LOCUS       sequence-465605-        9712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of catalytically-inactive PspCas13b fused with 4EHP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Apostolopoulos A, Kawamoto N, Chow SYA, Tsuiji H, Ikeuchi Y, 
            Shichino Y, Iwasaki S
  TITLE     dCas13-mediated translational repression for accurate gene silencing
            in mammalian cells.
  JOURNAL   Nat Commun. 2024 Mar 11;15(1):2205. doi: 10.1038/s41467-024-46412-7.
  PUBMED    38467613
REFERENCE   2  (bases 1 to 9712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-03-11"; volume: "15(1):2205. doi"; pages: " 
            10.1038/s41467-024-46412-7"
COMMENT     Sequence Label: pCAGEN-dPspCas13b-4EHP-NES-mCherry
FEATURES             Location/Qualifiers
     source          1..9712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1747..1812
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    1822..1846
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5902..5928
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             5938..6648
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6083..6101)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6351..6370
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6734..6753)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6799..6854
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6853..6872)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7215..7231)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7215..7231)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7228..7250)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7239..7255
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7263..7269,7270..7287,7288..7293))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7308..7329
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7388..7583
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7434..7569
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7589..7723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7626..7645)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7680..7699
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7791..7808)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7962..8550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8042..8061)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8721..9512,9513..9581))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9344..9363
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9582..9686)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgaggatc
     1741 accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
     1801 gacgatgaca agcaccggta tacaagtttg tacaaaaaag caggctccgc ggccgctatg
     1861 aacatccccg ctctggtgga aaaccagaag aagtactttg gcacctacag cgtgatggcc
     1921 atgctgaacg ctcagaccgt gctggaccac atccagaagg tggccgatat tgagggcgag
     1981 cagaacgaga acaacgagaa tctgtggttt caccccgtga tgagccacct gtacaacgcc
     2041 aagaacggct acgacaagca gcccgagaaa accatgttca tcatcgagcg gctgcagagc
     2101 tacttcccat tcctgaagat catggccgag aaccagagag agtacagcaa cggcaagtac
     2161 aagcagaacc gcgtggaagt gaacagcaac gacatcttcg aggtgctgaa gcgcgccttc
     2221 ggcgtgctga agatgtacag ggacctgacc aacgcataca agacctacga ggaaaagctg
     2281 aacgacggct gcgagttcct gaccagcaca gagcaacctc tgagcggcat gatcaacaac
     2341 tactacacag tggccctgcg gaacatgaac gagagatacg gctacaagac agaggacctg
     2401 gccttcatcc aggacaagcg gttcaagttc gtgaaggacg cctacggcaa gaaaaagtcc
     2461 caagtgaata ccggattctt cctgagcctg caggactaca acggcgacac acagaagaag
     2521 ctgcacctga gcggagtggg aatcgccctg ctgatctgcc tgttcctgga caagcagtac
     2581 atcaacatct ttctgagcag gctgcccatc ttctccagct acaatgccca gagcgaggaa
     2641 cggcggatca tcatcagatc cttcggcatc aacagcatca agctgcccaa ggaccggatc
     2701 cacagcgaga agtccaacaa gagcgtggcc atggatatgc tcaacgaagt gaagcggtgc
     2761 cccgacgagc tgttcacaac actgtctgcc gagaagcagt cccggttcag aatcatcagc
     2821 gacgaccaca atgaagtgct gatgaagcgg agcagcgaca gattcgtgcc tctgctgctg
     2881 cagtatatcg attacggcaa gctgttcgac cacatcaggt tccacgtgaa catgggcaag
     2941 ctgagatacc tgctgaaggc cgacaagacc tgcatcgacg gccagaccag agtcagagtg
     3001 atcgagcagc ccctgaacgg cttcggcaga ctggaagagg ccgagacaat gcggaagcaa
     3061 gagaacggca ccttcggcaa cagcggcatc cggatcagag acttcgagaa catgaagcgg
     3121 gacgacgcca atcctgccaa ctatccctac atcgtggaca cctacacaca ctacatcctg
     3181 gaaaacaaca aggtcgagat gtttatcaac gacaaagagg acagcgcccc actgctgccc
     3241 gtgatcgagg atgatagata cgtggtcaag acaatcccca gctgccggat gagcaccctg
     3301 gaaattccag ccatggcctt ccacatgttt ctgttcggca gcaagaaaac cgagaagctg
     3361 atcgtggacg tgcacaaccg gtacaagaga ctgttccagg ccatgcagaa agaagaagtg
     3421 accgccgaga atatcgccag cttcggaatc gccgagagcg acctgcctca gaagatcctg
     3481 gatctgatca gcggcaatgc ccacggcaag gatgtggacg ccttcatcag actgaccgtg
     3541 gacgacatgc tgaccgacac cgagcggaga atcaagagat tcaaggacga ccggaagtcc
     3601 attcggagcg ccgacaacaa gatgggaaag agaggcttca agcagatctc cacaggcaag
     3661 ctggccgact tcctggccaa ggacatcgtg ctgtttcagc ccagcgtgaa cgatggcgag
     3721 aacaagatca ccggcctgaa ctaccggatc atgcagagcg ccattgccgt gtacgatagc
     3781 ggcgacgatt acgaggccaa gcagcagttc aagctgatgt tcgagaaggc ccggctgatc
     3841 ggcaagggca caacagagcc tcatccattt ctgtacaagg tgttcgcccg cagcatcccc
     3901 gccaatgccg tcgagttcta cgagcgctac ctgatcgagc ggaagttcta cctgaccggc
