LOCUS       sequence-465475-       12353 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ISYmu1 PDS3 gRNA2 TRV2 Architecture_A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12353)
  AUTHORS   Weiss T, Kamalu M, Shi H, Li Z, Amerasekera J, Zhong Z, Adler BA, 
            Song MM, Vohra K, Wirnowski G, Chitkara S, Ambrose C, Steinmetz N, 
            Sridharan A, Sahagun D, Banfield JF, Doudna JA, Jacobsen SE
  TITLE     Viral delivery of an RNA-guided genome editor for transgene-free 
            germline editing in Arabidopsis.
  JOURNAL   Nat Plants. 2025 Apr 22. doi: 10.1038/s41477-025-01989-9.
  PUBMED    40263581
REFERENCE   2  (bases 1 to 12353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2025 Apr 22. doi: 10.1038/s41477-025-01989-9."
COMMENT     Sequence Label: pTW2065
FEATURES             Location/Qualifiers
     source          1..12353
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2349..2396
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             2403..2423
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4283..4300
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4335..4587
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4609..4633
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5934..6563
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6992..8065
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8131..8325
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8561..8583)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8669..8809
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8724..8743
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8824..8841)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8995..9583)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9075..9094)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11008..11802)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12227..12251
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gcacacaagg ttaaaaacgc tgtagtaata catgcgcaag aacaggctga
     2161 gcatcttgtt ctggggtttc acactatctt tagagaaagt gttaagttaa ttaagttatc
     2221 ttaattaaga gcataattat actgatttgt ctctcgttga tagagtctat cattctgtta
     2281 ttaaaaattt gacaactcgg tttgctgacc tactggtcac tgtatcactt acccgagtta
     2341 acgagatgga ttacaaggat gatgatgata aggattacaa ggatgatgat gataagatgg
     2401 ctccaaagaa gaagagaaag gttggaatcc acggagttcc agctgctatg ctgcagcata
     2461 aagcctatga ataccgtatc tatccagata agaagcagga aacactgatt gccaagacaa
     2521 taggcagttc aaggtatgta tacaaccatt tcctggaact ttggaacaag gaatatgagg
     2581 agaccggaaa aggtctgact tattacgcat gctccaaact tctgacaaag cttaagaggg
     2641 acccggaaac agtatggctt tgtgaagtgg ataagttctc gctccagaat tcgcttcgta
     2701 acctgtcgga cgccttctcc cgctttttca aaggacagaa cgagcatccc cagttcaaga
     2761 gcaagaagag cccgaggcaa agctacacga cacaatatac gaacaacaac atcgcagttt
     2821 ccggaaattg tctgaagctg cctaagctgg gccttgtcaa gtttgcagac agtagggaga
     2881 tgaaaggccg tatactgaat gccacagtac gaaggaaatc gagcgggaaa ttcttcgtat
     2941 cgatcctatg cgaagaggag atctgtgaat tgccaaagac tgactcatct gtcggaattg
     3001 acctcggaat cattgacttt gcggttatgt cagacggaag caggcatgac aacaatcatt
     3061 ttaccaggca aatggaagaa aggcttagac gggagcagcg aaagcttgca aggcgtgcac
     3121 ttgctgcgga gaaaaggggc atttcccttt ctgaagccag gaactatcag aagcaaaggc
     3181 ggaaggtagc gagactttat gaaaaggttg caaaccagcg aaaagaatat cttaacaaac
     3241 tcagtacaga gatagtcaaa aaccacgata tcatctgtat cgaggacctt aacgttaagg
     3301 gcatgatgcg caatcataaa ctggcaaaaa gcatctctga tgtatcatgg acgagccttg
     3361 tatcgaaact gcagtacaag gcttcctggt atggaaaaga agtcatcagg ataagcagat
     3421 ggtttccgtc aagtcagata tgctcagagt gcggccacaa ggacaggaag aagcctctcc
     3481 atgtaaggga gtggacctgt cctgtttgtc atgcccacca tgaccgtgac gtcaatgcag
     3541 caagaaatat actggctgag ggactcagga taagagctct gactccgggg tcttagcaat
     3601 acagaagcaa acaggaaccg caggaattgc gggggtagct tggtaaacaa gagaaacctc
     3661 tgccggcaaa gaaataagcc ggtaagtatg ctctgttccc aagaatctcg tgactttagt
     3721 catgagagtt tcaaaaggca aattcgccgc ccgtagctca gttggttaga gcgttggtct
     3781 tatgagccga aggtcgcggg ttcgagcccc gccggaagca cccgggcatg tcccgaagac
     3841 attaaactac ggttctttaa gtagatccgt gtctgaagtt ttaggttcaa tttaaaccta
     3901 cgagattgac attctcgact gatcttgatt gatcggtaag tcttttgtaa tttaattttc
     3961 tttttgattt tattttaaat tgttatctgt ttctgtgtat agactgtttg agatcggcgt
     4021 ttggccgact cattgtctta ccatagggga acggactttg tttgtgttgt tattttattt
     4081 gtattttatt aaaattctca acgatctgaa aaagcctcgc ggctaagaga ttgttggggg
     4141 gtgagtaagt acttttaaag tgatgatggt tacaaaggca aaaggggtaa aacccctcgc
     4201 ctacgtaagc gttattacgc ccgtctgtac ttatatcagt acactgacga gtccctaaag
     4261 gacgaaacgg gagaacgcta gccaccacca ccaccaccac gtgtgaatta caggtgacca
     4321 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     4381 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     4441 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     4501 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     4561 gcgcggtgtc atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata
     4621 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     4681 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     4741 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     4801 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     4861 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     4921 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     4981 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     5041 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     5101 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     5161 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     5221 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     5281 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     5341 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     5401 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     5461 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     5521 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     5581 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     5641 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     5701 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     5761 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     5821 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     5881 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     5941 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     6001 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     6061 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     6121 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     6181 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     6241 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     6301 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     6361 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     6421 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     6481 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     6541 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     6601 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     6661 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     6721 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     6781 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     6841 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     6901 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg
