LOCUS       sequence-465248-        5847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes an mCherry-tagged MCU/Mitochondrial Calcium Uniporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Bertolin G, Alves-Guerra MC, Cheron A, Burel A, Prigent C, Le Borgne
            R, Tramier M
  TITLE     Mitochondrial Aurora kinase A induces mitophagy by interacting with 
            MAP1LC3 and Prohibitin 2.
  JOURNAL   Life Sci Alliance. 2021 Apr 5;4(6):e202000806. doi: 
            10.26508/lsa.202000806. Print 2021 Jun.
  PUBMED    33820826
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-04-5"; volume: "4(6):e202000806. doi"; 
            pages: " 10.26508/lsa.202000806. Print 2021 Jun"
COMMENT     Sequence Label: MCU-mCherry
FEATURES             Location/Qualifiers
     source          1..5847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        52..355
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        356..559
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     510..530
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     604..623
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        604..622
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    656..680
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1787..1796
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1793..2503
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1938..1956)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2206..2225
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2626..2747
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2663..2682)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2717..2736
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2754..3209)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2891..2912)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3103..3122
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3236..3340
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3342..3699
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3342..3533
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3365..3385)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3550..3685
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3734..4528
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3734..4528
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3788..3807)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4398..4417
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4716..4735)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4760..4807
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5136..5724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5625..5644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      541 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
      601 aattaatacg actcactata gggagaccca agctggctag ttaagctgag catcaacaag
      661 tttgtacaaa aaagttggca tgacggccgc cgcaggtaga tcgctcctgc tgctcctctc
      721 ctctcggggc ggcggcggcg ggggcgccgg cggctgcggg gcgctgactg ccggctgctt
      781 ccctgggctg ggcgtcagcc gccaccggca gcagcagcac caccggacgg tacaccagag
      841 gatcgcttcc tggcagaatt tgggagctgt ttattgcagc actgttgtgc cctctgatga
      901 tgttacagtg gtttatcaaa atgggttacc tgtgatatct gtgaggctac catcccggcg
      961 tgaacgctgt cagttcacac tcaagcctat ctctgactct gttggtgtat ttttacgaca
     1021 actgcaagaa gaggatcggg gaattgacag agttgctatc tattcaccag atggtgttcg
     1081 cgttgctgct tcaacaggaa tagacctcct cctccttgat gactttaagc tggtcattaa
     1141 tgacttaaca taccacgtac gaccaccaaa aagagacctc ttaagtcatg aaaatgcagc
     1201 aacgctgaat gatgtaaaga cattggtcca gcaactatac accacactgt gcattgagca
     1261 gcaccagtta aacaaggaaa gggagcttat tgaaagacta gaggatctca aagagcagct
     1321 ggctcccctg gaaaaggtac gaattgagat tagcagaaaa gctgagaaga ggaccacttt
     1381 ggtgctatgg ggtggccttg cctacatggc cacacagttt ggcattttgg cccggcttac
     1441 ctggtgggaa tattcctggg acatcatgga gccagtaaca tacttcatca cttatggaag
     1501 tgccatggca atgtatgcat attttgtaat gacacgccag gaatatgttt atccagaagc
     1561 cagagacaga caatacttac tatttttcca taaaggagcc aaaaagtcac gttttgacct
     1621 agagaaatac aatcaactca aggatgcaat tgctcaggca gaaatggacc ttaagagact
     1681 gagagaccca ttacaagtac atctgcctct ccgacaaatt ggtgaaaaag attgcaagct
     1741 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag
     1801 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     1861 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     1921 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     1981 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     2041 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     2101 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     2161 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     2221 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     2281 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     2341 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     2401 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     2461 gggccgccac tccaccggcg gcatggacga gctgtacaag taaagcggcc gcgactctag
     2521 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     2581 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     2641 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2701 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
     2761 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     2821 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     2881 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     2941 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3001 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     3061 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3121 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3181 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     3241 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3301 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     3361 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3421 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3481 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3541 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3601 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3661 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     3721 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3781 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     3841 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3901 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     3961 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4021 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4081 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4141 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4201 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4261 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4321 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4381 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4441 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4501 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     4561 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4621 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     4681 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     4741 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     4801 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     4861 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     4921 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     4981 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5041 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5101 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5161 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5221 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5281 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5341 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5401 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5461 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5521 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5581 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5641 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5701 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5761 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5821 cgtattaccg ccatgcatta gttatta
//
