LOCUS       sequence-465544-       22097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 and Csy4 with 5' and 3' targets (II) for Lotus callus assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22097)
  AUTHORS   Bircheneder M, Schreiber T, Tissier A, Parniske M
  TITLE     A quantitative assay for the efficiency of RNA-guided genome editing
            in plants.
  JOURNAL   Plant J. 2024 Sep;119(5):2564-2577. doi: 10.1111/tpj.16931. Epub 
            2024 Jul 20.
  PUBMED    39032106
REFERENCE   2  (bases 1 to 22097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16931";
            journalName: "Plant J"; date: "2024-09"; volume: "119"; issue: "5"; 
            pages: "2564-2577"
COMMENT     Sequence Label: pMB109
FEATURES             Location/Qualifiers
     source          1..22097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..158
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     73..92
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(173..190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(344..932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(424..443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1002..1021)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1003..1021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1026..1042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1026..1042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1144..1163)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1155..1964
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1222..1241)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    2220..2244
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2371..2550
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2403..2586
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2607..3632
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             5062..5082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5107..5116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5113..9216
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5113..9216
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9241..9261
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_RNA        11633..11708
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        12929..13004
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             14529..14546
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    14557..14576
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     CDS             join(14616..14618,14619..14621,14622..15332)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(14661..14682)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(14922..14941)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     15269..15290
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             15345..15365
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      15394..15646
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      17743..17991
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    18055..18079
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             19380..20009
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             20438..21511
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      21577..21771
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(22007..22029)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
       61 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
      121 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
      181 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      301 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      781 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      961 ctgacgctca taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     1021 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     1081 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     1141 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     1201 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     1261 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     1321 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     1381 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     1441 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     1501 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     1561 attgtccttt taacagcgat cgcgtatttc gtcttgctca ggcgcaatca cgaatgaata
