LOCUS       sequence-465395-        2673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells
            with localization in paraspeckles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2673)
  TITLE     Rivera-Fuentes plasmids March 2025
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Grx1-roGFP2-PSPC1
FEATURES             Location/Qualifiers
     source          1..2673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(388..390,391..1101)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(430..451)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(691..710)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1038..1059
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2453..2464
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggctcaag agtttgtgaa ctgcaaaatc cagcctggga aggtggttgt gttcatcaag
       61 cccacctgcc cgtactgcag gagggcccaa gagatcctca gtcaattgcc catcaaacaa
      121 gggcttctgg aatttgtcga tatcacagcc accaaccaca ctaacgagat tcaagattat
      181 ttgcaacagc tcacgggagc aagaacggtg cctcgagtct ttattggtaa agattgtata
      241 ggcggatgca gtgatctagt ctctttgcaa cagagtgggg aactgctgac gcggctaaag
      301 cagattggag ctctgcagac tgaggaggag gccggcgaga agatcaagga gctggagaag
      361 aaggccagga agctgcccga ggaattcgtg agcaagggcg aggagctgtt caccggggtg
      421 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      481 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctc caccaccggc
      541 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      601 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      661 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      721 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      781 gaggacggca acatcctggg gcacaagctg gagtacaact acaactgcca caacgtctat
      841 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      901 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      961 cccgtgctgc tgcccgacaa ccactacctg agcacctgct ccgccctgag caaagacccc
     1021 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1081 ggcatggacg agctgtacaa gatgatgtta agaggaaacc tgaagcaagt gcgcattgag
     1141 aaaaacccgg cccgccttcg cgccctggag tccgcggtgg gcgagagcga gccggcggcc
     1201 gcggcagcca tggcgctcgc tcttgccggg gagccggcac cgcccgcgcc cgcgcctcca
     1261 gaggaccacc cggacgagga gatggggttc actatcgaca tcaagagttt cctcaagccg
     1321 ggcgagaaga cgtacacgca gcgctgccgc ctcttcgtgg gaaatctgcc caccgacatc
     1381 acggaggagg acttcaagag gctcttcgaa cgctatggcg agcccagcga agtcttcatc
     1441 aaccgggacc gtggcttcgg cttcatccgc ttggaatcca gaaccctggc tgaaattgca
     1501 aaagcagagc tggacggcac cattctcaag agcagacctc tacggattcg cttcgctaca
     1561 catggagcag ccttgactgt caagaacctt tctccagttg tttccaatga gctgctagag
     1621 caagcatttt ctcagtttgg tccagtagag aaagctgttg tggttgtgga tgatcgcggt
     1681 agagctacag gaaaaggttt tgtagagttt gcagcaaaac ctcctgcacg aaaggctctg
     1741 gaaagatgtg gtgatggggc attcttgcta acaacgaccc ctcgtccagt cattgtggaa
     1801 cccatggagc agtttgatga tgaagatggc ttgccagaga agctgatgca gaaaactcaa
     1861 caatatcata aggaaagaga acaaccacca cgttttgctc aacctgggac atttgaattt
     1921 gagtatgcat ctcgatggaa ggctcttgat gaaatggaaa agcagcagcg tgagcaggtt
     1981 gatagaaaca tcagagaagc caaagagaaa ctggaggcag aaatggaagc agctaggcat
     2041 gaacaccaat taatgctaat gaggcaagat ctaatgaggc gtcaagaaga actcagacgc
     2101 ttggaagaac tcagaaacca agagttgcaa aaacggaagc aaatacaact aagacatgaa
     2161 gaggagcatc ggcggcgtga ggaagaaatg atccgacaca gagaacagga ggaactgagg
     2221 cgacagcaag agggctttaa gccaaactac atggaaaata gagaacagga aatgagaatg
     2281 ggtgatatgg gtccccgtgg agcaataaac atgggagatg cgtttagccc agcccctgct
     2341 ggtaaccaag gtcctcctcc aatgatgggt atgaatatga acaacagagc aactatacct
     2401 ggcccaccaa tgggtcctgg tcctgccatg ggaccagaag gagccgcaaa tatgggaact
     2461 ccaatgatgc cagataatgg agcagtgcac aatgacagat ttcctcaagg accaccatct
     2521 cagatgggtt cacctatggg gagtagaaca ggttctgaaa cccctcaagc accaatgagt
     2581 ggtgtaggtc ctgtgagtgg tggtcctggt ggctttggta gaggaagtca agggggcaac
     2641 tttgaaggcc ctaataagcg tcgtagatat taa
//
