LOCUS       sequence-465451-        8508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generates MCM2 dTAG C ternimal knock-in cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8508)
  TITLE     Chen knock-in cell line plasmids
REFERENCE   2  (bases 1 to 8508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pX459_MCM2_sgRNA
FEATURES             Location/Qualifiers
     source          1..8508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             906..953
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(981..998)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    987..1194
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1203..1343
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1203..1332
                     /label=AAV2 ITR
     rep_origin      1418..1873
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1505..1524)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1715..1736
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1890..1909)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2009..2031
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2069..2087)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2155..2259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2260..2328,2329..3120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2478..2497)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3291..3879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3780..3799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3941..4189
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3941..3961
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4112..4131
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4211..4286
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        4383..4668
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        4670..4947
                     /label=chicken beta-actin promoter
     intron          4948..5175
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      5187..5196
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5196..5261
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5268..5288
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5313..8508,1..905)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(5313..8508,1..905)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc
       61 tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca
      121 aagagtctat cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg
      181 accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg
      241 ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca
      301 ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct
      361 acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg
      421 aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg
      481 ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg
      541 gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg
      601 acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc
      661 tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg
      721 agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact
      781 ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc
      841 tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag
      901 gcgacaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aagtaagaat
      961 tcctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     1021 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     1081 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     1141 ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg gggagcggcc
     1201 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     1261 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     1321 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     1381 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     1441 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     1501 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1561 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1621 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1681 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1741 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     1801 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     1861 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1921 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     1981 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     2041 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     2101 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2161 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2221 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2281 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2341 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2401 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2461 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2521 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2581 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2641 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2701 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2761 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2821 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2881 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2941 tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg
     3001 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3061 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3121 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3181 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3241 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3301 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3361 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3421 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3481 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3541 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3601 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3661 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3721 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3781 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3841 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3901 ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat ttcccatgat
     3961 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
     4021 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     4081 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     4141 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gaggccctat
     4201 gccatccata gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     4261 ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga gctagaaata gcaagttaaa
     4321 ataaggctag tccgttttta gcgcgtgcgc caattctgca gacaaatggc tctagaggta
     4381 cccgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     4441 ttgacgtcaa tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     4501 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     4561 gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc agtacatgac cttatgggac
     4621 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     4681 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
     4741 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc gcgcgcgcca
     4801 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
     4861 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
     4921 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
     4981 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
     5041 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agctgagcaa gaggtaaggg
     5101 tttaagggat ggttggttgg tggggtatta atgtttaatt acctggagca cctgcctgaa
     5161 atcacttttt ttcaggttgg accggtgcca ccatggacta taaggaccac gacggagact
     5221 acaaggatca tgatattgat tacaaagacg atgacgataa gatggcccca aagaagaagc
     5281 ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa gtacagcatc ggcctggaca
     5341 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
     5401 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
     5461 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
     5521 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
     5581 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
     5641 agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
     5701 agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc
     5761 tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg
     5821 agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga
     5881 cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca
     5941 tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg
     6001 gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca
     6061 acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct
     6121 acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc
     6181 tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg
     6241 agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg
     6301 acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt
     6361 tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag
     6421 agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg
     6481 tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc
     6541 cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc
     6601 cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact
     6661 acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg
     6721 aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct
     6781 tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc
     6841 acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga
     6901 ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc
     6961 tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga
     7021 aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc
     7081 tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg
     7141 aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga
     7201 tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc
     7261 tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc
     7321 gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca
     7381 gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag
     7441 cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc
     7501 ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga
     7561 tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc
     7621 agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc
     7681 tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc
     7741 tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca
     7801 accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact
     7861 ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc
     7921 cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc
     7981 tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac
     8041 tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg
     8101 tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc
     8161 gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc
     8221 agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg
     8281 ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg
     8341 gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca
     8401 aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta
     8461 ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacg
//
