LOCUS       sequence-465244-        5200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2182m is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP2035 - pAAV-AiE2182m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..5200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      890..899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             902..922
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2027..2047)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    2234..2345
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2463..2603
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2463..2592
                     /label=AAV2 ITR
     rep_origin      2678..3133
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2765..2784)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2975..2996
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3150..3169)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3269..3291
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3329..3347)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3415..3519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3520..3588,3589..4380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3738..3757)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4551..5139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5040..5059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgcctga gcagaacatt ttgattctca aatcaaaaca
      181 ctaggaaaga aaatgccaat tatttcataa agaaataatc actgaagtga tctttcataa
      241 aagcagagca cctagcatgt cagagggcaa gtgacattta ttgacagcac tgtgaactac
      301 atatagttct gtaagccgcc tgctgccaca ctaactgggt ttctgagtgg gaggccagag
      361 ctgtatggaa ggaaacggcg agagaaataa tggaggccaa catatgtttc tgttcaagat
      421 ccccaaagtt tactaagagt ctgtgcttat aaagggggag gcccatcccc cgccaatcca
      481 ttcttggtgc ctgttgccag cctgtaggca tatctgctgg atgctgtctc aggaatatct
      541 caagggcctc tcagcaggtt gcagtgtctt ggagaagagc agcagtagtt gacataacaa
      601 tagagccatc taaattggaa gactacaccc ctgataatct catattcagt ggcagcaaag
      661 tctgaatcag cctgcttcaa ggctgaggga ggctacatag ttccataatg ttccaatgag
      721 ttatgcatag actcattcaa cattttccat ctcccctttt gtaattaaaa tggcccgtga
      781 aagccccttt ggtagcacat actgaaagga gctcgggctg ggcataaaag tcagggcaga
      841 gccatctatt gcttacattt gcttctggga tccagatctt tcgaagctag ccaccatggt
      901 gcccaagaag aagaggaaag tctccaacct gctgactgtg caccaaaacc tgcctgccct
      961 ccctgtggat gccacctctg atgaagtcag gaagaacctg atggacatgt tcagggacag
     1021 gcaggccttc tctgaacaca cctggaagat gctcctgtct gtgtgcagat cctgggctgc
     1081 ctggtgcaag ctgaacaaca ggaaatggtt ccctgctgaa cctgaggatg tgagggacta
     1141 cctcctgtac ctgcaagcca gaggcctggc tgtgaagacc atccaacagc acctgggcca
     1201 gctcaacatg ctgcacagga gatctggcct gcctcgccct tctgactcca atgctgtgtc
     1261 cctggtgatg aggagaatca gaaaggagaa tgtggatgct ggggagagag ccaagcaggc
     1321 cctggccttt gaacgcactg actttgacca agtcagatcc ctgatggaga actctgacag
     1381 atgccaggac atcaggaacc tggccttcct gggcattgcc tacaacaccc tgctgcgcat
     1441 tgccgaaatt gccagaatca gagtgaagga catctcccgc accgatggtg ggagaatgct
     1501 gatccacatt ggcaggacca agaccctggt gtccacagct ggtgtggaga aggccctgtc
     1561 cctgggggtt accaagctgg tggagagatg gatctctgtg tctggtgtgg ctgatgaccc
     1621 caacaactac ctgttctgcc gggtcagaaa gaatggtgtg gctgcccctt ctgccacctc
     1681 ccaactgtcc acccgggccc tggaagggat ctttgaggcc acccaccgcc tgatctatgg
     1741 tgccaaggat gactctgggc agagatacct ggcctggtct ggccactctg ccagagtggg
     1801 tgctgccacc gacatggcca gggctggtgt gtccatccct gaaatcatgc aggctggtgg
     1861 ctggaccaat gtgaacattg tgatgaacta catcagaaac ctggactctg agactggggc
     1921 catggtgagg ctgctcgaag atggggactg aggcgcgccg aattcgatat cataatcaac
     1981 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     2041 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     2101 tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa ctcatcgccg
     2161 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggctc
     2221 gagagatctt cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2281 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2341 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2401 aagggggagg attgggaaga caatagcagg catgagatct cacgtgcgga ccgagcggcc
     2461 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     2521 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     2581 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     2641 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     2701 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     2761 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2821 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2881 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2941 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3001 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     3061 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3121 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     3181 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     3241 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     3301 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3361 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3421 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3481 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3541 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3601 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3661 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3721 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3781 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3841 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3901 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3961 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4021 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4081 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4141 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4201 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4261 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4321 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4381 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4441 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4501 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4561 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4621 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4681 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     4741 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4801 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4861 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4921 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4981 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5041 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5101 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5161 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
