LOCUS       sequence-465426-        9206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGR1 - Early growth response factor gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE45
FEATURES             Location/Qualifiers
     source          1..9206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(227..246)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     574..595
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             712..945
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1017..1138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1054..1073)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1108..1127
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1178..1313
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1333..1352)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1334..1352)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1362..1379)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1362..1378)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1371..1393)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1520..1975
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1607..1626)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1817..1838
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2001..2105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2106..2174,2175..2966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2324..2343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3137..3725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3626..3645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3879..3896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4013..4034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4049..4054,4055..4072,4073..4079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4087..4103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4092..4114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4111..4127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4111..4127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4146..4166
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4148..4166
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4194..4420
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4421..4601
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4648..4773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5266..5499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5684..5728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5995..6112
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6229..6260
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      6287..6296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6293..7942
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             7946..8065
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    8157..8745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8210..8230)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8628..8639)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8760..9076
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8927..9062
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(9127..9129,9130..9132,9133..9206,1..640)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9172..9193)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
       61 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta
      121 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      181 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      241 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      301 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
      361 cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa
      421 gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
      481 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
      541 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      601 cgccgggatc actctcggca tggacgagct gtacaagtaa ggtaccttta agaccaatga
      661 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
      721 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
      781 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      841 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      901 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
      961 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     1021 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     1081 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     1141 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     1201 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     1261 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     1321 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     1381 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     1441 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     1501 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     1561 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     1621 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     1681 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     1741 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     1801 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     1861 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     1921 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     1981 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2041 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2101 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2161 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2221 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2281 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2341 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2401 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2461 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2521 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2581 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2641 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2701 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2761 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2821 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2881 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2941 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3001 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3061 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3121 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3181 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3241 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3301 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3361 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3421 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3481 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3541 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3601 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3661 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3721 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3781 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3841 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3901 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3961 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     4021 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4081 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     4141 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     4201 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     4261 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     4321 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     4381 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     4441 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4501 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4561 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     4621 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     4681 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     4741 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     4801 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     4861 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     4921 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     4981 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     5041 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     5101 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     5161 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     5221 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     5281 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     5341 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     5401 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     5461 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     5521 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     5581 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     5641 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     5701 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     5761 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     5821 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     5881 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     5941 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     6001 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     6061 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatcg
     6121 caccagcgtg tggatccgcc cccgcgcgcc cccgcgcgcc cccgcgcgcc cccgcgcgcc
     6181 cccgcggatc cgagaacaga tctggcctcg gcggccaagc ttagacacta gagggtatat
     6241 aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca ccatggaaga
     6301 tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg
     6361 cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac
     6421 cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct
     6481 ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga
     6541 gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc
     6601 cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc
     6661 caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct
     6721 accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag
     6781 catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc
     6841 cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg
     6901 attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg
     6961 cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt
     7021 tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt
     7081 gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca
     7141 atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa
     7201 gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt
     7261 aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac
     7321 agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg
     7381 caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg
     7441 tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa
     7501 caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat
     7561 cgcctactgg gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa
     7621 atacaagggc taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa
     7681 catcttcgac gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc
     7741 agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc
     7801 cagccaggtt acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc
     7861 taaaggactg accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa
     7921 gaagggcggc aagatcgccg tgaattctca cggcttccct cccgaggtgg aggagcaggc
     7981 cgccggcacc ctgcccatga gctgcgccca ggagagcggc atggatagac accctgctgc
     8041 ttgcgccagc gccaggatca acgtctaagg ccgcgactct agaacgcgtc actgcggctt
     8101 gagggcgtgg atcgtcgact acagacatca gcgcgtaccg gttagtaatg atcgacaatc
     8161 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     8221 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     8281 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     8341 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     8401 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     8461 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     8521 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     8581 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     8641 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     8701 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggc gatggtaccg
     8761 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8821 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8881 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     8941 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     9001 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9061 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     9121 cctagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     9181 ctggacggcg acgtaaacgg ccacaa
//