     6961 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     7021 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     7081 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     7141 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     7201 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     7261 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     7321 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     7381 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     7441 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     7501 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     7561 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     7621 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     7681 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     7741 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     7801 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     7861 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     7921 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     7981 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     8041 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     8101 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
     8161 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     8221 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     8281 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     8341 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
     8401 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
     8461 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
     8521 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     8581 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     8641 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     8701 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     8761 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     8821 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8881 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8941 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9001 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9061 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9121 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9181 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9241 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9301 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9361 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9421 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9481 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9541 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9601 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggctga tgaatcccct
     9661 aatgattttt atcaaaatca ttaagttaag gtagatacac atcttgtcat atgatcaaat
     9721 ggtttcgcca aaaatcaata atcagacaac aaaatgtgcg aactcgatat tttacacgac
     9781 tctctttacc aattctgccc cgaattacac ttaaaacgac tcaacagctt aacgttggct
     9841 tgccacgcct tacttgactg taaaactctc actcttaccg aacttggccg taacctgcca
     9901 accaaagcga gaacaaaaca taacatcaaa cgaatcgacc gattgttagg taatcgtcac
     9961 ctccacaaag agcgactcgc tgtataccgt tggcatgcta gctttatctg ttcgggcaat
    10021 acgatgccca ttgtacttgt tgactggtct gatatccgtg agcaaaaacg gcttatggta
    10081 ttgcgagctt cagtcgcact acacggtcgt tctgttactc tttatgagaa agcgttcccg
    10141 ctttcagagc aatgttcaaa gaaagctcat gaccaatttc tagccgacct tgcgagcatt
    10201 ctaccgagta acaccacacc gctcattgtc agtgatgctg gctttaaagt gccatggtat
    10261 aaatccgttg agaagctggg ttggtactgg ttaagtcgag taagaggaaa agtacaatat
    10321 gcagacctag gagcggaaaa ctggaaacct atcagcaact tacatgatat gtcatctagt
    10381 cactcaaaga ctttaggcta taagaggctg actaaaagca atccaatctc atgccaaatt
    10441 ctattgtata aatctcgctc taaaggccga aaaaatcagc gctcgacacg gactcattgt
    10501 caccacccgt cacctaaaat ctactcagcg tcggcaaagg agccatggat tctagcaact
    10561 aacttacctg ttgaaattcg aacacccaaa caacttgtta atatctattc gaagcgaatg
    10621 cagattgaag aaaccttccg agacttgaaa agtcctgcct acggactagg cctacgccat
    10681 agccgaacga gcagctcaga gcgttttgat atcatgctgc taatcgccct gatgcttcaa
    10741 ctaacatgtt ggcttgcggg cgttcatgct cagaaacaag gttgggacaa gcacttccag
    10801 gctaacacag tcagaaatcg aaacgtactc tcaacagttc gcttaggcat ggaagttttg
    10861 cggcattctg gctacacaat aacaagggaa gactcactcg tggctgcaac cctgcttact
    10921 caaaatctat tcacacatgg ttacgttttg gggaaattat gaggggatct ctcagcgtta
    10981 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
    11041 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
    11101 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
    11161 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    11221 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    11281 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    11341 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    11401 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    11461 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    11521 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    11581 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    11641 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    11701 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    11761 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    11821 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    11881 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    11941 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
    12001 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
    12061 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
    12121 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
    12181 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    12241 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    12301 actgaattaa cgccgaatta attcctaggc caccatgttg ggcccggcgc gcc
//
