LOCUS       sequence-465682-        8987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating targeting inversions. N-terminal split GFP, expressed
            in eyes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 8987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT22
FEATURES             Location/Qualifiers
     source          1..8987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     139..158
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(298..421)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    1427..1548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1464..1483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1518..1537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    1580..1703
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1726..1731,1732..1749,1750..1756)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1810..2469
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1876..1895)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        2478..2923
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(2924..3010,3011..4345)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(4384..4507)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4599..4618)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4600..4618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5071..5862
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6108..6696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6597..6616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6850..6867
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        6899..6917
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6899..6916
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        6994..7414
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    7421..7544
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7567..7572,7573..7590,7591..7597)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        7640..7744
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7745..7813,7814..8605)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7963..7982)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgtttgtgga tgtacagagt gatattatcg atacaccggg ccgtcgcatg gttattccac
       61 ttgcgtctgc gcgcttgctc tcagataaag tctcccgtga actttacccg gtggtgcata
      121 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtttccgtta
      181 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
      241 tgatgttctg gggaatataa tgtcaggctc ccttatacac agccagtctg cacgtcgcat
      301 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa
      361 tttaatatat tgatatttat atcattttac gtttctcgtt caacttttct atacaaagtt
      421 gtccagctgg agcgagaccg gagcaatggt taattcgagc tcgcccgggg atctaattca
      481 attagagact aattcaatta gagctaattc aattaggatc caagcttatc gatttcgaac
      541 cctcgaccgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa
      601 caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
      661 agctaaacaa tcggatgagc aagggcgagg agctgttcac cggcgtggtg cccatcctgg
      721 tggagctgga tggcgatgtg aacggccaca agttcagcgt gcgcggcgag ggcgagggcg
      781 atgccaccat cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      841 cctggcccac cctggtgacc accctgacct acggcgtgca gtgcttcagc cgctactccg
      901 atcacatgaa gcgccacgat ttcttcaaga gcgccatgcc cgagggctac gtgcaggagc
      961 gcaccatcag cttcaaggat gatggcaagt acaagacccg cgccgtggtg aagttcgagg
     1021 gcgataccct ggtgaaccgc atcgagctga agggcaccga tttcaaggag gatggcaaca
     1081 tcctgggcca caagctggag tacaacttca acagccacaa cgtgtacatc accgccgata
     1141 agcagaagaa cggcatcaag gccaacttca ccgtgcgcca caacgtggag gatggcagcg
     1201 tgcagctggc cgatcactac cagcagaaca cccccatcgg cgatggcccc gtgctgctgc
     1261 ccgataacca ctacctgagc acccagaccg tgctgagcaa ggatcccaac gagaagtaaa
     1321 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     1381 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     1441 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1501 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaaa gcttatcgat
     1561 acgcccatgg tctcaccatc aactttgtat acaaaagttg aacgagaaac gtaaaatgat
     1621 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     1681 acacaacata tccagtcact atgggccgca ttaggcaccc caggctttac actttatgct
     1741 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
     1801 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     1861 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     1921 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     1981 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     2041 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     2101 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     2161 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     2221 tttattgaga atatgttttt cgtgtcagcc aatccctggg tgagtttcac cagttttgat
     2281 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     2341 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     2401 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     2461 ggggcgtaaa cgcgtggcac atatacctgc cgttcactat tatttagtga aatgagatat
     2521 tatgatattt tctgaattgt gattaaaaag gcaactttat gcccatgcaa cagaaactat
     2581 aaaaaataca gagaatgaaa agaaacagat agatttttta gttctttagg cccgtagtct
     2641 gcaaatcctt ttatgatttt ctatcaaaca aaagaggaaa atagaccagt tgcaatccaa
     2701 acgagagtct aatagaatga ggtcgaaaag taaatcgcgc gggtttgtta ctgataaagc
     2761 aggcaagacc taaaatgtgt aaagggcaaa gtgtatactt tggcgtcacc ccttacatat
     2821 tttaggtctt tttttattgt gcgtaactaa cttgccatct tcaaacagga gggctggaag
     2881 aagcagaccg ctaacacagt acataaaaaa ggagacatga acgatgaaca tcaaaaagtt
     2941 tgcaaaacaa gcaacagtat taacctttac taccgcactg ctggcaggag gcgcaactca
     3001 agcgtttgcg aaagaaacga accaaaagcc atataaggaa acatacggca tttcccatat
     3061 tacacgccat gatatgctgc aaatccctga acagcaaaaa aatgaaaaat atcaagttcc
     3121 tgaattcgat tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg acgtttggga
