LOCUS       sequence-465702-       12940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli-C. difficile shuttle vector for CRISPR editing in C. 
            difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA
            for homology directed repair. MscI and NotI for replacement of 
            sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12940)
  AUTHORS   Bollinger KW, Muh U, Ocius KL, Apostolos AJ, Pires MM, Helm RF, 
            Popham DL, Weiss DS, Ellermeier CD
  TITLE     Identification of a family of peptidoglycan transpeptidases reveals 
            that Clostridioides difficile requires noncanonical cross-links for 
            viability.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2408540121. doi: 
            10.1073/pnas.2408540121. Epub 2024 Aug 16.
  PUBMED    39150786
REFERENCE   2  (bases 1 to 12940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-20"; volume: "121(34):e2408540121. 
            doi"; pages: " 10.1073/pnas.2408540121. Epub 2024 Aug 16"
COMMENT     Sequence Label: pIA123
FEATURES             Location/Qualifiers
     source          1..12940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1525..5631
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1525..5628
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        6525..6600
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(6995..7366)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            7399..7508
                     /note="incP origin of transfer"
     primer_bind     complement(8704..8721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8875..9463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8955..8974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acatctcccc taatttttag acttaagggc gaattctgca tcaagctagc ataaaaataa
       61 gaagcctgca tttgcaggct tcttattttt atggtacgta taatttaaag ggttatggct
      121 gtatatagtc ataaccctta tatttcatta attaaagtta aattttgtat attcaaaaag
      181 ctcttgatat ttacggcaga gccaccatat agagatgcct cagaccctaa tgatgaaatt
      241 tctatattta ttcctttgtt aaaagagcta actaacatat cttttgtaat ctgtaatatc
      301 tcagggatat cactaattat ttggctattt aaatatatta tttcaggagc ataagttgta
      361 atagcattat ttatagctat tgttaagtaa ctacaaaact catgaataac ttttttggca
      421 ttctggttat cttcataata tagttgcttt actatatcag aatctatttt aggaatattt
      481 tctaaagaag aaagctgttc aaataccttt ttttctgaac aatactgttc taaacagcca
      541 cgatttccac aaggacatag tttaccatta ggcatgatga tagtgtgacc aatttctcca
      601 ctcataccat ttctgccact atataattta ttatttatta taatgcctga accaaatcca
      661 ctgtgaatac tgagactaag tagtgagtta tgtacagttg aaaaagtatt ctcagctaaa
      721 gctgttaagt ttgcttcatt ttctatgtga ataggaaagt catacttttt gctaaggatg
      781 ctatataagt caatctcatt aagattgtaa tatggagtaa acaatacttt attttcacaa
      841 gtaatcccat gtatagccaa agtaagacca attaccttat aaggagtgtc tattttggat
      901 atgttataac tatttataat ttcatctatc aattgtataa cattgtcttt acttacttgt
      961 atatctgtta atttcttaga atttataata gttccatcta agtaagatag agaagaaaat
     1021 atatagtcgt atccaatgtc catacttaaa gaaatccctg cacatttatt aaatactaat
