctatgtg aaccaactat gactccaaaa tatctggcct ttgtgtatca catatactcc
      481 agctttctgg tcttttttag gttccttgaa atatgccagg ctgtttcctc ttcctggggc
      541 ttcacagtag cagtctggta tacctgggat attttttctc ttatcctcat tatatgtgtt
      601 cctttctaaa tgtcacttct catggaatcc actttaccta atttgtgtag attttcagaa
      661 tttatgccca tgccaaagat ctttaccata actggatcta atcaccttta tgaaggtaag
      721 aaaagatccc aaatttattt agctatttca ctgagtttga gctcgggctg ggcataaaag
      781 tcagggcaga gccatctatt gcttacattt gcttctggga tccagatctt tcgaagctag
      841 ccaccatggt gcccaagaag aagaggaaag tctccaacct gctgactgtg caccaaaacc
      901 tgcctgccct ccctgtggat gccacctctg atgaagtcag gaagaacctg atggacatgt
      961 tcagggacag gcaggccttc tctgaacaca cctggaagat gctcctgtct gtgtgcagat
     1021 cctgggctgc ctggtgcaag ctgaacaaca ggaaatggtt ccctgctgaa cctgaggatg
     1081 tgagggacta cctcctgtac ctgcaagcca gaggcctggc tgtgaagacc atccaacagc
     1141 acctgggcca gctcaacatg ctgcacagga gatctggcct gcctcgccct tctgactcca
     1201 atgctgtgtc cctggtgatg aggagaatca gaaaggagaa tgtggatgct ggggagagag
     1261 ccaagcaggc cctggccttt gaacgcactg actttgacca agtcagatcc ctgatggaga
     1321 actctgacag atgccaggac atcaggaacc tggccttcct gggcattgcc tacaacaccc
     1381 tgctgcgcat tgccgaaatt gccagaatca gagtgaagga catctcccgc accgatggtg
     1441 ggagaatgct gatccacatt ggcaggacca agaccctggt gtccacagct ggtgtggaga
     1501 aggccctgtc cctgggggtt accaagctgg tggagagatg gatctctgtg tctggtgtgg
     1561 ctgatgaccc caacaactac ctgttctgcc gggtcagaaa gaatggtgtg gctgcccctt
     1621 ctgccacctc ccaactgtcc acccgggccc tggaagggat ctttgaggcc acccaccgcc
     1681 tgatctatgg tgccaaggat gactctgggc agagatacct ggcctggtct ggccactctg
     1741 ccagagtggg tgctgccacc gacatggcca gggctggtgt gtccatccct gaaatcatgc
     1801 aggctggtgg ctggaccaat gtgaacattg tgatgaacta catcagaaac ctggactctg
     1861 agactggggc catggtgagg ctgctcgaag atggggactg aggcgcgccg aattcaagct
     1921 tctcgagaga tcttcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     1981 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     2041 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     2101 cagcaagggg gaggattggg aagacaatag caggcatgag atctcacgtg cggaccgagc
     2161 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     2221 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     2281 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     2341 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     2401 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     2461 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     2521 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     2581 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     2641 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     2701 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     2761 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     2821 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2881 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     2941 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3001 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     3061 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3121 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3181 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3241 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3301 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3361 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3421 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3481 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3541 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3601 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3661 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3721 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3781 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3841 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3901 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3961 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4021 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4081 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4141 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4201 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4261 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4321 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4381 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4441 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4501 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4561 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4621 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4681 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4741 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4801 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4861 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
