LOCUS       sequence-465397-        1497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells
            with localization in the nucleus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1497)
  TITLE     Rivera-Fuentes plasmids March 2025
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: H2B-Grx1-roGFP2
FEATURES             Location/Qualifiers
     source          1..1497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..378
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSQAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             join(781..783,784..1497)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(823..844)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1084..1103)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1431..1452
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact
       61 aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc
      121 tatgtgtaca aggttctgaa gcaggtccac cctgacaccg gcatttcgtc ccaggccatg
      181 ggcatcatga attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc
      241 ctggcgcatt acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc
      301 ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc
      361 aagtacacta gcgctaagca gtcgaccggc agcatggctc aagagtttgt gaactgcaaa
      421 atccagcctg ggaaggtggt tgtgttcatc aagcccacct gcccgtactg caggagggcc
      481 caagagatcc tcagtcaatt gcccatcaaa caagggcttc tggaatttgt cgatatcaca
      541 gccaccaacc acactaacga gattcaagat tatttgcaac agctcacggg agcaagaacg
      601 gtgcctcgag tctttattgg taaagattgt ataggcggat gcagtgatct agtctctttg
      661 caacagagtg gggaactgct gacgcggcta aagcagattg gagctctgca gactgaggag
      721 gaggccggcg agaagatcaa ggagctggag aagaaggcca ggaagctgcc cgaggaattc
      781 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      841 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      901 aagctgaccc tgaagttcat ctccaccacc ggcaagctgc ccgtgccctg gcccaccctc
      961 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     1021 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1081 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1141 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1201 ctggagtaca actacaactg ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1261 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1321 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1381 ctgagcacct gctccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1441 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaa
//