     1081 aatatgggtt ttcttccgcc actatgagta ctatttccaa ttcctatttc atgaactaga
     1141 gattcatcaa taagtttttt ggtaatagcg gatatagttg ctttattcaa tcctatagta
     1201 gaagctatac ttgccctaga aataggacca ttttttataa tttgttcaag taccaatctt
     1261 tcattcattt ctcgaatagt gtatttatca gtaaccaatt tgatattcct ccttaaaata
     1321 atattgtaat actttttaca caaaaataaa aggttatttt gcattgacaa agataattaa
     1381 atattttatt attagttcat aagttagttt aatatactaa caaaaataaa gcaagtaaaa
     1441 tatacctaaa atataaaaaa attaggatag gaaaacgata gttatgaagt ggcattcaag
     1501 gagggtaaag aggagagtcg acgcatggat aaaaaatata gtataggatt agatatagga
     1561 acaaatagtg taggatgggc agtaataact gatgaatata aagtaccaag taaaaaattt
     1621 aaagtattag gaaatacaga tagacatagt ataaaaaaaa atttaatagg tgcattactt
     1681 tttgatagtg gtgaaacagc agaagcaaca agacttaaaa gaacagcaag aagaagatat
     1741 actagaagaa aaaatagaat atgttattta caagaaatat ttagtaatga aatggcaaaa
     1801 gtagatgata gtttttttca tagattagaa gaaagttttt tagtagaaga agataaaaaa
     1861 cacgaaagac atccaatatt tggaaatata gtagatgaag tagcatatca tgaaaaatat
     1921 ccaacaatat atcatttaag aaaaaaactt gtagatagta cagataaagc agatttaaga
     1981 cttatatatc ttgcattagc acatatgata aaatttagag gacattttct tatagaaggt
     2041 gatttaaatc cagataatag tgatgtagat aaacttttta tacaattagt acaaacatat
     2101 aatcaattat ttgaagaaaa tccaataaat gcaagtggtg tagatgcaaa agcaatatta
     2161 agtgcaagat taagtaaaag tagaagatta gaaaatctta tagcacaatt acctggagaa
     2221 aaaaaaaatg gattatttgg taatcttata gctttaagtt taggattaac accaaatttt
     2281 aaatcaaatt ttgatcttgc agaagatgca aaacttcaat taagtaaaga tacatatgat
     2341 gatgatttag ataatttact tgcacaaata ggtgatcaat atgcagattt atttcttgca
     2401 gcaaaaaatc tttctgatgc aatactttta agtgatatac ttagagtaaa tacagaaata
     2461 acaaaagcac cattatctgc aagtatgata aaaagatatg atgaacatca tcaagattta
     2521 acattactta aagcattagt aagacaacaa ttaccagaaa aatataaaga aatttttttt
     2581 gatcaaagta aaaatggata tgcaggatat atagatggtg gtgcaagtca agaagaattt
     2641 tataaattta ttaaaccaat attagaaaaa atggatggaa cagaagaatt attagtaaaa
     2701 cttaatagag aagatttact tagaaaacaa agaacatttg ataatggaag tataccacat
     2761 caaatacatt taggtgaatt acatgcaata ttaagaagac aagaagattt ttatccattt
     2821 cttaaagata atagagaaaa aattgaaaaa atacttacat ttagaatacc atattatgta
     2881 ggaccattag caagaggaaa tagtagattt gcttggatga caagaaaaag tgaagaaaca
     2941 ataacaccat ggaattttga agaagtagta gataaaggag catctgcaca aagttttata
     3001 gaaagaatga caaattttga taaaaatctt ccaaatgaaa aagtattacc aaaacatagt
     3061 ttattatatg aatattttac agtatataat gaattaacaa aagtaaaata tgtaacagaa
     3121 ggtatgagaa aaccagcatt tttaagtggt gaacaaaaaa aagctatagt agatttatta
     3181 tttaaaacaa atagaaaagt aacagtaaaa caattaaaag aagattattt taaaaaaatt
     3241 gaatgttttg atagtgtaga aatatctggt gtagaagata gatttaatgc aagtttagga
     3301 acatatcatg atttacttaa aataataaag gataaagatt ttttagataa tgaagaaaat
     3361 gaagatattt tagaagatat agtattaaca ttaactttat ttgaagatag agaaatgata
     3421 gaagaaagat taaaaactta tgctcattta tttgatgata aagtaatgaa acaacttaaa
     3481 agaagaagat atacaggttg gggaagatta agtagaaaac ttataaatgg aataagagat