     3961 ctgtccaacg agatcaagaa aggcaacaga gtggatgtgc ccttcatccg gcgggaccag
     4021 aacaagtgga aaacacccgc catgaagacc ctgggcagaa tctacagcga ggatctgccc
     4081 gtggaactgc ccagacagat gttcgacaat gagatcaagt cccacctgaa gtccctgcca
     4141 cagatggaag gcatcgactt caacaatgcc aacgtgacct atctgatcgc cgagtacatg
     4201 aagagagtgc tggacgacga cttccagacc ttctaccagt ggaaccgcaa ctaccggtac
     4261 atggacatgc ttaagggcga gtacgacaga aagggctccc tgcagcactg cttcaccagc
     4321 gtggaagaga gagaaggcct ctggaaagag cgggcctcca gaacagagcg gtacagaaag
     4381 caggccagca acaagatccg cagcaaccgg cagatgagaa acgccagcag cgaagagatc
     4441 gagacaatcc tggataagcg gctgagcaac agccggaacg agtaccagaa aagcgagaaa
     4501 gtgatccggc gctacagagt gcaggatgcc ctgctgtttc tgctggccaa aaagaccctg
     4561 accgaactgg ccgatttcga cggcgagagg ttcaaactga aagaaatcat gcccgacgcc
     4621 gagaagggaa tcctgagcga gatcatgccc atgagcttca ccttcgagaa aggcggcaag
     4681 aagtacacca tcaccagcga gggcatgaag ctgaagaact acggcgactt ctttgtgctg
     4741 gctagcgaca agaggatcgg caacctgctg gaactcgtgg gcagcgacat cgtgtccaaa
     4801 gaggatatca tggaagagtt caacaaatac gaccagtgca ggcccgagat cagctccatc
     4861 gtgttcaacc tggaaaagtg ggccttcgac acataccccg agctgtctgc cagagtggac
     4921 cgggaagaga aggtggactt caagagcatc ctgaaaatcc tgctgaacaa caagaacatc
     4981 aacaaagagc agagcgacat cctgcggaag atccggaacg ccttcgatgc aaacaattac
     5041 cccgacaaag gcgtggtgga aatcaaggcc ctgcctgaga tcgccatgag catcaagaag
     5101 gcctttgggg agtacgccat catgaaggga ggaccaagca gcggagcacc accaccaatg
     5161 aacaacaagt tcgacgcttt gaaagatgat gacagtgggg accatgatca gaatgaagaa
     5221 aacagcacac agaaagatgg tgagaaggaa aaaacggaac gagacaagaa tcagagcagt
     5281 agcaagagaa aggctgttgt ccctggaccg gcagagcatc ccctgcagta caactacact
     5341 ttttggtact ccaggagaac ccccggccgt cccacgagct cacagagcta tgaacagaat
     5401 atcaaacaga ttggcacctt tgcctctgtg gagcagttct ggaggtttta tagccacatg
     5461 gtacgtcctg gggacctgac aggccacagt gacttccatc tcttcaaaga aggaattaaa
     5521 cccatgtggg aggatgatgc aaataaaaat ggtggcaagt ggattattcg gctgcggaag
     5581 ggcttggcct cccgttgctg ggagaatctc attttggcca tgctggggga acagttcatg
     5641 gttggggagg agatctgtgg ggctgtggtg tctgtccgct ttcaggaaga cattatttca
     5701 atatggaata agactgccag tgaccaagca accacagccc gaatccggga cacacttcgg
     5761 cgagtgctta acctacctcc caacaccatt atggaataca aaactcacac cgacagcatc
     5821 aaaatgccag gcaggctggg cccccaaagg ctcctttttc aaaacctctg gaagccgcgg
     5881 ttgaatgtgc caggatccgg actgcctcca cttgaaagac tgacactggc ggccgctatg
     5941 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     6001 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     6061 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     6121 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     6181 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     6241 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     6301 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     6361 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     6421 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     6481 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     6541 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     6601 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaagc ggccgcactc
     6661 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     6721 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     6781 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     6841 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     6901 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     6961 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     7021 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     7081 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     7141 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc
     7201 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7261 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7321 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7381 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7441 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7501 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     7561 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa
     7621 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7681 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc
     7741 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8701 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8761 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8821 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8881 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8941 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9001 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9061 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9121 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9181 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9241 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9301 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9361 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9421 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9481 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9541 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9601 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9661 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tg
//
