LOCUS       sequence-465508-        2709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IVT template for making modRNA encoding p53DD, a mutant p53 that 
            lacks DNA binding ability.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Blanch-Asensio A, Ploessl DS, Wang NB, Mummery CL, Galloway KE, 
            Davis RP
  TITLE     STRAIGHT-IN Dual: a platform for dual, single-copy integrations of 
            DNA payloads and gene circuits into human induced pluripotent stem 
            cells.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 
            10.1101/2024.10.17.616637.
  PUBMED    39464154
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 
            10.1101/2024.10.17.616637."
COMMENT     Sequence Label: pIVT-p53DD
FEATURES             Location/Qualifiers
     source          1..2709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    494..888
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(1175..1194)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1186..1995
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1253..1272)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2085..2673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2574..2593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatacgact cactataagg ctcctgggca acgtgctggt ctgtgtgctg gcccatcact
       61 ttggcaaagc acgctaccgg tcaatgactg ccatggagga gtcacagtcg gatatcagcc
      121 tcaagagagc gctgcccacc tgcacaagcg cctctccccc gcaaaagaaa aaaccacttg
      181 atggagagta tttcaccctc aagatccgcg ggcgtaaacg cttcgagatg ttccgggagc
      241 tgaatgaggc cttagagtta aaggatgccc atgctacaga ggagtctgga gacagcaggg
      301 ctcactccag ctacctgaag accaagaagg gccagtctac ttcccgccat aaaaaaacaa
      361 tggtcaagaa agtggggcct gactcagact aacaacctcg agctcaagct tcgaattcac
      421 cccaccagtg caggctgcct atcagaaagt ggtggctggt gtggctaatg ccctggccca
      481 caagtatcac taagctcgct ttcttgctgt ccaatttcta ttaaaggttc ctttgttccc
      541 taagtccaac tactaaactg ggggatatta tgaagggcct tgagcatctg gattctgcct
      601 aataaaaaac atttattttc attgcaatga tgtatttaaa ttatttctga atattttact
      661 aaaaagggaa tgtgggaggt cagtgcattt aaaacataaa gaaatgaaga gctagttcaa
      721 accttgggaa aatacactat atcttaaact ccatgaaaga aggtgaggct gcaaacagct
      781 aatgcacatt ggcaacagcc cctgatgcct atgccttatt catccctcag aaaaggattc
      841 aagtagaggc ttgatttgga ggttaaagtt ttgctatgct gtattttacc gaatcggatt
      901 ttgtttttaa tatttcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      961 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1021 aaaaaaaaaa aaaaaaaaac gcgtcgaagg gaattaattc ttgaagacga aagggccagg
     1081 tggcacgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1141 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1201 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1261 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1321 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1381 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1441 atccgtactc ctggtgatgc atggttactc accactgcga tccccggaaa aacagcattc
     1501 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     1561 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     1621 cgtctagctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     1681 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     1741 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     1801 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     1861 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     1921 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     1981 gatgagtttt tctaatcaga attggttaat tggttgtaac attattcaga ttgggccccg
     2041 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2101 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2161 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2221 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2281 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2341 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2401 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2461 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2521 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2581 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2641 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2701 ttcctggcc
//