     3541 aaacaaagtg gaaaaacaat attagatttt cttaaaagtg atggatttgc aaatagaaat
     3601 tttatgcaat taatacatga tgatagttta acatttaaag aagatattca aaaagcacaa
     3661 gtaagtggac aaggtgatag tttacatgaa catatagcaa atttagcagg atctccagca
     3721 ataaaaaaag gtatattaca aacagtaaaa gtagttgatg aattagtaaa agtaatgggt
     3781 agacataaac cagaaaatat agttatagaa atggcaagag aaaatcaaac aacacaaaaa
     3841 ggacaaaaaa atagtagaga aagaatgaaa agaatagaag aaggtataaa agaattagga
     3901 agtcaaatac ttaaagaaca tccagtagaa aatacacaac ttcaaaatga aaaactttat
     3961 ttatattatt tacaaaatgg aagagatatg tatgtagatc aagaattaga tataaataga
     4021 ttaagtgatt atgatgtaga tcatatagta ccacaaagtt ttcttaaaga tgattctata
     4081 gataataaag tattaacaag atctgataaa aatagaggta aaagtgataa tgtaccaagt
     4141 gaagaagttg taaaaaaaat gaaaaattat tggagacaat tacttaatgc aaaacttata
     4201 acacaaagaa aatttgataa tcttacaaaa gcagaaagag gtggattatc tgaattagat
     4261 aaagctggat ttataaaaag acaattagta gaaactagac aaataacaaa acatgtagca
     4321 caaatattag atagtagaat gaatacaaaa tatgatgaaa atgataagct tataagagaa
     4381 gtaaaagtta taacattaaa aagtaaatta gtaagtgatt ttagaaaaga ttttcaattt
     4441 tataaagtaa gagaaataaa taattatcat catgcacatg atgcatatct taatgcagta
     4501 gtaggaacag cattaataaa aaaatatcct aaattagaat ctgaatttgt atatggagat
     4561 tataaagtat atgatgtaag aaaaatgata gcaaaaagtg aacaagaaat aggaaaagca
     4621 acagcaaagt acttttttta ttctaatata atgaattttt ttaagacaga aataacttta
     4681 gcaaatggtg aaataagaaa aagaccttta atagaaacta atggtgaaac aggtgaaata
     4741 gtatgggata aaggtagaga ttttgcaaca gtaagaaaag tattaagtat gccacaagta
     4801 aatatagtaa aaaaaacaga agtacaaact ggaggattta gtaaagaaag tatacttcca
     4861 aaaagaaatt cagataaact tattgcaaga aaaaaagatt gggatccaaa aaaatatggt
     4921 ggatttgata gtccaacagt agcatatagt gtattagtag tagcaaaagt agaaaaaggt
     4981 aaatctaaaa aacttaaaag tgtaaaagaa ttacttggaa taacaataat ggaaagaagt
     5041 agttttgaaa aaaatcctat tgatttttta gaagcaaaag gatataaaga agttaaaaaa
     5101 gatttaataa taaaacttcc aaaatattct ttatttgaat tagaaaatgg aagaaaaaga
     5161 atgttagcaa gtgctggtga attacaaaaa ggtaatgaat tagcattacc atctaaatat
     5221 gtaaattttc tttatttagc aagtcattat gaaaaactta aaggatctcc tgaagataat
     5281 gaacaaaaac aattatttgt agaacaacat aaacattatt tagatgaaat aattgaacaa
     5341 ataagtgaat tttctaaaag agtaatatta gcagacgcaa atttagataa agtattatct
     5401 gcttataata aacatagaga taaaccaata agagaacaag cagaaaatat aatacatctt
     5461 tttacattaa caaatttagg tgcaccagca gcatttaaat attttgatac aacaatagat
     5521 agaaaaagat atacaagtac aaaagaagta ttagacgcaa cattaataca tcaaagtata
     5581 acaggattat atgaaacaag aatagattta tctcaattag gtggtgatta actcgagcaa
     5641 aaacatcgta gaaatacggt gttttttgtt accctaagtt tcataaaaat aagaagcctg
     5701 caaatgcagg cttcttattt ttatggttta aacggtttta gctgggatat cggcaaaaca
     5761 agcaaatact actatagaag cagttaaaag ggctattttt tataatctgg agcatagaca
     5821 agaagttaga gaagcaatta aaaaagaaaa tataagaaaa attcttatta ttgggacatc
     5881 aaaaaaaatg gttaatcaaa ttgtagagag attgtcaatt ggaagggtct gtaagtttat
     5941 aaatatacaa gatataagta ctaaatatga aatagaaata gcaaaacaag ctagaaaaga