     1621 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     1681 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     1741 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     1801 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     1861 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     1921 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     1981 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     2041 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agacccctgc cggtaacatg
     2101 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
     2161 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     2221 ggcaggatat attgtggtgt aaacaaattg acgcttagac ggattacggg tctcagcggt
     2281 acggcgggat catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg
     2341 ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc
     2401 gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca
     2461 aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac
     2521 gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
     2581 tctgcaccgg atcttctgga agcaccatga aaaagcctga actcaccgcg acgtctgtcg
     2641 agaagtttct gatcgaaaag ttcgacagcg tgtccgacct gatgcagctc tcggagggcg
     2701 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     2761 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     2821 tcccgattcc ggaagtgctt gacattgggg agtttagcga gagcctgacc tattgcatct
     2881 cccgccgttc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     2941 tacaaccggt cgcggaggct atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     3001 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     3061 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     3121 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     3181 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     3241 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     3301 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     3361 ggcatccgga gcttgcagga tcgccacgac tccgggcgta tatgctccgc attggtcttg
     3421 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     3481 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     3541 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     3601 gccccagcac tcgtccgagg gcaaagaaat agaagggcta agacctaagc tagcctagaa
     3661 tccggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct
     3721 atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg
     3781 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     3841 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgaccttg agtgacctaa
     3901 gctagcctag tgtcgggcgc ggggagagag gattttgagg aaataattaa ttgaattact
     3961 tgtattattg ataaagtaat ttagataagt tgttagtaca acttattgca actatggata
     4021 gacaaaaatc acttatttta aggggagaag tatataacaa cttattataa aatttctgag
     4081 cagccggcct cctccaatca atcatagaga gtggagccca ttctggaaaa ccaaggaacc
     4141 cccaacttgc actggtgcgg tggcccaatt caaaaaaaac ggggcccagc aaagtaacgc
     4201 cgttgtaacg atttatcaat ccaaactcaa aaggcggcga gatgcgttta actccgtgaa
     4261 attaacaaac cgccaacaac ttgcaatttg caactaccgt ttccaagaag aactcaacca
     4321 cacaacgtat cctatcccaa accacacgca actagtgacg cgtcataagg acacgtgtca
     4381 caatttgact ggttaataat ttcaccgctt ttgctataaa ttacctccaa tccccttagc
     4441 ttcttcacaa ttcagttccc aaccctaaca attcttgttc atatcgcttc tctctacttt
     4501 caaggtatga tccaatttct ctcttctttc tctgtaatcc tttcgttgag ttttgtttcc
     4561 gatcaatcat aggtagtttt cttgtttcga agcatgagat ctaggaattt tttgtgattt
     4621 tccaaaattg agatcggttt gaaattgaat tttacagctt gaatctcaga tcttgtttta
     4681 tcaatgtttt cgatggcttg cgatgtagat ctatgataat tgtggttcag ttttgttagg
     4741 aatcgatttc ggtttagcaa ttgcagatta attagggttt ccaattgaat tcttcagatc
     4801 cgttatggaa ttatgtcaaa taatttattc aaattggaaa ttattgttag atccactctt
     4861 aatctgtttg atccaagctt caattagggt tttcacttgt ttcaatttct tgttatggat
