LOCUS       sequence-465546-        8864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mNeonGreen-tagged PIP3 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Nikolatou K, Sandilands E, Roman-Fernandez A, Cumming EM, Freckmann 
            E, Lilla S, Buetow L, McGarry L, Neilson M, Shaw R, Strachan D, 
            Miller C, Huang DT, McNeish IA, Norman JC, Zanivan S, Bryant DM
  TITLE     PTEN deficiency exposes a requirement for an ARF GTPase module for 
            integrin-dependent invasion in ovarian cancer.
  JOURNAL   EMBO J. 2023 Sep 18;42(18):e113987. doi: 10.15252/embj.2023113987. 
            Epub 2023 Aug 14.
  PUBMED    37577760
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-09-18"; volume: "42(18):e113987. doi"; pages: " 
            10.15252/embj.2023113987. Epub 2023 Aug 14"
COMMENT     Sequence Label: mNG-PH-CYTH3-2G
FEATURES             Location/Qualifiers
     source          1..8864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    418..543
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             590..770
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(771..997)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1025..1045)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1025..1043)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1064..1080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1064..1080)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1077..1099)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1088..1104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1112..1118,1119..1136,1137..1142))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1157..1178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1295..1312)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1466..2054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1546..1565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2225..3016,3017..3085))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2848..2867
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3086..3190)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3216..3671)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3353..3374)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3565..3584
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3798..3820
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3812..3829
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3813..3829
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3839..3858
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3839..3857
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3878..4013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4053..4174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4064..4083)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4118..4137
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4252..4485
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4557..5145
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4663..4674
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5072..5092
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5692..6399)
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      6135..6144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6396..6405
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6444..6647)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6473..6493)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6648..6951
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7068..7185
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7245..7640)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(7652..8152)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7715..7733)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(8327..8371)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8556..8789
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tttactactt tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca
       61 ggtctgaaga tcagcggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc
      121 tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag
      181 ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg
      241 tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc
      301 gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc
      361 ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac
      421 gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac
      481 cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga
      541 gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt
      601 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
      661 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
      721 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc gtttatgtat
      781 cgagctaggc acttaaatac aatatctctg caatgcggca attcagtggt tcgtccaatc
      841 catgtcagac ccgtctgttg ccttcctaat aaggcacgat cgtaccacct tacttccacc
      901 aatcggcatg cacggtgctt tttctctcct tgtaaggcat gttgctaact catcgttacc
      961 atgttgcaag actacaagag tattgcataa gactacatta agcttgcagc tccagctttt
     1021 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     1081 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1141 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1201 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1261 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1321 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1381 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1441 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1501 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1561 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1621 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1681 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1741 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1801 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1861 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1921 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1981 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2041 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2101 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2161 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2221 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2281 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2341 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2401 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2461 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2521 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2581 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2641 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2701 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2761 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2821 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2881 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2941 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3001 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3061 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3121 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3181 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     3241 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     3301 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     3361 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     3421 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     3481 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     3541 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     3601 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     3661 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     3721 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     3781 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     3841 atacgactca ctatagggcg aattgggtac gtccctaggc ctccaaaaaa gcctcctcac
