LOCUS       sequence-465443-       11322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Salpingoeca helianthica chimeric Pchi in mammalian cells, 
            for protein overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11322)
  AUTHORS   Gao Y, Tan DS, Girbig M, Hu H, Zhou X, Xie Q, Yeung SW, Lee KS, Ho 
            SY, Cojocaru V, Yan J, Hochberg GKA, de Mendoza A, Jauch R
  TITLE     The emergence of Sox and POU transcription factors predates the 
            origins of animal stem cells.
  JOURNAL   Nat Commun. 2024 Nov 14;15(1):9868. doi: 10.1038/s41467-024-54152-x.
  PUBMED    39543096
REFERENCE   2  (bases 1 to 11322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-11-14"; volume: "15(1):9868. doi"; pages: " 
            10.1038/s41467-024-54152-x"
COMMENT     Sequence Label: pLVTHM-Chim-Pchi-3xFLAG
FEATURES             Location/Qualifiers
     source          1..11322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2147..3325
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2378..3316
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3273..3293
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3373..3490
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4495..4560
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4578..5166
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4631..4651)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5049..5060)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5334..5352,5353..5375,5376..5394,5395..5417,5418..5436,
                     5437..5459,5460..5478,5479..5501,5502..5520,5521..5543,
                     5544..5562,5563..5585,5586..5604)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5633..5847
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     5768..5787
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(5885..5918)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5938..6118
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6143..6161)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6144..6161)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6451..6470)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6684..6703)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6803..6825
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6863..6881)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6949..7053
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7054..7122,7123..7914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7272..7291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8085..8673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8574..8593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8827..8844
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8914..8934)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8919..9248
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9099..9234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10382..10447
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10577..10597
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11022..11156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11059..11078)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11113..11132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgcga tgtcgacgat aagctttgca aagatggata aagttttaaa cagagaggaa
     2101 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2161 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2221 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2281 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2341 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2401 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     2461 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     2521 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     2581 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     2641 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     2701 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     2761 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     2821 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     2881 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     2941 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3001 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3061 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3121 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3181 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3241 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3301 ttttcttcca tttcaggtgt cgtgaggaat ttcgacattt aaatttaatt aatctcgacg
     3361 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     3421 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     3481 tcaaaatttt atcgatcacg agactagcct cgaggtttaa acatgtacaa catgatggaa
     3541 acagagctga agcctccagg acctcaacaa gccagcggtg gaggtggcgg cggtgggaac
     3601 gctaccgccg ctgctaccgg cggtaatcag aagaacagcc catcccacat caaaaggcca
     3661 atgaactctt ttatggtgtg ggcccaaatg atgaggaagg agctgaccca gaagcaccct
     3721 gacctccaca atgctgagat cagcaaacgc ctgggagaga tgtggaaaga actccctgaa
     3781 agcgacaaac aaccttacgt ggagcgggct caggctctga agcaactgca tgagcaggag
     3841 tttccctact acaaatatcg cccaagaagg aaaagctcta ctctgatgaa gaaagataag
     3901 tacacactgc ccggagggct cctcgcacca ggtggcaaca gtatggcctc tggagttgga
     3961 gtgggcgctg gcctgggcgc aggcgttaac cagcgcatgg actcttacgc ccacatgaac
     4021 ggatggtcca acggtagtta ctccatgatg caggagcagc tcggctaccc tcagcatccc
     4081 gggctcaatg cccatggagc cgcccagatg cagccaatgc ataggtacga tgttagcgct
     4141 ctgcagtaca atagcatgac cagcagccag acttacatga atggtagccc tacttattcc
     4201 atgagctata gtcaacaggg aacacccgga atggctctgg gaagcatggg aagcgtggtc
     4261 aagagcgagg caagcagtag ccctcctgtg gtcacctcca gctcccattc ccgcgcacca
     4321 tgccaggctg gtgacctcag agacatgatc tccatgtatc tccctggcgc cgaggtgcca
     4381 gaaccagccg ctccaagcag gctgcacatg gctcagcact atcagagtgg ccctgttccc
     4441 ggaaccgcca tcaacggcac tctgcctctg tctcacatga cgcgtggcag cggcgactac
     4501 aaggaccacg acggcgacta caaggaccac gacatcgact acaaggacga cgacgacaag
     4561 tagactagtc atatgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4621 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4681 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4741 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4801 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4861 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4921 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     4981 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5041 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5101 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5161 ccccgcatcg gtacgtatgg ccaggtacct ttaagaccaa tgacttacaa ggcagctgta
     5221 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5281 agacaagatg ggatcaattc accatgctag tggatccccc tcgagtttac cactccctat
     5341 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     5401 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     5461 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     5521 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     5581 accactccct atcagtgata gagaaaagtg aaagtcgggg ctgcaggaat tcgaacgctg
     5641 acgtcatcaa cccgctccaa ggaatcgcgg gcccagtgtc actaggcggg aacacccagc
     5701 gcgcgtgcgc cctggcagga agatggctgt gagggacagg ggagtggcgc cctgcaatat
     5761 ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt tggatttggg
     5821 aatcttataa gttctgtatg agaccacgcg gcgtctccaa gcttatcgat accgtcgcat
     5881 gggaataact tcgtatagca tacattatac gaagttatgc tgctttttgc ttgtactggg
     5941 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6001 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6061 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
     6121 atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata
     6181 aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag
     6241 catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt
     6301 tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg
     6361 aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca
     6421 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     6481 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     6541 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     6601 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     6661 agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     6721 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     6781 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     6841 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     6901 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6961 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7021 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7081 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7141 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7201 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7261 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7321 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7381 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7441 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7501 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7561 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7621 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7681 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7741 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7801 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7861 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7921 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7981 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8041 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8101 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8161 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8221 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8281 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8341 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8401 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8461 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8521 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8581 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8641 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8701 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8761 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8821 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8881 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
     8941 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9001 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9061 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     9121 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     9181 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     9241 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
     9301 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
     9361 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
     9421 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
     9481 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
     9541 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
     9601 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
     9661 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
     9721 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
     9781 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
     9841 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
     9901 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
     9961 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
    10021 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
    10081 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
    10141 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
    10201 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
    10261 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
    10321 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
    10381 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
    10441 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
    10501 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
    10561 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
    10621 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
    10681 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
    10741 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
    10801 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
    10861 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
    10921 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    10981 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    11041 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    11101 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    11161 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    11221 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11281 aaagaaattg tatttgttaa atatgtacta caaacttagt ag
//