     4921 tctgatttat ctgttgatgt tagatccact cttaatctgt ttgatccaag cgttaattag
     4981 ggttttcact tgtttcaatt tcgtgtgttg gattctgatt tatctgttgt tgatgtgatt
     5041 acagtctgaa caatgctgca gcctaagaag aagagaaagg ttggaggagt cgactcgagt
     5101 gcggccgcca ccatggataa gaagtactct atcggactcg atatcggaac taactctgtg
     5161 ggatgggctg tgatcaccga tgagtacaag gtgccatcta agaagttcaa ggttctcgga
     5221 aacaccgata ggcactctat caagaaaaac cttatcggtg ctctcctctt cgattctggt
     5281 gaaactgctg aggctaccag actcaagaga accgctagaa gaaggtacac cagaagaaag
     5341 aacaggatct gctacctcca agagatcttc tctaacgaga tggctaaagt ggatgattca
     5401 ttcttccaca ggctcgaaga gtcattcctc gtggaagaag ataagaagca cgagaggcac
     5461 cctatcttcg gaaacatcgt tgatgaggtg gcataccacg agaagtaccc tactatctac
     5521 cacctcagaa agaagctcgt tgattctact gataaggctg atctcaggct catctacctc
     5581 gctctcgctc acatgatcaa gttcagagga cacttcctca tcgagggtga tctcaaccct
     5641 gataactctg atgtggataa gttgttcatc cagctcgtgc agacctacaa ccagcttttc
     5701 gaagagaacc ctatcaacgc ttcaggtgtg gatgctaagg ctatcctctc tgctaggctc
     5761 tctaagtcaa gaaggcttga gaacctcatt gctcagctcc ctggtgagaa gaagaacgga
     5821 cttttcggaa acttgatcgc tctctctctc ggactcaccc ctaacttcaa gtctaacttc
     5881 gatctcgctg aggatgcaaa gctccagctc tcaaaggata cctacgatga tgatctcgat
     5941 aacctcctcg ctcagatcgg agatcagtac gctgatttgt tcctcgctgc taagaacctc
     6001 tctgatgcta tcctcctcag tgatatcctc agagtgaaca ccgagatcac caaggctcca
     6061 ctctcagctt ctatgatcaa gagatacgat gagcaccacc aggatctcac acttctcaag
     6121 gctcttgtta gacagcagct cccagagaag tacaaagaga ttttcttcga tcagtctaag
     6181 aacggatacg ctggttacat cgatggtggt gcatctcaag aagagttcta caagttcatc
     6241 aagcctatcc tcgagaagat ggatggaacc gaggaactcc tcgtgaagct caatagagag
     6301 gatcttctca gaaagcagag gaccttcgat aacggatcta tccctcatca gatccacctc
     6361 ggagagttgc acgctatcct tagaaggcaa gaggatttct acccattcct caaggataac
     6421 agggaaaaga ttgagaagat tctcaccttc agaatccctt actacgtggg acctctcgct
     6481 agaggaaact caagattcgc ttggatgacc agaaagtctg aggaaaccat caccccttgg
     6541 aacttcgaag aggtggtgga taagggtgct agtgctcagt ctttcatcga gaggatgacc
     6601 aacttcgata agaaccttcc aaacgagaag gtgctcccta agcactcttt gctctacgag
     6661 tacttcaccg tgtacaacga gttgaccaag gttaagtacg tgaccgaggg aatgaggaag
     6721 cctgcttttt tgtcaggtga gcaaaagaag gctatcgttg atctcttgtt caagaccaac
     6781 agaaaggtga ccgtgaagca gctcaaagag gattacttca agaaaatcga gtgcttcgat
     6841 tcagttgaga tttctggtgt tgaggatagg ttcaacgcat ctctcggaac ctaccacgat
     6901 ctcctcaaga tcattaagga taaggatttc ttggataacg aggaaaacga ggatatcttg
     6961 gaggatatcg ttcttaccct caccctcttt gaagatagag agatgattga agaaaggctc
     7021 aagacctacg ctcatctctt cgatgataag gtgatgaagc agttgaagag aagaagatac
     7081 actggttggg gaaggctctc aagaaagctc attaacggaa tcagggataa gcagtctgga
     7141 aagacaatcc ttgatttcct caagtctgat ggattcgcta acagaaactt catgcagctc
     7201 atccacgatg attctctcac ctttaaagag gatatccaga aggctcaggt ttcaggacag
     7261 ggtgatagtc tccatgagca tatcgctaac ctcgctggat ctcctgcaat caagaaggga
     7321 atcctccaga ctgtgaaggt tgtggatgag ttggtgaagg tgatgggaag gcataagcct
     7381 gagaacatcg tgatcgaaat ggctagagag aaccagacca ctcagaaggg acagaagaac
     7441 tctagggaaa ggatgaagag gatcgaggaa ggtatcaaag agcttggatc tcagatcctc
     7501 aaagagcacc ctgttgagaa cactcagctc cagaatgaga agctctacct ctactacctc
     7561 cagaacggaa gggatatgta tgtggatcaa gagttggata tcaacaggct ctctgattac
     7621 gatgttgatc atatcgtgcc acagtcattc ttgaaggatg attctatcga taacaaggtg
     7681 ctcaccaggt ctgataagaa caggggtaag agtgataacg tgccaagtga agaggttgtg
     7741 aagaaaatga agaactattg gaggcagctc ctcaacgcta agctcatcac tcagagaaag
     7801 ttcgataact tgactaaggc tgagagggga ggactctctg aattggataa ggcaggattc
     7861 atcaagaggc agcttgtgga aaccaggcag atcactaagc acgttgcaca gatcctcgat
     7921 tctaggatga acaccaagta cgatgagaac gataagttga tcagggaagt gaaggttatc
     7981 accctcaagt caaagctcgt gtctgatttc agaaaggatt tccaattcta caaggtgagg
     8041 gaaatcaaca actaccacca cgctcacgat gcttacctta acgctgttgt tggaaccgct
     8101 ctcatcaaga agtatcctaa gctcgagtca gagttcgtgt acggtgatta caaggtgtac
     8161 gatgtgagga agatgatcgc taagtctgag caagagatcg gaaaggctac cgctaagtat
