LOCUS       sequence-465389-        2853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells
            with localization in Cajal bodies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2853)
  TITLE     Rivera-Fuentes plasmids March 2025
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Grx1-roGFP2-Coilin
FEATURES             Location/Qualifiers
     source          1..2853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(388..390,391..1101)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(430..451)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(691..710)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1038..1059
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggctcaag agtttgtgaa ctgcaaaatc cagcctggga aggtggttgt gttcatcaag
       61 cccacctgcc cgtactgcag gagggcccaa gagatcctca gtcaattgcc catcaaacaa
      121 gggcttctgg aatttgtcga tatcacagcc accaaccaca ctaacgagat tcaagattat
      181 ttgcaacagc tcacgggagc aagaacggtg cctcgagtct ttattggtaa agattgtata
      241 ggcggatgca gtgatctagt ctctttgcaa cagagtgggg aactgctgac gcggctaaag
      301 cagattggag ctctgcagac tgaggaggag gccggcgaga agatcaagga gctggagaag
      361 aaggccagga agctgcccga ggaattcgtg agcaagggcg aggagctgtt caccggggtg
      421 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      481 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctc caccaccggc
      541 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      601 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      661 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      721 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      781 gaggacggca acatcctggg gcacaagctg gagtacaact acaactgcca caacgtctat
      841 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      901 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      961 cccgtgctgc tgcccgacaa ccactacctg agcacctgct ccgccctgag caaagacccc
     1021 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1081 ggcatggacg agctgtacaa gtactcagat ccaccaagca agatggcagc ttccgagacg
     1141 gttaggctac ggcttcaatt tgattacccg ccgccagcta ccccgcactg tacggccttc
     1201 tggcttctgg tcgacttgaa cagatgccga gtcgtcacag atctcattag tctcatccgc
     1261 cagcgcttcg gcttcagttc tggggccttc ctaggcctct acctggaggg ggggctcttg
     1321 ccccccgccg agagcgcgcg ccttgtgaga gacaacgact gcctcagagt taaattagaa
     1381 gagagaggag ttgctgagaa ttctgtagtc atcagtaatg gtgacattaa tttatctctt
     1441 agaaaagcaa agaagcgggc atttcagtta gaggagggtg aagaaactga accagattgc
     1501 aaatattcaa agaagcattg gaagagtcga gagaacaata acaataatga gaaggtcttg
     1561 gatctggaac caaaagctgt cacagatcag actgtcagca aaaaaaacaa gagaaaaaat
     1621 aaagcaacct gtggcacagt gggtgatgat aacgaagagg ccaaaagaaa atcaccaaag
     1681 aaaaaggaga aatgtgaata taaaaaaaag gctaagaatc ccaagtctcc gaaagtacag
     1741 gcagtgaaag actgggccaa tcagagatgt agttctccaa aaggttctgc tagaaacagc
     1801 cttgttaaag ccaaaaggaa aggtagtgta agcgtttgct caaaagagag tcccagttcc
     1861 tcctcggagt ctgagtcttg tgatgaatct atcagtgatg gtcccagcaa agtcactttg
     1921 gaggccagaa attcctcaga gaaattacca actgagttat caaaggaaga accctctacc
     1981 aaaaatacaa ctgcagacaa actggctata aaacttggct ttagccttac ccccagcaag
     2041 ggcaagacct ctggaacaac atcttccagt tcagactcta gtgcagagtc agacgaccaa
     2101 tgcttgatgt catcgagcac cccggagtgt gctgcgggtt tcttaaagac agtaggcctt
     2161 tttgcaggaa gaggtcgtcc aggcccaggg ctgtcatcac agactgcagg tgctgctgga
     2221 tggaggcgtt ctggctcaaa tggtggtgga caggctcctg gtgcttctcc cagtgtgtct
     2281 ctccctgcta gtttaggaag aggatggggt agagaagaga accttttttc ttggaaggga
     2341 gctaagggac ggggcatgcg ggggagaggt cgaggacgag ggcatcctgt ttcctgtgtt
     2401 gtaaatagaa gcactgacaa ccagaggcaa cagcaattaa atgacgtggt aaaaaattca
     2461 tctactatta tccagaatcc agtagagaca cccaagaagg actatagtct gttaccactg
     2521 ttagcagctg cccctcaagt tggagaaaag attgcattta agcttttgga gctaacatcc
     2581 agttactctc ctgatgtctc tgactacgag gaaggaagaa tattaagcca caatccagag
     2641 acccagcaag tagatataga aattctttca tccttacctg ccttgagaga acctgggaaa
     2701 tttgatttag tttatcacaa tgaaaatgga gctgaggtag tggagtacgc tgtgacacag
     2761 gagagcaaga tcactgtatt ttggaaagag ttgattgacc caagactgat tattgaatct
     2821 ccaagtaaca catcaagtac agaacctgcc taa
//
