LOCUS       sequence-465391-        2976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells
            with localization in processing bodies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2976)
  TITLE     Rivera-Fuentes plasmids March 2025
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Grx1-roGFP2-Dcp1b
FEATURES             Location/Qualifiers
     source          1..2976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(388..390,391..1101)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(430..451)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(691..710)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1038..1059
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggctcaag agtttgtgaa ctgcaaaatc cagcctggga aggtggttgt gttcatcaag
       61 cccacctgcc cgtactgcag gagggcccaa gagatcctca gtcaattgcc catcaaacaa
      121 gggcttctgg aatttgtcga tatcacagcc accaaccaca ctaacgagat tcaagattat
      181 ttgcaacagc tcacgggagc aagaacggtg cctcgagtct ttattggtaa agattgtata
      241 ggcggatgca gtgatctagt ctctttgcaa cagagtgggg aactgctgac gcggctaaag
      301 cagattggag ctctgcagac tgaggaggag gccggcgaga agatcaagga gctggagaag
      361 aaggccagga agctgcccga ggaattcgtg agcaagggcg aggagctgtt caccggggtg
      421 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      481 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctc caccaccggc
      541 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      601 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      661 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      721 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      781 gaggacggca acatcctggg gcacaagctg gagtacaact acaactgcca caacgtctat
      841 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      901 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      961 cccgtgctgc tgcccgacaa ccactacctg agcacctgct ccgccctgag caaagacccc
     1021 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1081 ggcatggacg agctgtacaa gtccggactc agatctcgag ctgcagccgt ggcggcaggc
     1141 ggcctggtgg gaaaggggcg cgacatcagc ctagcggccc tgcagcgcca cgacccctat
     1201 atcaaccgca tcgtggacgt ggccagccag gtggctctgt acaccttcgg ccatcgggcc
     1261 aacgagtggg agaaaactga tgtggaagga accttatttg tttatacaag gtctgcttct
     1321 ccaaagcatg gattcaccat tatgaatagg ctgagcatgg aaaataggac agaacctatt
     1381 actaaagact tggatttcca actccaggac cctttccttc tctacagaaa tgccagattg
     1441 tccatctatg gaatttggtt ttatgataag gaagaatgcc aaagaattgc agagcttatg
     1501 aaaaacctaa ctcagtatga acagttgaaa gcccatcagg gaactggagc aggaatttca
     1561 ccagtgatcc tcaattcagg agagggcaaa gaagtagaca ttttacgaat gctcatcaag
     1621 gccaaagacg aatacacaaa gtgtaaaacc tgttctgagc caaaaaagat aaccagttcc
     1681 tctgccatct atgacaatcc aaatctcatc aaaccaattc cagtgaaacc cagtgaaaac
     1741 cagcaacagc gtatacctca gcccaaccag accttagacc ctgaacccca acacttatcc
     1801 ttgacagctc tgtttgggaa gcaggacaaa gctacatgtc aggaaactgt ggagcctccg
     1861 cagactctcc accagcagca gcagcagcag cagcagcagc agcaagagaa gcttccaatt
     1921 aggcaggggg ttgtacgctc cctgtcctat gaggaaccca gaagacactc accccccatt
     1981 gagaagcagc tctgtccagc cattcagaaa ctcatggtca ggagcgcaga cctccaccca
     2041 ttgtcagagc tgcctgaaaa ccggccttgt gaaaatggca gtacccattc tgcgggagaa
     2101 ttttttacag gacctgtcca gccagggtct cctcacaaca ttggaacttc tcgtggtgta
     2161 caaaatgctt ccagaactca gaacctgttc gagaaacttc agagtacccc aggggcagca
     2221 aacaagtgtg accctagtac accagcacct gccagctcag ctgccctgaa ccgcagcaga
     2281 gctcccactt ctgtcacccc tgtggctcca ggaaagggtc tggctcagcc accacaggcc
     2341 tatttcaatg gctcccttcc acctcagaca gtaggacatc aggctcatgg aagagaacag
     2401 tccacactcc caagacaaac actcgccatc tctggtagtc agactggcag ctctggagtg
     2461 atctcccctc aagagttact gaagaagctt cagattgtac agcaggagca gcagctgcat
     2521 gcctctaacc ggccagcctt ggccgctaag tttcctgtgc tcgctcagag ctctggaaca
     2581 gggaaaccct tggaatcctg gatcaacaag acacccaaca cagaacagca gactcctctt
     2641 ttccaggtca tctcacctca gcgcatccct gctacagcag ctccgtctct gcttatgtcc
     2701 cccatggtgt tcgcacaacc cacctccgtc ccgccaaagg aaagggagag cggcctcttg
     2761 cctgtgggag gccaggagcc acctgttgct gccaccagcc tcctcctgcc catacagagc
     2821 ccggagccct ccgtgatcac cagcagccca ctcaccaagc tccagctcca ggaggcactg
     2881 ctgtacctca ttcagaatga tgacaacttc ttaaatataa tctatgaagc ctatctcttc
     2941 agcatgactc aagcagccat gaaaaagact atgtga
//