     8221 ttcttctact ctaacatcat gaatttcttc aagaccgaga ttaccctcgc taacggtgag
     8281 atcagaaaga ggccactcat cgagacaaac ggtgaaacag gtgagatcgt gtgggataag
     8341 ggaagggatt tcgctaccgt tagaaaggtg ctctctatgc cacaggtgaa catcgttaag
     8401 aaaaccgagg tgcagaccgg tggattctct aaagagtcta tcctccctaa gaggaactct
     8461 gataagctca ttgctaggaa gaaggattgg gaccctaaga aatacggtgg tttcgattct
     8521 cctaccgtgg cttactctgt tctcgttgtg gctaaggttg agaagggaaa gagtaagaag
     8581 ctcaagtctg ttaaggaact tctcggaatc actatcatgg aaaggtcatc tttcgagaag
     8641 aacccaatcg atttcctcga ggctaaggga tacaaagagg ttaagaagga tctcatcatc
     8701 aagctcccaa agtactcact cttcgaactc gagaacggta gaaagaggat gctcgcttct
     8761 gctggtgagc ttcaaaaggg aaacgagctt gctctcccat ctaagtacgt taactttctt
     8821 tacctcgctt ctcactacga gaagttgaag ggatctccag aagataacga gcagaagcaa
     8881 cttttcgttg agcagcacaa gcactacttg gatgagatca tcgagcagat ctctgagttc
     8941 tctaaaaggg tgatcctcgc tgatgcaaac ctcgataagg tgttgtctgc ttacaacaag
     9001 cacagagata agcctatcag ggaacaggca gagaacatca tccatctctt cacccttacc
     9061 aacctcggtg ctcctgctgc tttcaagtac ttcgatacaa ccatcgatag gaagagatac
     9121 acctctacca aagaagtgct cgatgctacc ctcatccatc agtctatcac tggactctac
     9181 gagactagga tcgatctctc acagctcggt ggtgattcaa gggctgataa gggactgcag
     9241 cctaagaaga agagaaaggt tggaggagtc gactcgagtg cgtaaaatca tacagcattc
     9301 ccagaaagag aaacagaaga aatatacaaa ctttcatttt gagagcagca cctcgtctat
     9361 tgattgcaga taatatgctt ctcatttgta tttccttttg attatttttg tttctatccc
     9421 tttgtttgag tcaatctcaa atattcggtc attgttggta tgaaaaatca agcagttcat
     9481 gttaagagtc aatttaaaat taatattttt atatagagtt gtatgtgaaa tgatgttgtg
     9541 atttggtata tatggataaa gagcttgtca gttcattttg ctctcatttt tttggtatcc
     9601 aaataagaaa cacaaaaggg atatgtccct ctactatcaa atattagtta taagtattca
     9661 tgttatacta ttcgatattt tctaccccaa tcgttaccta tttaaaagta tttacccctc
     9721 catctatcaa acccctggac ccagctttcc tattacatgt ggcttcatct taagccccca
     9781 aacctttttc ttatttttga tttttaaagg ctcatcttaa aatttattac tcaaattaat
     9841 acctcttaat aacccacctc aaggacccag taattaaata tccaattagc tccagtaatt
     9901 ggggttcata ttagctccag tcttaaattt taaaggcgat gatcgtattc ctccacttgg
     9961 ttcatttata ctcaaagaat actcaatgtc tttagtgttt agataacttt ttgtaaatca
    10021 tatagattgt tttaacaaaa aacaattcaa tagtagattt tcacatgaaa gttacataaa
    10081 aattctttaa aattactttc tcaaaaaatt gttccaaaca tattatccca caattaaact
    10141 caatctgttt ttcgaaacct aaatcaaaac caatccaact accttatata atatataatc
    10201 aatacattgt aaagaactgc atgttctttt aaattttggg ggcaaagtta ttccgtacgt
    10261 tcacacatgt actaatagga ggtaataaat gatatgtgaa acaatcgagg tgtaaacaag
    10321 ctagcattga gcaggcctcc cagctttcgt ccgtatcatc ggtttcgaca acgttcgtca
    10381 agttcaatgc atcagtttca ttgcccacac accagaatcc tactaagttt gagtattatg
    10441 gcattggaaa agctgttttc ttctatcatt tgttctgctt gtaatttact gtgttctttc
    10501 agtttttgtt ttcggacatc aaaatgcaaa tggatggata agagttaata aatgatatgg
    10561 tccttttgtt cattctcaaa ttattattat ctgttgtttt tactttaatg ggttgaattt
    10621 aagtaagaaa ggaactaaca gtgtgatatt aaggtgcaat gttagacata taaaacagtc
    10681 tttcacctct ctttggttat gtcttgaatt ggtttgtttc ttcacttatc tgtgtaatca
    10741 agtttactat gagtctatga tcaagtaatt atgcaatcaa gttaagtaca gtataggctt
    10801 tgtcattggc ggaatctttg agagggaata aaagaaatac ttccaaaaat ccattagaag
    10861 aactataaat cttctgggca tgtaaatata acgtaaaata tatgttatgt tacaacctta
    10921 tttcattcac attttaattt tttttcacac aatttttatt taattttgtt tttatctcta
    10981 ttttattttc atatgataat acatattatc tctttatttt actttatgtg tctctttgct
    11041 ctcacatttt atttcttaac taacatattc tcattagcag tagctatcgt aagactaatc
    11101 tcacacaata attaattatc cattctattt atgaattttt atcatttaat aattctttac
    11161 ctgtgcaaat tttgaaggaa aaagtttctg aatataaaat aacaataata ctaattagat
    11221 gtcaaatcaa atcacccagg ctagacaata gtggtaatga agaaggctag aggtagctct
    11281 gtgcctctag gggagtgaga gacgtttttc atttattggt gtgttgaaac ttgaaacgtt
    11341 gatgttctcc acagctccac gtattctttc gtcctagaac gaactagagc aacataatct
    11401 gtctcttcct gaagcttcat tgtttttctt tccttctagc agcatcttct taacagacga
    11461 agaagggact cagatgcttg cttgcatgct tccaaagttt ggagctgagg agacttgtgc
