LOCUS       sequence-465236-        4923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0463m is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP1794 - pAAV-AiE0463m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      859..868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             871..891
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1957..2068
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2186..2326
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2186..2315
                     /label=AAV2 ITR
     rep_origin      2401..2856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2488..2507)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2698..2719
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2873..2892)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2992..3014
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3052..3070)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3138..3242
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3243..3311,3312..4103)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3461..3480)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4274..4862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4763..4782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttttgct tgtgtgccag cagggaggag caagaggaat
      181 tttgtcactt ctctttcact ttcttcatta ttttaagttg gatctgctgt tctgttcact
      241 tttaaaatat aaccttcagt atttatgaac gtgattcatc ttttacaata tgttgtttaa
      301 catgaatgca tcttcttctt gcctttcagt gcatctttat gccattggca gccacaatca
      361 agtgtttcca ttaaagttca cagtaacagt gcagtgttgt ctgtcccaac tctgctaact
      421 tttaccaccc actatttagt tttatctacc atgtgaattt atttgtaaga tcttctttct
      481 cttatcctag ttaattatgg ggaggacttg ccaggatatt aatttacatg gcaattttgg
      541 ataagttatg catgtattca tctcctatat acaataagtt tttcttgtga acgtcaaaag
      601 aaaggataat gcatatgact gtatatgata taaactggaa tgtaatagtt gccaatagga
      661 tctgaatcaa atttcagttt ttaactatgt tctatatatc tatgtgcata tttatagcat
      721 gtgtgacata tgtataattt aaatatttct ataaacttat acagaaacaa aatatgtgag
      781 ctcgggctgg gcataaaagt cagggcagag ccatctattg cttacatttg cttctgggat
      841 ccagatcttt cgaagctagc caccatggtg cccaagaaga agaggaaagt ctccaacctg
      901 ctgactgtgc accaaaacct gcctgccctc cctgtggatg ccacctctga tgaagtcagg
      961 aagaacctga tggacatgtt cagggacagg caggccttct ctgaacacac ctggaagatg
     1021 ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc
     1081 cctgctgaac ctgaggatgt gagggactac ctcctgtacc tgcaagccag aggcctggct
     1141 gtgaagacca tccaacagca cctgggccag ctcaacatgc tgcacaggag atctggcctg
     1201 cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat
     1261 gtggatgctg gggagagagc caagcaggcc ctggcctttg aacgcactga ctttgaccaa
     1321 gtcagatccc tgatggagaa ctctgacaga tgccaggaca tcaggaacct ggccttcctg
     1381 ggcattgcct acaacaccct gctgcgcatt gccgaaattg ccagaatcag agtgaaggac
     1441 atctcccgca ccgatggtgg gagaatgctg atccacattg gcaggaccaa gaccctggtg
     1501 tccacagctg gtgtggagaa ggccctgtcc ctgggggtta ccaagctggt ggagagatgg
     1561 atctctgtgt ctggtgtggc tgatgacccc aacaactacc tgttctgccg ggtcagaaag
     1621 aatggtgtgg ctgccccttc tgccacctcc caactgtcca cccgggccct ggaagggatc
     1681 tttgaggcca cccaccgcct gatctatggt gccaaggatg actctgggca gagatacctg
     1741 gcctggtctg gccactctgc cagagtgggt gctgccaccg acatggccag ggctggtgtg
     1801 tccatccctg aaatcatgca ggctggtggc tggaccaatg tgaacattgt gatgaactac
     1861 atcagaaacc tggactctga gactggggcc atggtgaggc tgctcgaaga tggggactga
     1921 ggcgcgccga attcaagctt ctcgagagat cttcgactgt gccttctagt tgccagccat
     1981 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     2041 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     2101 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgaga
     2161 tctcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
     2221 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     2281 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
     2341 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     2401 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     2461 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     2521 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     2581 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     2641 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     2701 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
     2761 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
     2821 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     2881 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     2941 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     3001 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     3061 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     3121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3181 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3241 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3301 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3361 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3421 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3481 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3541 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3601 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3661 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3721 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3781 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3841 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3901 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3961 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4021 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4081 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4141 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4201 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4261 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4321 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4381 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4441 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4501 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4561 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4621 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4681 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4741 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4801 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4861 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4921 tgt
//
