LOCUS       sequence-465661-        5258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  10X V5 tag multimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5258)
  TITLE     Epitope tag multimer plasmids
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUC57KAN-10XV5
FEATURES             Location/Qualifiers
     source          1..5258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1384..1403
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             1637..1678
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1691..1732
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1745..1786
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1799..1840
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1853..1894
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1925..1966
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1979..2020
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2033..2074
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2087..2128
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2141..2182
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3082..3098)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3082..3098)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3095..3117)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3106..3122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3130..3136,3137..3154,3155..3160))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3175..3196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3313..3330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3484..4072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3564..3583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4243..5058)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4243..5058)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4966..4985
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(5059..5163)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5231..5249
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accagatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt caaaagtcat acatacacaa
      421 aaggacacgc acccacacag acccatcata gaaaccaatt tgaaattctg aatcctgctt
      481 gacgctcgtt aataaatgcc tggagcagtt tttaatgatt gctgagcttc tgtcactcat
      541 cccgtaaaat gtgtttccgg atttttgcag actgtgttca tgaactggac ggtcgccgtg
      601 gagagccatg tggactcgtc cttcttctgg ggctatctgg tgacgcagat tcccggcggc
      661 ttcatcgcct ccaagttccc ggccaacaag atattcggac tgtccatcgt gagctccgcc
      721 acgctgcatc tcttcgtgcc attcgccatg accctgatgc acggtcatgt ggtgatttgc
      781 gtgagggtcc tgcaaggact cttcgaggta tgtagcccaa ccaacctaac aacttgccca
      841 attcgatttt gccacagatc aaagccgggg attacggctg cgtgatgttg cgaaaatggg
      901 gagggttaga ttttgcttta tgataaagca aatatgtgaa atggaaacat gtcctaagcc
      961 ctcaaaggga tgggggtcct gggagggcag gaaaaaataa caaaagcatt tattttaatg
     1021 cgatttcttt gtgccaagga ctgggaagta tgtcattttt cttcgaatcg gaagggagag
     1081 aatgatactt tgtaaaaagg atgtttgtct attcgagttc cgaagtaact atattttaga
     1141 tatatatttt taatttcaca aagcacattt tcttaagata ctataactgc atgagactaa
     1201 gtgcagagtt tcattaagga ataactaatt ccctaatgaa actcgcgtcg cgccacttca
     1261 acgctcgatg ggagcgtcag cgtcgcgcca cttcaacgct cgatgggagc gtcattggtg
     1321 ggcggggtaa ccgtcgaaat cagtgtttag cccgggcagc atttttttca ctgcattcta
     1381 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccca aactcatcaa
     1441 tgtatcttat catgtctgga tcgagtttca ttaaggaata actaattccc taatgaaact
     1501 cttggaatat aggacaatat cctaattgac ttatttaaat aatccattcc tgcaggtgcc
     1561 gtcctacgat ccgcagggat accagcagca gggctccggc ggctggcgcg cccttaagct
     1621 cgagggctcc ggcggcggca agcccatccc caacccctta ctgggcctgg attccaccgg
     1681 ctccggcggc ggcaagccca tccccaaccc cttactgggc ctggattcca ccggctccgg
     1741 cggcggcaag cccatcccca accccttact gggcctggat tccaccggct ccggcggcgg
     1801 caagcccatc cccaacccct tactgggcct ggattccacc ggctccggcg gcggcaagcc
     1861 catccccaac cccttactgg gcctggattc caccggctcc ggcggcgtcg agggctccgg
     1921 cggcggcaag cccatcccca accccttact gggcctggat tccaccggct ccggcggcgg
     1981 caagcccatc cccaacccct tactgggcct ggattccacc ggctccggcg gcggcaagcc
     2041 catccccaac cccttactgg gcctggattc caccggctcc ggcggcggca agcccatccc
     2101 caacccctta ctgggcctgg attccaccgg ctccggcggc ggcaagccca tccccaaccc
     2161 cttactgggc ctggattcca ccggctccgg cggcgtcgac ggatccgcgg ccgcataatg
     2221 ggttcgcgat aggtcactga tatatatcat agcttttagt tgtagtcgga aatcgtttga
     2281 cgtttattgt ttgttctcgg tgtgccgtta aagttttccc ccctttttct ctctatttgt
     2341 tgagttccat cgatggacgg atccgtggac ctttcagaag tatataaata tataactaaa
     2401 gagtaacgaa tttgtcagcg aataagggga ttccacatgc cacactaaca caatgaaact
     2461 atgtacctaa cagagaaact acatatgcat acacagcaat accgataacc actaccaata
     2521 ccattagccc acatagaccc aaactggcct aatcgatcta taaagcatat atagtatata
     2581 ctattagggt ctcgcttacg cgaatttcta tttacaagct ccgaaatcga aactgcaaca
     2641 caagataggg aaagaactgg aaatatatac tcaacacttg tgtatatcat aaatcacata
     2701 atccacacaa agcctatgat aattatattt atatcggtat aatcgtatat acatatatag
     2761 ccaaagattg ttgttgaact gcgaactgca agttttatta ccgttgatga tattatcttc
     2821 tgtgtaaaga gaacatttta atcaaaccca agcaattgtt gatgttcggc acgaatgata
     2881 accataaaaa gaactagcta atcaaaagtc atatgaatac tctgttaact aattactgtt
     2941 tatgtttgtt gatattattt aattcattga tattaatgca ccgtatgctt ttgtgtttaa
     3001 ctaacatgag caaggcatga tgtaggtact aaaaataata cttaagtaga tagccgtaga
     3061 agcttggctc gatcgagcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3121 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3181 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3241 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3301 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3361 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3421 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3481 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3541 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3601 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3661 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3721 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3781 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3841 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3901 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3961 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4021 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4081 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4141 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4201 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttagaaaa actcatcgag
     4261 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     4321 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     4381 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     4441 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     4501 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     4561 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
     4621 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     4681 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     4741 tgctgttttc ccagggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     4801 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     4861 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     4921 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     4981 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc
     5041 ccgttgaata tggctcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5101 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5161 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     5221 aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