    11521 tatttgagtc tgttttagca ttcgcaagcc ccacaccgga taaacataca gaaatctgag
    11581 tgtttataac caaatagtca gcaacaaggt tcgccgttaa tttgagagtc cagttttaga
    11641 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
    11701 gtcggtgctt tttttggcaa aaattttcag attttttctt catctgtaga tttctgggtt
    11761 tttttttccg tttcgtgaat cataagtgaa gttttggatg caaatctgcg cgaaaaaagt
    11821 tggacctgca atgagcttat ttagatagct aagacaaagt gattggtccg ttgtttcagt
    11881 tctgattgtc agagagtttg tttcgagtcg gcgacaccaa tgcgttttgt taaccagatt
    11941 tcgggtaaga aatgtatcga gagtttgttt ccagacggct acatcatttt cttatgaagg
    12001 gtgaaattag atagaccaaa gattgaaaca caacatttct ttcacaaaaa tataataaac
    12061 ttgatagcat ttaggatcag ccacctgacc taagctagcc tagaaggaat ctttgagagg
    12121 gaataaaaga aatacttcca aaaatccatt agaagaacta taaatcttct gggcatgtaa
    12181 atataacgta aaatatatgt tatgttacaa ccttatttca ttcacatttt aatttttttt
    12241 cacacaattt ttatttaatt ttgtttttat ctctatttta ttttcatatg ataatacata
    12301 ttatctcttt attttacttt atgtgtctct ttgctctcac attttatttc ttaactaaca
    12361 tattctcatt agcagtagct atcgtaagac taatctcaca caataattaa ttatccattc
    12421 tatttatgaa tttttatcat ttaataattc tttacctgtg caaattttga aggaaaaagt
    12481 ttctgaatat aaaataacaa taatactaat tagatgtcaa atcaaatcac ccaggctaga
    12541 caatagtggt aatgaagaag gctagaggta gctctgtgcc tctaggggag tgagagacgt
    12601 ttttcattta ttggtgtgtt gaaacttgaa acgttgatgt tctccacagc tccacgtatt
    12661 ctttcgtcct agaacgaact agagcaacat aatctgtctc ttcctgaagc ttcattgttt
    12721 ttctttcctt ctagcagcat cttcttaaca gacgaagaag ggactcagat gcttgcttgc
    12781 atgcttccaa agtttggagc tgaggagact tgtgctattt gagtctgttt tagcattcgc
    12841 aagccccaca ccggataaac atacagaaat ctgagtgttt ataaccaaat agtcagcaac
    12901 aaggttcgac gtatcttcgg ccatgaaggt tttagagcta gaaatagcaa gttaaaataa
    12961 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tggcaaaaat
    13021 tttcagattt tttcttcatc tgtagatttc tgggtttttt tttccgtttc gtgaatcata
    13081 agtgaagttt tggatgcaaa tctgcgcgaa aaaagttgga cctgcaatga gcttatttag
    13141 atagctaaga caaagtgatt ggtccgttgt ttcagttctg attgtcagag agtttgtttc
    13201 gagtcggcga caccaatgcg ttttgttaac cagatttcgg gtaagaaatg tatcgagagt
    13261 ttgtttccag acggctacat cattttctta tgaagggtga aattagatag accaaagatt
    13321 gaaacacaac atttctttca caaaaatata ataaacttga tagcatttag gatcagctgt
    13381 ctgtcgcggg gagagaggat tttgaggaaa taattaattg aattacttgt attattgata
    13441 aagtaattta gataagttgt tagtacaact tattgcaact atggatagac aaaaatcact
    13501 tattttaagg ggagaagtat ataacaactt attataaaat ttctgagcag ccggcctcct
    13561 ccaatcaatc atagagagtg gagcccattc tggaaaacca aggaaccccc aacttgcact
    13621 ggtgcggtgg cccaattcaa aaaaaacggg gcccagcaaa gtaacgccgt tgtaacgatt
    13681 tatcaatcca aactcaaaag gcggcgagat gcgtttaact ccgtgaaatt aacaaaccgc
    13741 caacaacttg caatttgcaa ctaccgtttc caagaagaac tcaaccacac aacgtatcct
    13801 atcccaaacc acacgcaact agtgacgcgt cataaggaca cgtgtcacaa tttgactggt
    13861 taataatttc accgcttttg ctataaatta cctccaatcc ccttagcttc ttcacaattc
    13921 agttcccaac cctaacaatt cttgttcata tcgcttctct ctactttcaa ggtatgatcc
    13981 aatttctctc ttctttctct gtaatccttt cgttgagttt tgtttccgat caatcatagg
    14041 tagttttctt gtttcgaagc atgagatcta ggaatttttt gtgattttcc aaaattgaga
    14101 tcggtttgaa attgaatttt acagcttgaa tctcagatct tgttttatca atgttttcga
    14161 tggcttgcga tgtagatcta tgataattgt ggttcagttt tgttaggaat cgatttcggt
    14221 ttagcaattg cagattaatt agggtttcca attgaattct tcagatccgt tatggaatta
    14281 tgtcaaataa tttattcaaa ttggaaatta ttgttagatc cactcttaat ctgtttgatc
    14341 caagcttcaa ttagggtttt cacttgtttc aatttcttgt tatggattct gatttatctg
    14401 ttgatgttag atccactctt aatctgtttg atccaagcgt taattagggt tttcacttgt
    14461 ttcaatttcg tgtgttggat tctgatttat ctgttgttga tgtgattaca gtctgaacaa
    14521 tgggcagcca ccaccaccac caccacagcg gaatgagttc actgccgtat aggcagctaa
    14581 gaaaatcagg tggcaccggc ggcagcggcg gatccatggt gagcaagggc gaggagctgt
    14641 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
    14701 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
    14761 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc
    14821 tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