     6001 aggaaatcat gtaattcctg ttccagccgt tgagataaaa agtatggcaa gtggtctcag
     6061 tataaattct ttgaagagac tttttagaaa aggaaataat aggaatatgg tagttttaga
     6121 aaaaacaatt ataagaccaa cttttagtta tataggaaag ttttatatta gtgctgatgt
     6181 aattaaacag attattgaat atgaagtata caactttgga agcattgata gaataaataa
     6241 aatcaatata gagaattgca ataattttat gaatattttt gtaagtataa atatcaatga
     6301 tttagaaact ataaaaaaaa ttgaagacat acaacagaca ataaaaaagt ctattgagaa
     6361 aatgacttta gttaatgtac aaaaaataga tattaacatt cataaaatta aaaaaataaa
     6421 cttcctccaa tgttaaaaaa taaactgaga aaatgatata ctaatttgca ataaagtgtg
     6481 ctataattaa actgtaaatg gccactgcta ttgaaacacc aacagtttta gagctagaaa
     6541 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     6601 tttttttcta tggagaaatc tagatcagca tgatgtctga ctagcggccg cggatcctat
     6661 aagttttaat aaaactttaa atagaaaaag gcttctctca tgagaagtct tttttattta
     6721 aaataaatat aaaataaaat agaggctata aatagcctct attttatgtg agaaatccct
     6781 aaataaaaag atgccagtgt gctggaattc gcccttaggc tgcgctaggg acctctttag
     6841 ctccttggaa gctgtcagta gtaggggatc ggtcttgcct tgctcgtcgg tgatgtactt
     6901 caccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac
     6961 gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca
     7021 cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga
     7081 ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca
     7141 ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt
     7201 ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca
     7261 tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct
     7321 tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat
     7381 ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca
     7441 ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta
     7501 tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa
     7561 atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga
     7621 agcaggaagg gcgaattctg cagtgcagat ctgaccggtc tctgaaaata taaaaaccac
     7681 agattgatac taaaaccttg gttgtgttgc ttttcggggc ttaaatcaag gaaaaatcct
     7741 tgttttaagc ctttcaaaaa gaaacacaag gtctttgtac taacctgtgg ttatgtataa
     7801 aattgtagat tttagggtaa caaaaaacac cgtatttcta cgatgttttt gcttaaatac
     7861 ttgtttttag ttacagacaa acctgaagtt aactatttat caattcctgc aattcgttta
     7921 caaaacggca aatgtgaaat ccgtcacata ctgcgtgatg aacttgaatt gccaaaggaa
     7981 gtataatttt gttatcttct ttataatatt tccccatagt aaaaatagga atcaaataat
     8041 catatccttt ctgcaaattc agattaaagc catcgaaggt tgaccacggt atcatagata
     8101 cattaaaaat gttttccgga gcatttggct ttccttccat tctatgattg tttccatacc
     8161 gttgcgtatc actttcataa tctgctaaaa atgatttaaa gtcagactta cactcagtcc
     8221 aaaggctgga aaatgtttca gtatcattgt gaaatattgt atagcttggt atcatctcat
     8281 catatatccc caattcacca tcttgattga ttgccgtcct aaactctgaa tggcggttta
     8341 caatcattgc aatataataa agcattgcag gatatagttt cattcccttt tcctttattt
     8401 gtgtgatatc cactttaacg gtcatgctgt aggtacaagg tacacttgca aagtagtggt
     8461 caaaatactc ttttctgttc caactatttt tatcaatttt ttcaaatacc atctaagttc
     8521 cctctcaaat tcaagtttat cgctctaatg aacaaagata ttataccaca tttttgtgaa
