LOCUS       sequence-465618-       10356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector to introduce an osTir1 expressing cassette at the 
            mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron
            system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur 
            N, Dekker J, Mirny LA, Bruneau BG
  TITLE     Targeted Degradation of CTCF Decouples Local Insulation of 
            Chromosome Domains from Genomic Compartmentalization.
  JOURNAL   Cell. 2017 May 18;169(5):930-944.e22. doi: 
            10.1016/j.cell.2017.05.004.
  PUBMED    28525758
REFERENCE   2  (bases 1 to 10356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.05"; journalName: "Cell"; date: "2017-05-18- 
            18"; volume: "169"; issue: "5"; pages: "930-944.e22"
COMMENT     Sequence Label: pEN114 -
            pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-...
FEATURES             Location/Qualifiers
     source          1..10356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..658
                     /label=chicken beta-actin promoter
     intron          659..1675
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1599..1621
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1683..1702
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1761..3485
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             3486..3527
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3618..3637)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3683..3738
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3737..3756)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4099..4115)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4123..4139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4147..4153,4154..4171,4172..4177))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4192..4213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    4273..4306
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        4370..4869
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4405..4428)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4802..4821
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        4881..4928
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4947..5546
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4947..4966)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5443..5463
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5578..5595)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5584..5808
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    5863..5896
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        complement(6722..6740)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6761..6777)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6785..6801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6809..6815,6816..6833,6834..6839))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6854..6875
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6992..7009)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7163..7751)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7243..7262)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7922..8713,8714..8782))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8545..8564
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8783..8887)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8913..9368)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9050..9071)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9262..9281
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9495..9517
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9509..9526
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9510..9526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9533..9552
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9533..9551
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      541 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
      841 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
      901 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     1021 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     1081 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     1141 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     1201 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     1261 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     1321 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     1381 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1501 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     1561 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1621 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1681 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcc tcgagaattc
     1741 tcacgcgtct gcaggatatc atgacatact tccctgagga ggtggtcgaa catattttct
     1801 cattccttcc cgcccaacga gataggaata ccgtgtccct ggtgtgcaag gtctggtatg
     1861 agatcgaacg cttgagccgc agaggtgttt ttgtgggtaa ttgttatgcc gtacgagccg
     1921 ggcgagtggc cgcccggttc ccgaacgtca gggcccttac agtgaagggc aagcctcact
     1981 ttgccgactt caatttggtg cctccggact ggggcggcta cgccgggccc tggatcgagg
     2041 ccgctgcgcg cgggtgtcat ggtctggaag aactcaggat gaaaaggatg gtggtgtcag
     2101 acgagtcact ggaactgctt gcccgcagct tcccccgatt ccgcgccctg gtgctgatca
     2161 gctgtgaagg atttagcacc gacggtctgg ctgctgtagc tagccattgt aaactgctgc
     2221 gagaactgga cctgcaggaa aatgaggtag aggatagggg acctcggtgg ctgagctgtt
     2281 tcccggatag ctgcactagc ctggtgagct tgaatttcgc ttgtatcaag ggcgaagtga
     2341 atgccgggag tttggagaga ctcgtttccc gatcccccaa cctgagatct ctgcggctga
     2401 acagatccgt ttctgtggac acgctcgcca agattttgct ccggactccc aacctggagg
     2461 atctggggac cggaaatctg accgatgatt tccagacaga gagctacttt aagctcacta
     2521 gtgctctgga aaaatgcaaa atgcttaggt cactgagcgg gttttgggac gctagccctg
     2581 tctgcctgtc ttttatatat cccctgtgtg cacagttgac cggacttaat ctgagttacg
     2641 ctccaaccct cgacgctagt gaccttacta agatgatcag ccgctgcgtg aagctccaaa
     2701 ggctctgggt actggattgt atttcagaca agggactgca agttgtggcc tcctcctgca
     2761 aagatctgca ggagctgagg gtgttccctt cagatttcta tgtggccgga tacagcgcag
     2821 tcaccgagga gggattggta gcagtgtccc tggggtgccc caaactcaat agtttgctgt
     2881 acttctgtca tcagatgacc aatgctgccc ttgttaccgt tgccaaaaac tgtccaaact
     2941 tcacaaggtt tagactgtgc atcttggagc ccggtaagcc cgacgtcgta acgtcacagc
     3001 cactggacga agggttcggt gccatagtac gcgaatgtaa gggtctccag cggctgagca
     3061 tatccggcct gctgactgac aaagtgttca tgtatattgg gaagtacgcc aagcagctgg
     3121 aaatgctgtc aatcgcattc gccggagatt cagataaagg gatgatgcac gtgatgaacg
     3181 ggtgcaagaa cctgagaaaa ctcgaaattc gcgattcccc ttttggggac gccgcactgc
     3241 tgggcaattt cgcccggtac gagacgatga ggtcactttg gatgtcaagc tgcaacgtga
     3301 ccctcaaggg atgccaagtc ctggctagta aaatgccaat gctgaatgtt gaggtcatca
     3361 acgaacgcga cggtagtaat gaaatggagg aaaaccacgg cgatctgccc aaagtggaga
     3421 aactgtacgt ctatcggacc acagctggcg cacgagatga cgctcccaat ttcgtgaaaa
     3481 ttctgggcaa gcccatcccc aaccccctgc tgggcctgga cagcacctga cccgggtaag
     3541 cggcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     3601 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     3661 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     3721 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     3781 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
