LOCUS       sequence-465252-        5537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes an mCherry-tagged Prohibitin1/PHB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Bertolin G, Alves-Guerra MC, Cheron A, Burel A, Prigent C, Le Borgne
            R, Tramier M
  TITLE     Mitochondrial Aurora kinase A induces mitophagy by interacting with 
            MAP1LC3 and Prohibitin 2.
  JOURNAL   Life Sci Alliance. 2021 Apr 5;4(6):e202000806. doi: 
            10.26508/lsa.202000806. Print 2021 Jun.
  PUBMED    33820826
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-04-5"; volume: "4(6):e202000806. doi"; 
            pages: " 10.26508/lsa.202000806. Print 2021 Jun"
COMMENT     Sequence Label: PHB1-mCherry
FEATURES             Location/Qualifiers
     source          1..5537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     303..322
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(621..640)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    665..712
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1041..1629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1530..1549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1804..2107
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2108..2311
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2262..2282
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3229..3238
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3235..3945
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3380..3398)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3648..3667
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4068..4189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4105..4124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4159..4178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4196..4651)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4333..4354)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4545..4564
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4678..4782
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4784..5141
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4784..4975
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4807..4827)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4992..5127
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5176..5537,1..433)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(5176..5537,1..433)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5230..5249)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
       61 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      121 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
      181 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
      241 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
      301 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
      361 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
      421 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
      481 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
      541 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
      601 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
      661 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
      721 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
      781 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
      841 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
      901 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
      961 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1021 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1081 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1141 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1201 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1261 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1321 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1381 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1441 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1501 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1561 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1621 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1681 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     1741 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1801 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1861 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1921 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1981 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2041 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2101 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2161 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2221 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2281 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
     2341 ctaccggact cagatctcga gatggctgcc aaagtgtttg agtccattgg caagtttggc
     2401 ctggccttag ctgttgcagg aggcgtggtg aactctgcct tatataatgt ggatgctggg
     2461 cacagagctg tcatctttga ccgattccgt ggagtgcagg acattgtggt aggggaaggg
     2521 actcattttc tcatcccgtg ggtacagaaa ccaattatct ttgactgccg ttctcgacca
     2581 cgtaatgtgc cagtcatcac tggtagcaaa gatttacaga atgtcaacat cacactgcgc
     2641 atcctcttcc ggcctgtcgc cagccagctt cctcgcatct tcaccagcat cggagaggac
     2701 tatgatgagc gtgtgctgcc gtccatcaca actgagatcc tcaagtcagt ggtggctcgc
     2761 tttgatgctg gagaactaat cacccagaga gagctggtct ccaggcaggt gagcgacgac
     2821 cttacagagc gagccgccac ctttgggctc atcctggatg acgtgtcctt gacacatctg
     2881 accttcggga aggagttcac agaagcggtg gaagccaaac aggtggctca gcaggaagca
     2941 gagagggcca gatttgtggt ggaaaaggct gagcaacaga aaaaggcggc catcatctct
     3001 gctgagggcg actccaaggc agctgagctg attgccaact cactggccac tgcaggggat
     3061 ggcctgatcg agctgcgcaa gctggaagct gcagaggaca tcgcgtacca gctctcacgc
     3121 tctcggaaca tcacctacct gccagcgggg cagtccgtgc tcctccagct gccccagaag
     3181 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
     3241 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     3301 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     3361 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     3421 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     3481 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     3541 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     3601 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     3661 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     3721 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     3781 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     3841 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     3901 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     3961 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     4021 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     4081 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4141 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
     4201 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     4261 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     4321 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     4381 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     4441 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     4501 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     4561 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     4621 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     4681 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4741 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     4801 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4861 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4921 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4981 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5041 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5101 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     5161 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5221 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5281 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5341 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5401 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5461 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5521 atggctgatg caatgcg
//
