LOCUS       sequence-465272-        5745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  macrophage-specific promoter driving BFP in a Tol2 backbone for 
            expression in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Thrikawala SU, Anderson MH, Rosowski EE
  TITLE     Glucocorticoids Suppress NF-kappaB-Mediated Neutrophil Control of 
            Aspergillus fumigatus Hyphal Growth.
  JOURNAL   J Immunol. 2024 Oct 1;213(7):971-987. doi: 10.4049/jimmunol.2400021.
  PUBMED    39178124
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4049/jimmunol"; 
            journalName: "J Immunol"; date: "2024-10-1- 1"; volume: "213"; 
            issue: "7"; pages: "971-987"
COMMENT     Sequence Label: Tol2-mfap4-BFP
FEATURES             Location/Qualifiers
     source          1..5745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     53..74
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        237..341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(342..410,411..1202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(560..579)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1373..1961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1862..1881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2115..2132
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2249..2270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2285..2290,2291..2308,2309..2315)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2323..2339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2328..2350
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2347..2363
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2347..2363
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2382..2402
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2384..2402
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(2457..2468,2469..2656)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    2747..2881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2784..2803)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2838..2857
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(5253..5390,5391..5402)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
       61 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
      121 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
      181 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
      241 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      301 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      361 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
      421 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
      481 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
      541 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
      601 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
      661 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
      721 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
      781 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
      841 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
      901 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
      961 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1021 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     1081 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1141 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     1201 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1261 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1321 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1381 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1441 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1501 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1561 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1621 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1681 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1741 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1801 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1861 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1921 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1981 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     2041 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     2101 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     2161 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     2221 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     2281 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     2341 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     2401 gggaacaaaa gatctagtgg atctgctggg cttgctgaag gtagggggtc aagaaccaga
     2461 ggtgtaaagt acttgagtaa ttttacttga ttactgtact taagtattat ttttggggat
     2521 ttttacttta cttgagtaca attaaaaatc aatactttta cttttactta attacatttt
     2581 tttagaaaaa aaagtacttt ttactcctta caattttatt tacagtcaaa aagtacttat
     2641 tttttggaga tcacttcatt ctattttccc ttgcggccgc gaattaaaaa acctcccaca
     2701 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     2761 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     2821 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     2881 ctacgtaata cgactcacta tagttctaga tcatcgactc acttgtgccc cagtttgcta
     2941 gggaggtcgc agtatctggc cactgccacc tcgtgctgct cgacgtaggt ctcgttgttg
     3001 gcctccttga ttctttccag tctgtagtcc acatagtaga cgccaggcat cttgaggttc
     3061 ttagcgggtt tcttggatct atatgtggtc ttgatgtttg cgatcagatg gctcccgccc
     3121 acgagcttca gggccatgtc gtttctgcct tccaggccgc cgtcagcggg gtacagcgtc
     3181 tcggtgaagg cctcccagcc gagtgttttc ttctgcatca cagggccgtt ggatgtgaag
     3241 ttcacccctc tgatcttgac gttgtagatg aggcagccgt cctggaggct ggtgtcctgg
     3301 gtagcggtca gcacgccccc gtcttcgtat gtggtgactc tctcccatgt gaagccctca
     3361 gggaaggact gcttgaagaa gtcggggatg ccctgggtgt ggttgatgaa ggtcttgctg
     3421 ccgtagagga agctagtagc caggatgtcg aaggcgaagg ggagagggcc gccctcgacc
     3481 accttgattc tcatggtctg ggtgccctcg tagggcttgc cttcgccctc ggatgtgcac
     3541 tcgaagtgat ggttgtccac ggtgccctcc atgtacagct tcatgtgcat gttctcctta
     3601 atcagctcgc tcatggtggc gaggtaccgt cgaccacgat ctaaagtcat gaagaaagaa
     3661 atcaccaata cgagacaaaa tggcgtgtgt aaatgtgatc gtcacaagga ataaaacagc
     3721 cacagaactt actgcaatct tctcactctc tcctcaacag ctcagtttat ctacaagaga
     3781 tgtaaaagag cttcactcac accttcttat tgttgcaggt tatttcttcg tataatctcg
     3841 ctctacagaa taagtcactc accgtgtgtg ttcagctgga aataactcaa acgctccttt
     3901 aaatcgctga tgctgagtct cgtgctctgt gacaaaacct gcttgaggca atcctcaaaa
     3961 acccaacaat ctgttttcac aaccgaagag tgaaaggaca gcagttctcg gttctgtttt
     4021 ctgccaaatc tgcaaacgca gacaaatgtt atgataaccc caccaaataa cccttttctg
     4081 cagaagataa caaacacacg attcaacctg cacaacacta atgacttttt tcaaaacaac
     4141 tataaaacat tggttggtta aaagcttggt tgaggagtaa aaggacacaa tgcagagata
     4201 agtgcaatgt taacctggag tctgcattta caagactcta aattctctaa cacaagaacc
     4261 agtcgagtgt tctctttggg ttaacattga ctgattcagc atacacaaga atacctgaag
     4321 tgacagagct aatcaaatgg cgcaagttaa gagcgaacac taagaatctg gctgtaaatc
     4381 cactcgattt cttaatattt ctaatttata tggatgatct gattatcaga catatctgca
     4441 tgtgtgaatc cctcagtgaa aaattgtttg cactctttaa catcagaccc ttcttaagag
     4501 atcatactgc acaacattta gtccaaaaag ctggactatt gcaatgctct tctagctgga
     4561 ctcctgcctt gcacccataa acctctacag acaatccaga aagctgcagc atatgtaatg
     4621 tgatttattt gaacagatga tgatcagaaa aaagtgtgcg tgatgggacg catcaatgga
     4681 ctttgcagca tttaattcca gcacactgta aaacatctga aatgttgttt tgcttattgt
     4741 aaaatgcctt aaagtctgtc atcacagtgg ttcagtatta ttattattat tattacctcc
     4801 agaactgtct tgagcatccc aaagagcatc tctcaccttc aaaagccaca gcgttgcatt
     4861 tattgtgtgt tgtctttgtc tcattagatg gaaactctgc tttatccaca aatgctttat
     4921 gcccttttac agttttgtac attaatccag ttcccattat ttgaatggaa aatgagcttt
     4981 tgtttcaaag aatagcagga cacaatctca taaatctgca tacaggagtt tattaaagct
     5041 ttaaagtgaa aacaaagaga aaaatgtcat tacaccaaca gccatacatt tcagactcga
     5101 tttttcagat acacatttaa aagagaagag gtttcctttt aaaagccctg tttctaaggc
     5161 acttgtttga tcttgatgct gatggttttc atactgaccg cgttattccc tttccagctg
     5221 taccaagaaa cgcgctagcc ttttacttcc ttaatactca agtacaattt taatggagta
     5281 cttttttact tttactcaag taagattcta gccagatact tttactttta attgagtaaa
     5341 attttcccta agtacttgta ctttcacttg agtaaaattt ttgagtactt tttacacctc
     5401 tgtcaagaac tcctggacaa acctctgacc tgtgtggaac agagtggata tgggtgtctg
     5461 aacagatatt cacgtctttt gcagatcaga gggcatttct ggtgttgtgc aatgacctgg
     5521 gtccaacagg taggcgtact cgtatccaag agcattgttg gacaggaggt agtctccatt
     5581 cctataaaga ggaaggaatg gcaccatgta gtagccgtca ttgtggccgg ctttccccgt
     5641 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5701 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcg
//
