LOCUS       sequence-465274-        5030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  macrophage-specific promoter in a Tol2 backbone for expression in 
            zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Thrikawala SU, Anderson MH, Rosowski EE
  TITLE     Glucocorticoids Suppress NF-kappaB-Mediated Neutrophil Control of 
            Aspergillus fumigatus Hyphal Growth.
  JOURNAL   J Immunol. 2024 Oct 1;213(7):971-987. doi: 10.4049/jimmunol.2400021.
  PUBMED    39178124
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4049/jimmunol"; 
            journalName: "J Immunol"; date: "2024-10-1- 1"; volume: "213"; 
            issue: "7"; pages: "971-987"
COMMENT     Sequence Label: Tol2-mfap4
FEATURES             Location/Qualifiers
     source          1..5030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(162..183)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(579..590,591..728)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    2385..2519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2409..2428)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2463..2482
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2610..2797,2798..2809)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2864..2884)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2864..2882)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2903..2919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2903..2919)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2916..2938)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2927..2943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2951..2957,2958..2975,2976..2981))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2996..3017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3134..3151)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3305..3893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3385..3404)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4064..4855,4856..4924))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4687..4706
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4925..5029)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
       61 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
      121 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
      181 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
      241 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
      301 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggccacaa
      361 tgacggctac tacatggtgc cattccttcc tctttatagg aatggagact acctcctgtc
      421 caacaatgct cttggatacg agtacgccta cctgttggac ccaggtcatt gcacaacacc
      481 agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca tatccactct
      541 gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa
      601 aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat taaaagtaaa
      661 agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta aaattgtact
      721 tgagtattaa ggaagtaaaa ggctagcgcg tttcttggta cagctggaaa gggaataacg
      781 cggtcagtat gaaaaccatc agcatcaaga tcaaacaagt gccttagaaa cagggctttt
      841 aaaaggaaac ctcttctctt ttaaatgtgt atctgaaaaa tcgagtctga aatgtatggc
      901 tgttggtgta atgacatttt tctctttgtt ttcactttaa agctttaata aactcctgta
      961 tgcagattta tgagattgtg tcctgctatt ctttgaaaca aaagctcatt ttccattcaa
     1021 ataatgggaa ctggattaat gtacaaaact gtaaaagggc ataaagcatt tgtggataaa
     1081 gcagagtttc catctaatga gacaaagaca acacacaata aatgcaacgc tgtggctttt
     1141 gaaggtgaga gatgctcttt gggatgctca agacagttct ggaggtaata ataataataa
     1201 taatactgaa ccactgtgat gacagacttt aaggcatttt acaataagca aaacaacatt
     1261 tcagatgttt tacagtgtgc tggaattaaa tgctgcaaag tccattgatg cgtcccatca
     1321 cgcacacttt tttctgatca tcatctgttc aaataaatca cattacatat gctgcagctt
     1381 tctggattgt ctgtagaggt ttatgggtgc aaggcaggag tccagctaga agagcattgc
     1441 aatagtccag ctttttggac taaatgttgt gcagtatgat ctcttaagaa gggtctgatg
     1501 ttaaagagtg caaacaattt ttcactgagg gattcacaca tgcagatatg tctgataatc
     1561 agatcatcca tataaattag aaatattaag aaatcgagtg gatttacagc cagattctta
     1621 gtgttcgctc ttaacttgcg ccatttgatt agctctgtca cttcaggtat tcttgtgtat
     1681 gctgaatcag tcaatgttaa cccaaagaga acactcgact ggttcttgtg ttagagaatt
     1741 tagagtcttg taaatgcaga ctccaggtta acattgcact tatctctgca ttgtgtcctt
     1801 ttactcctca accaagcttt taaccaacca atgttttata gttgttttga aaaaagtcat
     1861 tagtgttgtg caggttgaat cgtgtgtttg ttatcttctg cagaaaaggg ttatttggtg
     1921 gggttatcat aacatttgtc tgcgtttgca gatttggcag aaaacagaac cgagaactgc
     1981 tgtcctttca ctcttcggtt gtgaaaacag attgttgggt ttttgaggat tgcctcaagc
     2041 aggttttgtc acagagcacg agactcagca tcagcgattt aaaggagcgt ttgagttatt
     2101 tccagctgaa cacacacggt gagtgactta ttctgtagag cgagattata cgaagaaata
     2161 acctgcaaca ataagaaggt gtgagtgaag ctcttttaca tctcttgtag ataaactgag
     2221 ctgttgagga gagagtgaga agattgcagt aagttctgtg gctgttttat tccttgtgac
     2281 gatcacattt acacacgcca ttttgtctcg tattggtgat ttctttcttc atgactttag
     2341 atcgtggtcg actgatctag aactatagtg agtcgtatta cgtagatcca gacatgataa
     2401 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     2461 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     2521 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     2581 aattcgcggc cgcaagggaa aatagaatga agtgatctcc aaaaaataag tactttttga
     2641 ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt
     2701 aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat
     2761 acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg ttcttgaccc
     2821 cctaccttca gcaagcccag cagatccact agatcttttg ttccctttag tgagggttaa
     2881 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2941 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3001 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3061 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3121 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3181 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3241 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3301 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3361 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3421 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3481 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3541 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3601 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3661 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3721 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3781 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3841 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3901 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3961 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4021 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4081 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4141 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4201 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4261 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4321 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4381 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4441 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4501 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4561 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4621 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4681 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4741 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4801 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4861 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4921 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4981 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
//