     3901 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     3961 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     4021 gttaggggcg ggatagctag agccagacat gataagatac attgatgagt ttggacaaac
     4081 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     4141 atttgtaacc attataagct gcaataaaca agttcctctc actctctgat attcatttct
     4201 ttgcaagtta taaatactga ataataagat gacatgaact actatacgta ctgctagaga
     4261 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     4321 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     4381 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacaag
     4441 caaaaagcag atcttgtctt cgttgggagt gaattagccc ttccagtccc cccttttctt
     4501 ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa gtcgacgcgg
     4561 ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg
     4621 ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac
     4681 gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa
     4741 ggacgatgat ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt
     4801 ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc
     4861 gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc
     4921 agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag
     4981 agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc
     5041 atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag
     5101 aataccagtc aatctttcac aaattttgta atccagaggt tgattgtcga cttaacgcgc
     5161 caccggtgat cacgtacggg atcccgggcc cgcggtaccg tcgactgcag aattctattt
     5221 cttattggca atcctccttt tcctcgtggc caacatgtca tagaacgggt ccctactgat
     5281 gcttgctttg atggacttca tccactcctc cttttcctcc gggctggggg cagagatccg
     5341 gtacacaacg tggttgccct ccaccacacg gccatcggcc tccgtcttgc aggccttgat
     5401 gacttgacct ttgtgactgg ggttatacag ctcaaagcag ttcggcttcc gggggtcctc
     5461 cacctccctg atgctgaggt tctccagggg gatgatgccc ctgggctcct tgtccgtggt
     5521 gtactcaaag tagtagaggc agttatctgt gaggatgaac cagcgccgtt tccaggtctt
     5581 cacacgaccc cccagcttca gcagccagcc ttctcggtct gggttgaaga acgtgtgtgt
     5641 caggtcattt ccgtcgtctt ctgggatctt aaacggggat ctgagtccgg acttgtacag
     5701 ctcgtccatg cccatcacat cggtaaaggc cttttgccac tccttgaagt tgagctcggt
     5761 cttggagtgc ttgagctccg tcttacggaa cacgtacatc ggctggttct tcagatagtt
     5821 agccgccatt ggcttggcaa aggtgtaggt ggtccgcgca gtgctccggt agcgcttgcc
     5881 atttccagtg gtgtaactcc acttaaaggt actgatgatg gttttgtcgt tggggtaagt
     5941 cttcttcgac ctgcaccagt ccgcagcggt cagcgagttg gtcatcacag gaccgtcagc
     6001 agggaaacca gtccccttca cctgggcctc tcctttgatg tggcttccct cgtaggtgta
     6061 gcggtagtta acagtaaggg aggcaccatc ttcaaactgc attgtgcgat ggacttggta
     6121 gccggagcca tctaccatgg cggcctggaa aggcgacatc ccgtcagggt agggcaggta
     6181 ctgatggaag ccatacccga tatgagggac cagaatccag ggggagaact ggaggtcacc
     6241 cttggtggac ttcaggttta actcctcata accatcattt ggattgccgg tgccctgacc
     6301 caccatgtca aagtccacac cgttgatgga gccaaagatg tgtaactcat gtgtcgctgg
     6361 gagagaggcc atgttatcct cctcgccctt gctcaccatg gtggcgaccg gtagcgctag
     6421 cggatctgac ggttcactaa accagctctg cttatataga cctcccaccg tacacgccta
     6481 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
     6541 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag
     6601 tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg
     6661 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
     6721 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
     6781 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
     6841 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
     6901 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
     6961 atatatgggc tatgaactaa tgaccccgta attgattact attaataacc ccgggccccc
     7021 tcgagccggc gccaaagtgg atctctgctg tccctgtaat aaacccgaaa attttgaatt
     7081 tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt
     7141 ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg gatgaatact
     7201 gccatttgtc tcaagatcta gaattcttac tactgcagct gcaggccctc ccacacataa
     7261 ccagagggca gcaattcacg aatcccaact gccgtcggct gtccatcact gtccttcact
     7321 atggctttga tcccaggatg cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc
     7381 aagatgcccc tgttctcatt tccgatcgcg acgatacaag tcaggttgcc agctgccgca
     7441 gcagcagcag tgcccagcac cacgagttct gcacaaggtc ccccagtaaa atgatataca
     7501 ttgacaccag tgaagatgcg gccgtcgcta gagagagctg cgctggcgac gctgtagtct
     7561 tcagagatgg ggatgctgtt gattgtagcc gttgctcttt caatgagggt ggattcttct
     7621 tgagacaaag gcttggccat ggtggtaccc cctggggaga gaggtcggtg attcggtcaa
     7681 cgagggagcc gactgccgac gtgcgctccg gaggcttgca gaatgcggaa caccgcgcgg
     7741 gcaggaacag ggcccacact accgccccac accccgcctc ccgcaccgcc ccttcccggc
     7801 cgctgctctc ggcgcgccct gctgagcagc cgctattggc cacagcccat cgcggtcggc
     7861 gcgctgccat tgctccctgg cgctgtccgt ctgcgagggt actagtgaga cgtgcggctt
     7921 ccgtttgtca cgtccggcac gccgcgaacc gcaaggaacc ttcccgactt aggggcggag
     7981 caggaagcgt cgccgggggg cccacaaggg tagcggcgaa gatccgggtg acgctgcgaa
     8041 cggacgtgaa gaatgtgcga gacccagggt cggcgccgct gcgtttcccg gaaccacgcc
     8101 cagagcagcc gcgtccctgc gcaaacccag ggctgccttg gaaaaggcgc aagggtcccc
     8161 tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca
     8221 ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga
     8281 ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt
     8341 tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta
     8401 aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt
     8461 gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg
     8521 tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc
     8581 tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca
     8641 gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca
     8701 ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg
     8761 ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca
     8821 ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatt
//
