LOCUS       sequence-465387-        1173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells
            with localization in the nucleoli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1173)
  TITLE     Rivera-Fuentes plasmids March 2025
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NoLS-Grx1-roGFP2
FEATURES             Location/Qualifiers
     source          1..1173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..24
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(457..459,460..1173)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(499..520)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(760..779)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1107..1128
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgccaaaga agaagcgcaa ggtgccggtg caccgccgcc gccgccgccg ccgccagtcg
       61 accggcagca tggctcaaga gtttgtgaac tgcaaaatcc agcctgggaa ggtggttgtg
      121 ttcatcaagc ccacctgccc gtactgcagg agggcccaag agatcctcag tcaattgccc
      181 atcaaacaag ggcttctgga atttgtcgat atcacagcca ccaaccacac taacgagatt
      241 caagattatt tgcaacagct cacgggagca agaacggtgc ctcgagtctt tattggtaaa
      301 gattgtatag gcggatgcag tgatctagtc tctttgcaac agagtgggga actgctgacg
      361 cggctaaagc agattggagc tctgcagact gaggaggagg ccggcgagaa gatcaaggag
      421 ctggagaaga aggccaggaa gctgcccgag gaattcgtga gcaagggcga ggagctgttc
      481 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      541 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctcc
      601 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
      661 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      721 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      781 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      841 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caactgccac
      901 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
      961 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1021 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacctgctc cgccctgagc
     1081 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1141 atcactctcg gcatggacga gctgtacaag taa
//