     3181 cagctggcca ttacaaaacg ctgacggcac tgtcgcaaac tatcacggct accacatcgt
     3241 ctttgcatta gccggagatc ctaaaaatgc ggatgacaca tcgatttaca tgttctatca
     3301 aaaagtcggc gaaacttcta ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag
     3361 cgacaaattc gatgcaaatg attctatcct aaaagaccaa acacaagaat ggtcaggttc
     3421 agccacattt acatctgacg gaaaaatccg tttattctac actgatttct ccggtaaaca
     3481 ttacggcaaa caaacactga caactgcaca agttaacgta tcagcatcag acagctcttt
     3541 gaacatcaac ggtgtagagg attataaatc aatctttgac ggtgacggaa aaacgtatca
     3601 aaatgtacag cagttcatcg atgaaggcaa ctacagctca ggcgacaacc atacgctgag
     3661 agatcctcac tacgtagaag ataaaggcca caaatactta gtatttgaag caaacactgg
     3721 aactgaagat ggctaccaag gcgaagaatc tttatttaac aaagcatact atggcaaaag
     3781 cacatcattc ttccgtcaag aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc
     3841 tgagttagca aacggcgctc tcggtatgat tgagctaaac gatgattaca cactgaaaaa
     3901 agtgatgaaa ccgctgattg catctaacac agtaacagat gaaattgaac gcgcgaacgt
     3961 ctttaaaatg aacggcaaat ggtacctgtt cactgactcc cgcggatcaa aaatgacgat
     4021 tgacggcatt acgtctaacg atatttacat gcttggttat gtttctaatt ctttaactgg
     4081 cccatacaag ccgctgaaca aaactggcct tgtgttaaaa atggatcttg atcctaacga
     4141 tgtaaccttt acttactcac acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat
     4201 tacaagctat atgacaaaca gaggattcta cgcagacaaa caatcaacgt ttgcgccaag
     4261 cttcctgctg aacatcaaag gcaagaaaac atctgttgtc aaagacagca tccttgaaca
     4321 aggacaatta acagttaaca aataaatgtc aggctccctt atacacagcc agtctgcacg
     4381 tcgcatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     4441 atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac tttttaatac
     4501 aaagttgcct ccggaacata atggtgcatc tagatgccca ttcctgtaga cttcttaatt
     4561 aagacgtcag aattctcgag gcggccgcat gtgagtctcc ctatagtgag tcgtattaat
     4621 ttcgcgggcg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     4681 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     4741 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga
     4801 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     4861 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     4921 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     4981 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     5041 ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg
     5101 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     5161 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     5221 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     5281 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     5341 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     5401 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     5461 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     5521 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     5581 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     5641 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     5701 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     5761 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     5821 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg
     5881 ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat
     5941 aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa
     6001 taagccctac acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt
     6061 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6241 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6721 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6781 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6841 accgagcgca gcgagtcagt gagcgaggaa atcgtcacct gcaggaaggt ttaaacgcat
     6901 ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc gaattcgaag tcttggtacg
     6961 gaggggatcc gatcgacaag ccgaacatat gggttttttg ctcacctgtg attgctccta
     7021 ctcaaataca aaaacatcaa attttctgtc aataaagcat atttatttat atttatttta
     7081 caggaaagaa ttccttttaa agtgtatttt aacctataat gaaaaacgat taaaaaaaat
     7141 acataaaata attcgaaaat ttttgaatag cccaggttga taaaaattca tttcatacgt
     7201 tttataactt atgcccctaa gtattttttg accatagtgt ttcaattcta cattaatttt
     7261 acagagtaga atgaaacgcc acctactcag ccaagaggcg aaaaggttag ctcgccaagc
     7321 agagagggcg ccagtgctca ctacttttta taattctcaa cttctttttc cagactcagt
     7381 tcgtatatat agacctattt tcaatttaac gtcggggcct caactttgta taataaagtt
     7441 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     7501 cagactacat aatactgtaa aacacaacat atccagtcac tatgggccgc attaggcacc
     7561 ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg
     7621 tcgagatttt caggagctac gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7681 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7741 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7801 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7861 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7921 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7981 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8041 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8101 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8161 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8221 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8281 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8341 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8401 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8461 gtcccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8521 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8581 tgcctcactg attaagcatt ggtaatggat ccggcttact aaaagccaga taacagtatg
     8641 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
     8701 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
     8761 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
     8821 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
     8881 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga
     8941 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtc
//
