LOCUS       sequence-465239-        5176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2182m is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle T, Dwivedi D, Way SW, Oster
            A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya X, 
            Roth JR, Allen S, Ayala A, Bakken TE, Barcelli T, Barta S, Bendrick 
            J, Bertagnolli D, Bowlus J, Boyer G, Brouner K, Casian B, Casper T, 
            Chakka AB, Chakrabarty R, Chance RK, Chavan S, Departee M, Donadio 
            N, Dotson N, Egdorf T, Gabitto M, Garcia J, Gary A, Gasperini M, 
            Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, Halterman C, 
            Helback O, Hockemeyer D, Huang C, Huff S, Hunker A, Johansen N, 
            Juneau Z, Kalmbach B, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, 
            Lee AY, Leibly M, Lenz GH, Liang E, Lusk N, Malone J, Mollenkopf T, 
            Morin E, Newman D, Ng L, Ngo K, Omstead V, Oyama A, Pham T, Pom CA, 
            Potekhina L, Ransford S, Rette D, Rimorin C, Rocha D, Ruiz A, 
            Sanchez REA, Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shulga L, 
            Sigler AR, Siverts LA, Somasundaram S, Stewart K, Szelenyi E, Tieu 
            M, Trader C, van Velthoven CTJ, Walker M, Weed N, Wirthlin M, Wood 
            T, Wynalda B, Yao Z, Zhou T, Ariza J, Dee N, Reding M, Ronellenfitch
            K, Mufti S, Sunkin SM, Smith KA, Esposito L, Waters J, Thyagarajan 
            B, Yao S, Lein ES, Zeng H, Levi BP, Ngai J, Ting J, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types (preprint).
  JOURNAL   bioRxiv [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 
            10.1101/2024.06.10.597244.
  PUBMED    38915722
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 
            10.1101/2024.06.10.597244."
COMMENT     Sequence Label: AiP1868 - pAAV-AiE2182m-minBG-iCre-WPRE-BGHpA
FEATURES             Location/Qualifiers
     source          1..5176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      874..883
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             886..906
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2003..2023)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    2210..2321
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2439..2579
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2439..2568
                     /label=AAV2 ITR
     rep_origin      2654..3109
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2741..2760)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2951..2972
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3126..3145)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3245..3267
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3305..3323)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3391..3495
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3496..3564,3565..4356)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3714..3733)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4527..5115
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5016..5035
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgcctga gcagaacatt ttgattctca aatcaaaaca
      181 ctaggaaaga aaatgccaat tatttcataa agaaataatc actgaagtga tctttcataa
      241 aagcagagca cctagcatgt cagagggcaa gtgacattta ttgacagcac tgtgaactac
      301 atatagttct gtaagccgcc tgctgccaca ctaactgggt ttctgagtgg gaggccagag
      361 ctgtatggaa ggaaacggcg agagaaataa tggaggccaa catatgtttc tgttcaagat
      421 ccccaaagtt tactaagagt ctgtgcttat aaagggggag gcccatcccc cgccaatcca
      481 ttcttggtgc ctgttgccag cctgtaggca tatctgctgg atgctgtctc aggaatatct
      541 caagggcctc tcagcaggtt gcagtgtctt ggagaagagc agcagtagtt gacataacaa
      601 tagagccatc taaattggaa gactacaccc ctgataatct catattcagt ggcagcaaag
      661 tctgaatcag cctgcttcaa ggctgaggga ggctacatag ttccataatg ttccaatgag
      721 ttatgcatag actcattcaa cattttccat ctcccctttt gtaattaaaa tggcccgtga
      781 aagccccttt ggtagcacat actgaaagga gctcgggctg ggcataaaag tcagggcaga
      841 gccatctatt gcttacattt gcttctggga tccgccacca tggtgcccaa gaagaagagg
      901 aaagtctcca acctgctgac tgtgcaccaa aacctgcctg ccctccctgt ggatgccacc
      961 tctgatgaag tcaggaagaa cctgatggac atgttcaggg acaggcaggc cttctctgaa
     1021 cacacctgga agatgctcct gtctgtgtgc agatcctggg ctgcctggtg caagctgaac
     1081 aacaggaaat ggttccctgc tgaacctgag gatgtgaggg actacctcct gtacctgcaa
     1141 gccagaggcc tggctgtgaa gaccatccaa cagcacctgg gccagctcaa catgctgcac
     1201 aggagatctg gcctgcctcg cccttctgac tccaatgctg tgtccctggt gatgaggaga
     1261 atcagaaagg agaatgtgga tgctggggag agagccaagc aggccctggc ctttgaacgc
     1321 actgactttg accaagtcag atccctgatg gagaactctg acagatgcca ggacatcagg
     1381 aacctggcct tcctgggcat tgcctacaac accctgctgc gcattgccga aattgccaga
     1441 atcagagtga aggacatctc ccgcaccgat ggtgggagaa tgctgatcca cattggcagg
     1501 accaagaccc tggtgtccac agctggtgtg gagaaggccc tgtccctggg ggttaccaag
     1561 ctggtggaga gatggatctc tgtgtctggt gtggctgatg accccaacaa ctacctgttc
     1621 tgccgggtca gaaagaatgg tgtggctgcc ccttctgcca cctcccaact gtccacccgg
     1681 gccctggaag ggatctttga ggccacccac cgcctgatct atggtgccaa ggatgactct
     1741 gggcagagat acctggcctg gtctggccac tctgccagag tgggtgctgc cagggacatg
     1801 gccagggctg gtgtgtccat ccctgaaatc atgcaggctg gtggctggac caatgtgaac
     1861 attgtgatga actacatcag aaacctggac tctgagactg gggccatggt gaggctgctc
     1921 gaggatgggg actaagaatt cgatatcata atcaacctct ggattacaaa atttgtgaaa
     1981 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     2041 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     2101 cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2161 gggctcggct gttgggcact gacaattccg tggctcgaga gatcttcgac tgtgccttct
     2221 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2281 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2341 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2401 agcaggcatg agatctcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     2461 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     2521 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     2581 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     2641 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2701 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     2761 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     2821 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     2881 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     2941 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     3001 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     3061 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     3121 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3181 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3241 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     3301 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     3361 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3421 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3481 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3541 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3601 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3661 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3721 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3781 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3841 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3901 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3961 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4021 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4081 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4141 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4201 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4261 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4321 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4381 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4441 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4501 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4561 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4621 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4681 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4741 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4801 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4861 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4921 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4981 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5041 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5101 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5161 ccttttgctc acatgt
//
