LOCUS       sequence-465543-        2854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His6-MBP-TEV-Rgs14 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Brown NE, Goswami D, Branch MR, Ramineni S, Ortlund EA, Griffin PR, 
            Hepler JR
  TITLE     Integration of G protein alpha (Galpha) signaling by the regulator 
            of G protein signaling 14 (RGS14).
  JOURNAL   J Biol Chem. 2015 Apr 3;290(14):9037-49. doi: 
            10.1074/jbc.M114.634329. Epub 2015 Feb 9.
  PUBMED    25666614
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.634329"; journalName: "J Biol Chem"; date: 
            "2015-04-3- 3"; volume: "290"; issue: "14"; pages: "9037-49"
COMMENT     Sequence Label: pLIC-His6-MBP-TEV-Rgs14
FEATURES             Location/Qualifiers
     source          1..2854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2697..2708
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcacttctgt gtgctgacac atccttggag ccttcagact gagccagact ccaggctcct
       61 cgctgggcac aaggaggctc ctgctagaca cttcctgtgg cagagaaaag aggtagtgag
      121 ctggtgtttc aggatgtaag ggcccggagg agcagagcga gctctctcca ctgcttgccc
      181 gccaccctga gagtgctggt cggtgtttcc acaatccttg tggtgggtgg ccccccgtgg
      241 aggggacctg tgagcaacgg aggcatgcca gggaagccca agcacttggg tgtccccaac
      301 gggcgcatgg tcctggctgt ctctgatgga gaactgacca gcacatcagg ttcccaggcc
      361 cagggtgagg gcagaggcag ctccctcagc atccacagcc tccccagtgg ccccagcagc
      421 cccttctcca ctgatgagca gcctgtggcc agctgggccc agtcctttga gcggctgctg
      481 caagacccac ggggtctggc ttacttcact gagttcctga agaaggaatt cagcgccgag
      541 aatgtaactt tctggcaagc ctgcgagcgt ttccaacaga tcccagccag cgacaccaaa
      601 cagctagctc aggaggccca caacatctac catgagttcc tgtccagcca ggcactgagc
      661 ccagtgaaca tcgaccgaca ggcctggctt agtgaggagg tgctggccca gcccaggccc
      721 gatatgttcc gagcacagca gcttcagatc ttcaatttga tgaagttcga cagctacgcg
      781 cgcttcgtca aatccccgct gtaccaagag tgcctgctgg ccgaggccga gggacgcccc
      841 ctgcgggaac ctggatcctc acacctcggg agcccggaca ctgcgaggaa gaagccaaag
      901 ctgaagcctg gaaagtcact gccgctcggt gtggaagagt tggggcagct gccgcttgct
      961 gagggccgcc ctctccgcaa gtcctttcgt agagagatgc cgggtggagc tgtgaattcg
     1021 gccctgcgac gagagtctca gggatccctg aattcttccg cgagtctgga cctgggcttc
     1081 cttgcctttg tgagcagcaa atccgagagc caccgaaaga gccttggaag tggagagggt
     1141 gagagcgaaa gccggcccgg gaagtactgc tgcgtgtatc tacctgatgg cacggcctcc
     1201 ttggccctgg ctcgacctgg cctcaccatc cgtgacatgc tggcaggcat ctgtgagaag
     1261 agaggcctct ctctacctga cattaaggtc tacctggtgg gcaaagaaca gaaggccctg
     1321 gtcctggatc aggactgcac cgtgctggca gaccaggaag tgcgactgga aaacaggatc
     1381 accttccagc tggagttggt cggcctggag cgtgtggtac ggatctctgc taagcccacc
     1441 aagcgtctgc aggaggcgct gcagcctatc ttggccaagc acggcctgag cctggatcag
     1501 gtggtcctac acaggccagg agagaagcag ctagtggatt tggagaatct agtgagctca
     1561 gtggcttcac agacactggt tttggacact cttccagatg caaagacaag ggaagccagc
     1621 agcatacctc cctgccgcag ccagggatgc ctccctagaa cccagaccaa ggacagtcac
     1681 cttcctccac tgtcctccag tttgtcggta gaagatgcca gtggttctac tgggaaacga
     1741 cagacctgcg acattgaagg cctagtggag ctgctgaatc gtgtgcagag cagcggggcc
     1801 catgaccaga ggggacttct tcgcaaagag gacctggtcc ttccagaatt tctgcagctt
     1861 ccttcccaaa gaccaggttc tcaggaggct ccaccataga ctgaatcaac tattcagccc
     1921 agggagggcc cttcagactc caccatccac tcggtcctct gacacctgtg taacaatcca
     1981 ggctgtctgc atggtgcctg ggcaaaccaa gcatgccacg ggtctgtgct gcatgccctg
     2041 tctgtaccag gagtgtcttt ggtccctttc tgctgtgggc aggcccacag aaagggccag
     2101 gaaggggtgg ctacttgttc tctcataggt ttacctacca attcctcaca gtaggccatc
     2161 aaggggaggt gggccttgtc ccttggcata agcatactga acatgaagag gtgactttgg
     2221 cagtgtcagc ctccttgact tgttctaggc tttagcaggg ggcaggaagg aggggctggc
     2281 ccctccttaa agagttggtg taagaaagac ctgggtgtac tactgggcaa cccctccctc
     2341 catccacaga ttttgctgta catatggatt ttatggttgg cttggggcag ctgggtttgt
     2401 cctggatgta tgatactgtt ataataataa ttattattat tcagccacga ggtgtctttt
     2461 ttttgtcttt ttttttcgga gctgggaacc gaacccaggg ccttgcgctt gttaggcaag
     2521 cgctctacca ctgagctaaa tccccaaccc catgaggtgt ctttcttgta tgccactctg
     2581 ccctgtgtag tctcactacc ccactgcctc tgtgccaggc caggaagttt tggggaacct
     2641 tagtgtatac ctcctcactt cttgttttca gaatctctct ccccaaggca taggatatag
     2701 aaggcagggg ggggggagca gaatggagag ggagagagcg ccacccccca ccccctactg
     2761 ttcttggcca tcacccagct gctccacaat gtccatggga gatgaatgta tcctttttgt
     2821 ttctgttctg tttctgttgg tgatacaatg tatc
//