     3841 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     3901 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     3961 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     4021 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc
     4081 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4141 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4201 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4261 ccagcggatc ccgaagttcc tattctctag aaagtatagg aacttcaggt ctgaagagga
     4321 gtttacgtcc agccaagcta gcttggctgc aggtcgtcga aattctaccg ggtaggggag
     4381 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     4441 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     4501 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     4561 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     4621 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     4681 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     4741 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     4801 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     4861 ggcctttcga cctgcagcct gttgacaatt aatcatcggc atagtatatc ggcatagtat
     4921 aatacgacaa ggtgaggaac taaaccatga ccgagtacaa gcccacggtg cgcctcgcca
     4981 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     5041 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     5101 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     5161 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     5221 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     5281 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     5341 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     5401 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     5461 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     5521 gcatgacccg caagcccggt gcctgagggg atcaattctc tagagctcgc tgatcagcct
     5581 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5641 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5701 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5761 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     5821 aaagaaccag ctggggctcg actagagctt gcggaaccct tcgaagttcc tattctctag
     5881 aaagtatagg aacttcatca gtcaggtaca taatggatcc ggaagagggg gaagggattc
     5941 tcccaggccc agggcggtcc tcagaagcca ggaggcagca gagaactccc agaaaggtat
     6001 tgcaacactc ccctcccccc tccggagaag ggtgcggcct tctccccgcc tactccactg
     6061 cagctccctt actgataaca actcagagcg actttgggag agcaagtgct tcctgcctcc
     6121 aaaacagccc aactgagccc tcgctccttc cctccactcc ccggagtgcg cgatggaggt
     6181 ctggctcagc acgcccctct tgaggcaact caagtcggaa acgtgcttgc acccgccccg
     6241 cagccgctca gccctactgc ccgtccccgc ccccagcgcg cgcttcctgc cacgttgcgc
     6301 aggggcgcgc ggccagactc tgcggcgcgg ggccgagggg agggccggaa cctgggagcg
     6361 cctcctcgcc gcccccgctg gccggcggat ggactcaact tgcacgaaca cgagccaatg
     6421 gcaagggcca gttttctggg ccccgagagc caatcagacg acgaggcccg gccggcggcg
     6481 ggtaaaacga ctcccccaga ggaaggggag ggtgggcggc cgctcgcgcg cgagctactt
     6541 tcgctgaccc tccctcccct cccccgcccc gccagaggcc gaccgcgccc gcacgtccag
     6601 ctcgcctcac cccacctacc tcccgcccca cccagtgggc agagcgaggc tgccggcggc
     6661 tgcgcactcc ggctgccgtt aactggcggc cgccaccgcg gtggagctcc agcttttgtt
     6721 ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6781 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6841 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6901 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6961 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7021 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7081 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7141 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7201 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7261 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7321 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7381 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7441 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7501 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7561 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7621 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7681 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7741 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7801 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7861 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7921 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7981 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8041 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8101 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8161 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8221 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8281 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8341 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8401 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8461 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8521 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8581 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8641 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8701 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8761 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8821 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8881 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     8941 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     9001 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     9061 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     9121 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     9181 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     9241 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     9301 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     9361 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     9421 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     9481 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     9541 ctcactatag ggcgaattgg gtaccataaa tggagaaaaa ggagagaggc attcatggga
     9601 gtggaaagtt aagccttagg ttggattctc aatacatcta ttgttgtaaa actccctgga
     9661 ctgagaatag gcccaaatgt ggaacaccac ctgacgggag aggtgataga cactgaaaat
     9721 taaggatcaa ggcaaaggat caacaaaaag tgtactaagg agttataaaa gaactgcagt
     9781 gttgaggccc cagctacagc ctcgatttgt ggtgtatgta actaatctgt ctggtttcat
     9841 gagtcatcag acttctaaga tcaggaaggg gaaaatgcca atgctctgtc taggggttgg
     9901 ataagccagt ctaataaatg aaaatggggc taaaatgagt gttctaaaat accttttgat
     9961 aaggctgcag aaggagcggg agaaatggat atgaagtact gggctcttta aaaatgatta
    10021 aaattctgct tacatagtct aactcgcgac actgtaattt catactgtag taaggatctc
    10081 aagcaggaga gtataaaact cgggtgagca tgtctttaat ctacctcgat ggaaaatact
    10141 ccgaggcgga tcacaagcaa taataacctg tagttttgct gcataaaacc ccagatgact
    10201 acctatcctc ccattttcct tatttgcccc tattaaaaaa cttcccgaca aaaccgaaaa
    10261 tctgtgggaa gtcttgtccc tccaatttta cacctgttca attcccctgc aggacaacgc
    10321 ccacacacca ggttagcctt taagcctctc gaggtc
//
