LOCUS       sequence-465251-        6995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes an mCherry-tagged MIC60/IMMT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Bertolin G, Alves-Guerra MC, Cheron A, Burel A, Prigent C, Le Borgne
            R, Tramier M
  TITLE     Mitochondrial Aurora kinase A induces mitophagy by interacting with 
            MAP1LC3 and Prohibitin 2.
  JOURNAL   Life Sci Alliance. 2021 Apr 5;4(6):e202000806. doi: 
            10.26508/lsa.202000806. Print 2021 Jun.
  PUBMED    33820826
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-04-5"; volume: "4(6):e202000806. doi"; 
            pages: " 10.26508/lsa.202000806. Print 2021 Jun"
COMMENT     Sequence Label: MIC60-mCherry
FEATURES             Location/Qualifiers
     source          1..6995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     303..322
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(621..640)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    665..712
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1041..1629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1530..1549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1804..2107
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2108..2311
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2262..2282
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3133..3142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4687..4696
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4693..5403
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4838..4856)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5106..5125
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5526..5647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5563..5582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5617..5636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5654..6109)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5791..5812)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6003..6022
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6136..6240
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6242..6599
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6242..6433
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6265..6285)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6450..6585
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(6634..6995,1..433)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(6634..6995,1..433)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6688..6707)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
       61 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      121 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
      181 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
      241 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
      301 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
      361 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
      421 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
      481 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
      541 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
      601 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
      661 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
      721 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
      781 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
      841 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
      901 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
      961 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1021 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1081 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1141 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1201 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1261 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1321 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1381 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1441 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1501 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1561 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1621 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1681 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     1741 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1801 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1861 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1921 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1981 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2041 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2101 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2161 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2221 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2281 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
     2341 ctaccggact cagatctcga gatgctgcgg gcctgtcagt tatcgggtgt gaccgccgcc
     2401 gcccagagtt gtctctgtgg gaagtttgtc ctccgtccat tgcgaccatg ccgcagatac
     2461 tctacttcag gcagctctgg gttgactact ggcaaaattg ctggagctgg ccttttgttt
     2521 gttggtggag gtattggtgg cactatccta tatgccaaat gggattccca tttccgggaa
     2581 agtgtagaga aaaccatacc ttactcagac aaactcttcg agatggttct tggtcctgca
     2641 gcttataatg ttccattgcc aaagaaatcg attcagtcgg gtccactaaa aatctctagt
     2701 gtatcagaag taatgaaaga atctaaacag cctgcctcac aactccaaaa acaaaaggga
     2761 gatactccag cttcagcaac agcacctaca gaagcggctc aaattatttc tgcagcaggt
     2821 gataccctgt cggtcccagc ccctgcagtt cagcctgagg aatctttaaa aactgatcac
     2881 cctgaaattg gtgaaggaaa acccacacct gcactttcag aagaagcatc ctcatcttct
     2941 ataagggagc gaccacctga agaagttgca gctcgccttg cacaacagga aaaacaagaa
     3001 caagttaaaa ttgagtctct agccaagagc ttagaagatg ctctgaggca aactgcaagt
     3061 gtcactctgc aggctattgc agctcagaat gctgcggtcc aggctgtcaa tgcacactcc
     3121 aacatattga aagccgccat ggacaattct gagattgcag gcgagaagaa atctgctcag
     3181 tggcgcacag tggagggtgc attgaaggaa cgcagaaagg cagtagatga agctgccgat
     3241 gcccttctca aagccaaaga agagttagag aagatgaaaa gtgtgattga aaatgcaaag
     3301 aaaaaagagg ttgctggggc caagcctcat ataactgctg cagagggtaa acttcacaac
     3361 atgatagttg atctggataa tgtggtcaaa aaggtccaag cagctcagtc tgaggctaag
     3421 gttgtatctc agtatcatga gctggtggtc caagctcggg atgactttaa acgagagctg
     3481 gacagtatta ctccagaagt ccttcctggg tggaaaggaa tgagtgtttc agacttagct
     3541 gacaagctct ctactgatga tctgaactcc ctcattgctc atgcacatcg tcgtattgat
     3601 cagctgaaca gagagctggc agaacagaag gccaccgaaa agcagcacat cacgttagcc
     3661 ttggagaaac aaaagctgga agaaaagcgg gcatttgact ctgcagtagc aaaagcatta
     3721 gaacatcaca gaagtgaaat acaggctgaa caggacagaa agatagaaga agtcagagat
     3781 gccatggaaa atgaaatgag aacccagctt cgccgacagg cagctgccca cactgatcac
     3841 ttgcgagatg tccttagggt acaagaacag gaattgaagt ctgaatttga gcagaacctg
     3901 tctgagaaac tctctgaaca agaattacaa tttcgtcgtc tcagtcaaga gcaagttgac
     3961 aactttactc tggatataaa tactgcctat gccagactca gaggaatcga acaggctgtt
     4021 cagagccatg cagttgctga agaggaagcc agaaaagccc accaactctg gctttcagtg
     4081 gaggcattaa agtacagcat gaagacctca tctgcagaaa cacctactat cccgctgggt
     4141 agtgcggttg aggccatcaa agccaactgt tctgataatg aattcaccca agctttaacc
     4201 gcagctatcc ctccagagtc cctgacccgt ggggtgtaca gtgaagagac ccttagagcc
     4261 cgtttctatg ctgttcaaaa actggcccga agggtagcaa tgattgatga aaccagaaat
     4321 agcttgtacc agtacttcct ctcctaccta cagtccctgc tcctattccc acctcagcaa
     4381 ctgaagccgc ccccagagct ctgccctgag gatataaaca catttaaatt actgtcatat
     4441 gcttcctatt gcattgagca tggtgatctg gagctagcag caaagtttgt caatcagctg
     4501 aagggggaat ccagacgagt ggcacaggac tggctgaagg aagcccgaat gaccctagaa
     4561 acgaaacaga tagtggaaat cctgacagca tatgccagcg ccgtaggaat aggaaccact
     4621 caggtgcagc cagagaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca
     4681 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     4741 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     4801 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     4861 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     4921 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     4981 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     5041 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     5101 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     5161 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     5221 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     5281 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     5341 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     5401 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
     5461 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     5521 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5641 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     5701 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     5761 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     5821 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     5881 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     5941 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     6001 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6061 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     6121 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6181 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6241 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     6301 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     6361 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6421 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6481 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     6541 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     6601 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     6661 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6721 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6781 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     6841 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6901 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6961 gccgagaaag tatccatcat ggctgatgca atgcg
//