    14881 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
    14941 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
    15001 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
    15061 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
    15121 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
    15181 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga
    15241 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
    15301 ggatcactct cggcatggac gagctgtaca agaagggact gcagcctaag aagaagagaa
    15361 aggttggagg agtcgactcg agtgcgtaaa atcgatcgtt caaacatttg gcaataaagt
    15421 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
    15481 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
    15541 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
    15601 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatctgag tgacctaagc
    15661 tagcctagtg tctgaggcgg ccaacaaact attcgtcacg tcgacaaaat ttagaacgaa
    15721 cttaattatg atctcaaata cattgataca tatctcatct agatctaggt tatcattatg
    15781 taagaaagtt ttgacgaata tggcacgaca aaatggctag actcgatgta attggtatct
    15841 caactcaaca ttatacttat accaaacatt agttagacaa aatttaaaca actatttttt
    15901 atgtatgcaa gagtcagcat atgtataatt gattcagaat cgttttgacg agttcggatg
    15961 tagtagtagc cattatttaa tgtacatact aatcgtgaat agtgaatatg atgaaacatt
    16021 gtatcttatt gtataaatat ccataaacac atcatgaaag acactttctt tcacggtctg
    16081 aattaattat gatacaattc taatagaaaa cgaattaaat tacgttgaat tgtatgaaat
    16141 ctaattgaac aagccaacca cgacgacgac taacgttgcc tggattgact cggtttaagt
    16201 taaccactaa aaaaacggag ctgtcatgta acacgcggat cgagcaggtc acagtcatga
    16261 agccatcaaa gcaaaagaac taatccaagg gctgagatga ttaattagtt taaaaattag
    16321 ttaacacgag ggaaaaggct gtctgacagc caggtcacgt tatctttacc tgtggtcgaa
    16381 atgattcgtg tctgtcgatt ttaattattt ttttgaaagg ccgaaaataa agttgtaaga
    16441 gataaacccg cctatataaa ttcatatatt ttcctctccg ctttgaattg tctcgttgtc
    16501 ctcctcactt tcatcagccg ttttgaatct ccggcgactt gacagagaag aacaaggaag
    16561 aagtctaaga gagaaagtaa gagataatcc aggagattca ttctccgttt tgaatcttcc
    16621 tcaatctcat cttcttccgc tctttctttc caaggtaata ggaactttct ggatctactt
    16681 tatttgctgg atctcgatct tgttttctca atttccttga gatctggaat tcgtttaatt
    16741 tggatctgtg aacctccact aaatcttttg gttttactag aatcgatcta agttgaccga
    16801 tcagttagct cgattatagc taccagaatt tggcttgacc ttgatggaga gatccatgtt
    16861 catgttacct gggaaatgat ttgtatatgt gaattgaaat ctgaactgtt gaagttagat
    16921 tgaatctgaa cactgtcaat gttagattga atctgaacac tgtttaaggt tagatgaagt
    16981 ttgtgtatag attcttcgaa actttaggat ttgtagtgtc gtacgttgaa cagaaagcta
    17041 tttctgattc aatcagggtt tatttgactg tattgaactc tttttgtgtg tttgcagctc
    17101 ataaaaatct gccttggact ctcaaattaa cggcgaaaca ccatggatca ttatcttgat
    17161 attagactta gacctgatcc tgaatttcct cctgctcaac ttatgtctgt tctttttgga
    17221 aagcttcatc aagctcttgt tgctcaagga ggagatagaa ttggagtttc ttttcctgat
    17281 cttgatgaat ctagatctag acttggagaa agacttagaa ttcatgcttc tgctgatgat
    17341 cttagagctc ttcttgctag accttggctt gaaggactta gagatcatct tcaatttgga
    17401 gaacctgctg ttgttcctca tcctactcct tatagacaag tttctagagt tcaagctaag
    17461 tctaatcctg aaagacttag aagaagattg atgaggagac atgatctttc tgaagaagaa
    17521 gctagaaaga gaattcctga tactgttgct agagctcttg atcttccttt tgttactctt
    17581 agatctcaat ctactggaca acattttaga ctttttatta gacatggacc tcttcaagtt
    17641 actgctgaag aaggaggatt tacttgttat ggactttcta agggaggatt tgttccttgg
    17701 ttttgaaagg tttcacgtat cttcggccat gaagaggaat catatgaaga tgaagatgaa
    17761 atatttggtg tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt tcttggcttg
    17821 ttgtgttatg aatttgtggc tttttctaat attaaatgaa tgtaagatct cattataatg
    17881 aataaacaaa tgtttctata atccattgtg aatgttttgt tggatctctt ctgcagcata
    17941 taactactgt atgtgctatg gtatggacta tggaatatga ttaaagataa gtgagtgacc
    18001 taagctagcc tagtgtctct gtctgtgaga cctctggttt aaactatcag tgtttgacag
    18061 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
    18121 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
    18181 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
    18241 acgttcagtg cagccttctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
    18301 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
    18361 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
    18421 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
    18481 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
    18541 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
    18601 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
    18661 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
    18721 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
    18781 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
    18841 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
    18901 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
    18961 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
    19021 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
    19081 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
    19141 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcaca tctcaaccgt gcggctgcat
    19201 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
    19261 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
    19321 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
    19381 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
    19441 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
    19501 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
    19561 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
    19621 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
    19681 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
    19741 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
    19801 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
    19861 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
    19921 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
    19981 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
    20041 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
    20101 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
    20161 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
    20221 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
    20281 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
    20341 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct
    20401 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
    20461 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
    20521 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
    20581 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
    20641 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
    20701 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
    20761 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
    20821 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
    20881 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
    20941 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
    21001 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
    21061 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
    21121 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
    21181 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
    21241 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
    21301 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
    21361 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
    21421 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
    21481 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
    21541 cctagcaggg gaaaaaggtc gaaaaggact ctttcctgtg gatagcacgt acattgggaa
    21601 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
    21661 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
    21721 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
    21781 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
    21841 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
    21901 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
    21961 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    22021 agctcccgga gacagtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    22081 agggcgcgtc agcgggt
//
