LOCUS       sequence-465253-        6295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes an mCherry-tagged ATP5F1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Sharma RK, Chafik A, Bertolin G
  TITLE     Aurora kinase A/AURKA functionally interacts with the mitochondrial 
            ATP synthase to regulate energy metabolism and cell death.
  JOURNAL   Cell Death Discov. 2023 Jun 29;9(1):203. doi: 
            10.1038/s41420-023-01501-2.
  PUBMED    37386025
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Discov."; date: "2023-06-29"; volume: "9(1):203. doi"; pages: " 
            10.1038/s41420-023-01501-2"
COMMENT     Sequence Label: ATP5F1B-pmCherry
FEATURES             Location/Qualifiers
     source          1..6295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2244..2253
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2250..2960
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2395..2413)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2663..2682
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3083..3204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3120..3139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3174..3193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3211..3666)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3348..3369)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3560..3579
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3693..3797
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3799..4156
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3799..3990
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3822..3842)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4007..4142
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4191..4985
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4191..4985
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4245..4264)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4855..4874
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5173..5192)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5217..5264
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5593..6181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6082..6101
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctatgttg gggtttgtgg gtcgggtggc cgctgctccg gcctccgggg
      661 ccttgcggag actcacccct tcagcgtcgc tgcccccagc tcagctctta ctgcgggccg
      721 ctccgacggc ggtccatcct gtcagggact atgcggcgca aacatctcct tcgccaaaag
      781 caggcgccgc caccgggcgc atcgtggcgg tcattggcgc agtggtggac gtccagtttg
      841 atgagggact accaccaatt ctaaatgccc tggaagtgca aggcagggag accagactgg
      901 ttttggaggt ggcccagcat ttgggtgaga gcacagtaag gactattgct atggatggta
      961 cagaaggctt ggttagaggc cagaaagtac tggattctgg tgcaccaatc aaaattcctg
     1021 ttggtcctga gactttgggc agaatcatga atgtcattgg agaacctatt gatgaaagag
     1081 gtcccatcaa aaccaaacaa tttgctccca ttcatgctga ggctccagag ttcatggaaa
     1141 tgagtgttga gcaggaaatt ctggtgactg gtatcaaggt tgtcgatctg ctagctccct
     1201 atgccaaggg tggcaaaatt gggctttttg gtggtgctgg agttggcaag actgtactga
     1261 tcatggagtt aatcaacaat gtcgccaaag cccatggtgg ttactctgtg tttgctggtg
     1321 ttggtgagag gacccgtgaa ggcaatgatt tataccatga aatgattgaa tctggtgtta
     1381 tcaacttaaa agatgccacc tctaaggtag cactggtata tggtcaaatg aatgaaccac
     1441 ctggtgctcg tgcccgggta gctctgactg ggctgactgt ggctgaatac ttcagagacc
     1501 aagaaggtca agatgtactg ctatttattg ataacatctt tcgcttcacc caggctggtt
     1561 cagaggtgtc tgcattattg ggccgaatcc cttctgctgt gggctatcag cctaccctgg
     1621 ccactgacat gggtactatg caggaaagaa ttaccactac caagaaggga tctatcacct
     1681 ctgtacaggc tatctatgtg cctgctgatg acttgactga ccctgcccct gctactacgt
     1741 ttgcccattt ggatgctacc actgtactgt cgcgtgccat tgctgagctg ggcatctatc
     1801 cagctgtgga tcctctagac tccacctctc gtatcatgga tcccaacatt gttggcagtg
     1861 agcattacga tgttgcccgt ggggtgcaaa agatcctgca ggactacaaa tccctccagg
     1921 atatcattgc catcctgggt atggatgaac tttctgagga agacaagttg accgtgtccc
     1981 gtgcacggaa aatacagcgt ttcttgtctc agccattcca ggttgctgag gtcttcacag
     2041 gtcatatggg gaagctggta cccctgaagg agaccatcaa aggattccag cagattttgg
     2101 caggtgaata tgaccatctc ccagaacagg ccttctatat ggtgggaccc attgaagaag
     2161 ctgtggcaaa agctgataag ctggctgaag agcattcatc ggaattccag tcgacggtac
     2221 cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
     2281 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     2341 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     2401 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     2461 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     2521 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     2581 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     2641 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     2701 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     2761 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     2821 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     2881 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     2941 tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt
     3001 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3061 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3121 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3181 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat
     3241 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3301 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3361 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3421 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3481 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3541 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3601 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3661 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3721 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3781 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     3841 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3901 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3961 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4021 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4081 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4141 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4201 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4261 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4321 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     4381 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4441 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4501 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4561 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4621 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4681 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     4741 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4801 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4861 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4921 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     4981 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5041 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5101 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
     5161 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     5221 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
     5281 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     5341 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     5401 caacgtcggg gcgacaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
     5461 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5521 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5581 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5641 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5701 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5761 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5821 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5881 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5941 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6001 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6061 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6121 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6181 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6241 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
