LOCUS       sequence-465238-       10523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral dox-inducible expression of SNAP-p53DD and HRAS(G12V).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10523)
  AUTHORS   Wang NB, Adewumi HO, Lende-Dorn BA, Beitz AM, O'Shea TM, Galloway KE
  TITLE     Compact transcription factor cassettes generate functional, 
            engraftable motor neurons by direct conversion.
  JOURNAL   Cell Syst. 2025 Mar 10:101206. doi: 10.1016/j.cels.2025.101206.
  PUBMED    40086435
REFERENCE   2  (bases 1 to 10523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2025 Mar 10:101206. doi: 10.1016/j.cels.2025.101206."
COMMENT     Sequence Label: pFUW-SNAP-p53DD-IRES-HRAS(G12V)
FEATURES             Location/Qualifiers
     source          1..10523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    183..416
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             601..645
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    943..1060
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(1117..1135,1136..1158,1159..1177,1178..1200,1201..1219,
                     1220..1242,1243..1261,1262..1284,1285..1303,1304..1326,
                     1327..1345,1346..1368,1369..1387)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        1420..1458
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1455..1479
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1560..1584
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1651..1660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1657..2202
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     primer_bind     complement(2683..2700)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2910..2929
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3084..3116,3117..3119,3120..3653)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(3667..3691)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3718..4306
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3771..3791)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4189..4200)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4309..4325)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4310..4326)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4831..5011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5037..5054)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5043..5267
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5313..5741
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5400..5419)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5610..5631
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5750..5770)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5755..6084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5935..6070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5997..6016
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6132..6179
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6198..6572
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6702..6823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6739..6758)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6793..6812
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6872..6888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6872..6888)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6885..6907)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6896..6912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6920..6926,6927..6944,6945..6950))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6965..6986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7103..7120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7274..7862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7354..7373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8033..8824,8825..8893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8656..8675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8894..8998)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9073..9092)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9264..9643
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9645..9843
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9861..10041
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10088..10213
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
       61 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
      121 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
      181 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
      241 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
      301 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
      361 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
      421 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
      481 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
      541 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
      601 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
      661 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
      721 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
      781 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
      841 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
      901 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
      961 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1021 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1081 cagagatcca gtttggttag atctcgagtt taccactccc tatcagtgat agagaaaagt
     1141 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     1201 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     1261 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     1321 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     1381 atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag
     1441 gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     1501 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattatca
     1561 caagtttgta caaaaaagca ggctccacca tgggaaccaa ttcagtcgac tggatccggt
     1621 actactgaat tcaagcttgg atcccaatgt gccaccatgg acaaagactg cgaaatgaag
     1681 cgcaccaccc tggatagccc tctgggcaag ctggaactgt ctgggtgcga acagggcctg
     1741 caccgtatca tcttcctggg caaaggaaca tctgccgccg acgccgtgga agtgcctgcc
     1801 ccagccgccg tgctgggcgg accagagcca ctgatgcagg ccaccgcctg gctcaacgcc
     1861 tactttcacc agcctgaggc catcgaggag ttccctgtgc cagccctgca ccacccagtg
     1921 ttccagcagg agagctttac ccgccaggtg ctgtggaaac tgctgaaagt ggtgaagttc
     1981 ggagaggtca tcagctacag ccacctggcc gccctggccg gcaatcccgc cgccaccgcc
     2041 gccgtgaaaa ccgccctgag cggaaatccc gtgcccattc tgatcccctg ccaccgggtg
     2101 gtgcagggcg acctggacgt ggggggctac gagggcgggc tcgccgtgaa agagtggctg
     2161 ctggcccacg agggccacag actgggcaag cctgggctgg gtcctgcagg cggatccatg
     2221 actgccatgg aggagtcaca gtcggatatc agcctcaaga gagcgctgcc cacctgcaca
     2281 agcgcctctc ccccgcaaaa gaaaaaacca cttgatggag agtatttcac cctcaagatc
     2341 cgcgggcgta aacgcttcga gatgttccgg gagctgaatg aggccttaga gttaaaggat
     2401 gcccatgcta cagaggagtc tggagacagc agggctcact ccagctacct gaagaccaag
     2461 aagggccagt ctacttcccg ccataaaaaa acaatggtca agaaagtggg gcctgactca
     2521 gactgacaac accatggagc gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     2581 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     2641 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     2701 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     2761 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     2821 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     2881 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     2941 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     3001 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     3061 tggttttcct ttgaaaaaca ggaatgaccg aatacaagct ggtggtggtg ggcgccgtcg
     3121 gtgtgggcaa gagtgcgctg accatccagc tgatccagaa ccattttgtg gacgaatacg
     3181 accccactat agaggattcc taccggaagc aggtggtcat tgatggggag acgtgcctgt
     3241 tggacatcct ggataccgcc ggccaggagg agtacagcgc catgcgggac cagtacatgc
     3301 gcaccgggga gggcttcctg tgtgtgtttg ccatcaacaa caccaagtct tttgaggaca
     3361 tccaccagta cagggagcag atcaaacggg tgaaggactc ggatgacgtg cccatggtgc
     3421 tggtggggaa caagtgtgac ctggctgcac gcactgtgga atctcggcag gctcaggacc
     3481 tcgcccgaag ctacggcatc ccctacatcg aaacctcggc caagacccgg cagggagtgg
     3541 aggatgcctt ctacacgttg gtgcgtgaga tccggcagca caagctgcgg aagctgaacc
     3601 ctcctgatga gagtggcccc ggctgcatga gctgcaagtg tgtgctctcc taacgctgat
     3661 atctagaccc agctttcttg tacaaagtgg tgataattcg atatcaagct tatcgataat
     3721 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3781 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3841 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3901 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3961 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4021 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4081 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     4141 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4201 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4261 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     4321 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     4381 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     4441 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4501 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     4561 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     4621 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     4681 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     4741 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     4801 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     4861 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4921 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4981 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     5041 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5101 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5161 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5221 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5281 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5341 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5401 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5461 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5521 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5581 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5641 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5701 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     5761 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5821 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5881 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5941 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6001 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6061 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     6121 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     6181 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     6241 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     6301 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     6361 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     6421 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     6481 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     6541 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     6601 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6661 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6721 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6781 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6841 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6901 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6961 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7021 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7081 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7141 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7201 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7261 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7321 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7381 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7441 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7501 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7561 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7621 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7681 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7741 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7801 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7861 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7921 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7981 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8041 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8101 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8161 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8221 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8281 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8341 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8401 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8461 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8521 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8581 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8641 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8701 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8761 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8821 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8881 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8941 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9001 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     9061 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     9121 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     9181 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     9241 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     9301 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     9361 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     9421 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     9481 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     9541 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     9601 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     9661 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     9721 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     9781 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     9841 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9901 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9961 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    10021 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
    10081 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
    10141 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
    10201 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
    10261 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
    10321 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
    10381 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
    10441 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
    10501 agacaagata gaggaagagc aaa
//
