LOCUS       sequence-465399-        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells
            with localization in the cytoplasm..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  TITLE     Rivera-Fuentes plasmids March 2025
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NES-Grx1-roGFP2
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(451..453,454..1167)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(493..514)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(754..773)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1101..1122
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggctctgc aaaagaagct ggaagaactg gaactggatg aaggaggaca gtcgaccggc
       61 agcatggctc aagagtttgt gaactgcaaa atccagcctg ggaaggtggt tgtgttcatc
      121 aagcccacct gcccgtactg caggagggcc caagagatcc tcagtcaatt gcccatcaaa
      181 caagggcttc tggaatttgt cgatatcaca gccaccaacc acactaacga gattcaagat
      241 tatttgcaac agctcacggg agcaagaacg gtgcctcgag tctttattgg taaagattgt
      301 ataggcggat gcagtgatct agtctctttg caacagagtg gggaactgct gacgcggcta
      361 aagcagattg gagctctgca gactgaggag gaggccggcg agaagatcaa ggagctggag
      421 aagaaggcca ggaagctgcc cgaggaattc gtgagcaagg gcgaggagct gttcaccggg
      481 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      541 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctccaccacc
      601 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      661 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      721 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      781 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      841 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaactg ccacaacgtc
      901 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      961 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1021 ggccccgtgc tgctgcccga caaccactac ctgagcacct gctccgccct gagcaaagac
     1081 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1141 ctcggcatgg acgagctgta caagtaa
//
