LOCUS       sequence-465245-        4879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2538m is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP2047 - pAAV-AiE2538m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      815..824
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             827..847
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1913..2024
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2142..2282
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2142..2271
                     /label=AAV2 ITR
     rep_origin      2357..2812
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2444..2463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2654..2675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2829..2848)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2948..2970
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3008..3026)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3094..3198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3199..3267,3268..4059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3417..3436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4230..4818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4719..4738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaag agtaataatc gaccccacgt gcgtgcggag
      181 agaagctgaa tgcttttgtc tgaggccaca cagctgtgga gaggcaatgt caagggtcgg
      241 atccaggttc tgtttccctg aaagcccagg atgcagccat cagagaggcc cccatggacc
      301 acagccctta tatggctctt ctgatccaga acggaggagg aggcatgccc ctcctcgctc
      361 attccataaa tatttatcga acagagagat gtgtcagaca gactctggga ctcaggggtg
      421 agtgggctga caggtctgtg gtgtgattaa ttccctcaag gctttccagg aaggagagtt
      481 cagagtaagc agaggttcac cggcaggctt ggcagcgggc actctggggt caggggacgg
      541 gctgcaggaa cagcgtgtgc agaggccggc cgggccagca gctggtgtct tagccctgac
      601 atctttttgg caggcttccc caaatacaaa gcatttgggc tacatgatgc aaccatcttc
      661 cctgccggac caggagcttt gctagagttt cagcaaatct gcgtctcatc ctctaaatgg
      721 catcaagtac ccggagctcg ggctgggcat aaaagtcagg gcagagccat ctattgctta
      781 catttgcttc tgggatccag atctttcgaa gctagccacc atggtgccca agaagaagag
      841 gaaagtctcc aacctgctga ctgtgcacca aaacctgcct gccctccctg tggatgccac
      901 ctctgatgaa gtcaggaaga acctgatgga catgttcagg gacaggcagg ccttctctga
      961 acacacctgg aagatgctcc tgtctgtgtg cagatcctgg gctgcctggt gcaagctgaa
     1021 caacaggaaa tggttccctg ctgaacctga ggatgtgagg gactacctcc tgtacctgca
     1081 agccagaggc ctggctgtga agaccatcca acagcacctg ggccagctca acatgctgca
     1141 caggagatct ggcctgcctc gcccttctga ctccaatgct gtgtccctgg tgatgaggag
     1201 aatcagaaag gagaatgtgg atgctgggga gagagccaag caggccctgg cctttgaacg
     1261 cactgacttt gaccaagtca gatccctgat ggagaactct gacagatgcc aggacatcag
     1321 gaacctggcc ttcctgggca ttgcctacaa caccctgctg cgcattgccg aaattgccag
     1381 aatcagagtg aaggacatct cccgcaccga tggtgggaga atgctgatcc acattggcag
     1441 gaccaagacc ctggtgtcca cagctggtgt ggagaaggcc ctgtccctgg gggttaccaa
     1501 gctggtggag agatggatct ctgtgtctgg tgtggctgat gaccccaaca actacctgtt
     1561 ctgccgggtc agaaagaatg gtgtggctgc cccttctgcc acctcccaac tgtccacccg
     1621 ggccctggaa gggatctttg aggccaccca ccgcctgatc tatggtgcca aggatgactc
     1681 tgggcagaga tacctggcct ggtctggcca ctctgccaga gtgggtgctg ccaccgacat
     1741 ggccagggct ggtgtgtcca tccctgaaat catgcaggct ggtggctgga ccaatgtgaa
     1801 cattgtgatg aactacatca gaaacctgga ctctgagact ggggccatgg tgaggctgct
     1861 cgaagatggg gactgaggcg cgccgaattc aagcttctcg agagatcttc gactgtgcct
     1921 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     1981 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     2041 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     2101 aatagcaggc atgagatctc acgtgcggac cgagcggccg caggaacccc tagtgatgga
     2161 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     2221 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     2281 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     2341 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2401 acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc
     2461 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     2521 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     2581 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     2641 acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc
     2701 tattcttttg atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg
     2761 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     2821 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     2881 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2941 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     3001 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     3061 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3121 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3181 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3241 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3301 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3361 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3421 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3481 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3541 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3601 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3661 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3721 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     3781 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3841 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3901 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3961 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4021 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4081 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4141 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4201 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4261 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4321 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     4381 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4441 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4501 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4561 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4621 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4681 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4741 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4801 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4861 tggccttttg ctcacatgt
//
