LOCUS       sequence-465393-        2517 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells
            with localization in stress granules..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2517)
  TITLE     Rivera-Fuentes plasmids March 2025
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: G3BP1-Grx1-roGFP2
FEATURES             Location/Qualifiers
     source          1..2517
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1801..1803,1804..2517)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1843..1864)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2104..2123)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2451..2472
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgatgg agaagcctag tcccctgctg gtcgggcggg aatttgtgag acagtattac
       61 acactgctga accaggcccc agacatgctg catagatttt atggaaagaa ctcttcttat
      121 gtccatgggg gattggattc aaatggaaag ccagcagatg cagtctacgg acagaaagaa
      181 atccacagga aagtgatgtc acaaaacttc accaactgcc acaccaagat tcgccatgtt
      241 gatgctcatg ccacgctaaa tgatggtgtg gtagtccagg tgatggggct tctctctaac
      301 aacaaccagg ctttgaggag attcatgcaa acgtttgtcc ttgctcctga ggggtctgtt
      361 gcaaataaat tctatgttca caatgatatc ttcagatacc aagatgaggt ctttggtggg
      421 tttgtcactg agcctcagga ggagtctgaa gaagaagtag aggaacctga agaaagacag
      481 caaacacctg aggtggtacc tgatgattct ggaactttct atgatcaggc agttgtcagt
      541 aatgacatgg aagaacattt agaggagcct gttgctgaac cagagcctga tcctgaacca
      601 gaaccagaac aagaacctgt atctgaaatc caagaggaaa agcctgagcc agtattagaa
      661 gaaactgccc ctgaggatgc tcagaagagt tcttctccag cacctgcaga catagctcag
      721 acagtacagg aagacttgag gacattttct tgggcatctg tgaccagtaa gaatcttcca
      781 cccagtggag ctgttccagt tactgggata ccacctcatg ttgttaaagt accagcttca
      841 cagccccgtc cagagtctaa gcctgaatct cagattccac cacaaagacc tcagcgggat
      901 caaagagtgc gagaacaacg aataaatatt cctccccaaa ggggacccag accaatccgt
      961 gaggctggtg agcaaggtga cattgaaccc cgaagaatgg tgagacaccc tgacagtcac
     1021 caactcttca ttggcaacct gcctcatgaa gtggacaaat cagagcttaa agatttcttt
     1081 caaagttatg gaaacgtggt ggagttgcgc attaacagtg gtgggaaatt acccaatttt
     1141 ggttttgttg tgtttgatga ttctgagcct gttcagaaag tccttagcaa caggcccatc
     1201 atgttcagag gtgaggtccg tctgaatgtc gaagagaaga agactcgagc tgccagggaa
     1261 ggcgaccgac gagataatcg ccttcgggga cctggaggcc ctcgaggtgg gctgggtggt
     1321 ggaatgagag gccctccccg tggaggcatg gtgcagaaac caggatttgg agtgggaagg
     1381 gggcttgcgc cacggcagca gtcgaccggc agcatggctc aagagtttgt gaactgcaaa
     1441 atccagcctg ggaaggtggt tgtgttcatc aagcccacct gcccgtactg caggagggcc
     1501 caagagatcc tcagtcaatt gcccatcaaa caagggcttc tggaatttgt cgatatcaca
     1561 gccaccaacc acactaacga gattcaagat tatttgcaac agctcacggg agcaagaacg
     1621 gtgcctcgag tctttattgg taaagattgt ataggcggat gcagtgatct agtctctttg
     1681 caacagagtg gggaactgct gacgcggcta aagcagattg gagctctgca gactgaggag
     1741 gaggccggcg agaagatcaa ggagctggag aagaaggcca ggaagctgcc cgaggaattc
     1801 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1861 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1921 aagctgaccc tgaagttcat ctccaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1981 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     2041 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2101 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2161 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2221 ctggagtaca actacaactg ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     2281 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2341 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2401 ctgagcacct gctccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2461 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaa
//