     8581 tttttcaact tgcccacttc gactgcactc ccgacttaat aacttcttga acacttgccg
     8641 aaaaagaaaa actgccgggt acgtacccgg ggatcgatcc ccggccgagc gctcttccgc
     8701 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8761 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8821 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8881 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8941 cccgacagga ctataaagat accaggcgtt tcccccttga agctccctcg tgcgctctcc
     9001 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9061 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9121 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9181 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9241 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9301 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9361 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9421 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9481 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa
     9541 atgcaagttt ctaactaaca tacatcatag ttactaaact atggtaacta taattttatt
     9601 aactatatta agcactgatt agtactataa ctcaatataa gcatatcccc tgtatcgtaa
     9661 ctagagaacc aaacgacgga aaaagcgata tagataagtt tagatagtgc accaattttt
     9721 tacttttcta ttttagaatc tagcatttcc aatgcttact aatactattt ttgattttta
     9781 acttgtttta aaaaagcacc tactaattag gattctagct ctatattcta gttatacatt
     9841 ctctatattc tagctctata ttctagttat acattctcta tattctagct ctatattcta
     9901 gttatacatt ctctatattc tagctctata ttctagttat acattctcta tattctagct
     9961 ctatattcta gttatacatt ctctatattc tagctctata ttctagttat acattctcta
    10021 tattctagct ctatattcta gttatacatt ctctaaaaaa catatatata tatactacat
    10081 atatatgtac aattatttta ccaaaagaaa tttttaaaac ggcttaattt caatactttg
    10141 atgccatttt ttttttgcaa ttctcgtaca attattttac caaaagaaat ttttattaat
    10201 tagttttttt gcataaaaat aggactagaa aatcaattct aatcctatca aaaatctact
    10261 tgtttaatct atatgtatag tacataaatc ctctatcatt tattttagtt tcccatacat
    10321 ctaaaatttt agtatctaat aaagattcta tcatatttga aattctttgc tgtgttactc
    10381 ctaaatcttt tgctaattcc tcttgattaa ttcttaatat atttcctttt attttatcat
    10441 ctttgaccaa taaatcttgc ttatatctca tatgagtata taatcttaat tcttcagttg
    10501 aaattattcc ccaaattact gccatggtaa caaagtaact aatagtatat tgtttatcta
    10561 tattacatct aacttctttt acattgtatg tatcttttat tcctagcttt gtaacacctt
    10621 ttgtttttgt aatataatca ttatgttgaa gttcttttaa tgtttctctt aatgtctttt
    10681 cactcataac aatgttttta actttcttct tacgtttata ggttatcagc tccattatat
    10741 cgtctatgtt taattctcta tctttattgt taagtaacac attatagata aacaattgat
    10801 taccagtcat tattttaacc cctttcctat tcttatattt caaatccttt gagtgcttat
    10861 gtggcatatt gaacaaagta tcttcatcac tatatataaa atctgattct attttattcc
    10921 aacaatcact attaaactca caacctgtac aaccaaaatt aaccgtttta agcttctcat
    10981 acatatactt gacttgatat tctagctcac tatttgaaag actattttgt gatatatagt
    11041 tccattctct agcaacggat attacttgac ttaaactttt attcctcaat cttaaagtca
    11101 caactatttt ttgcaaatct aggtttttat gtccatcttt actaccattt tttaaaatat
    11161 ctactataca atttggtagc ttactatcta atatatattt tgtattagta ttacctactg
    11221 tttttacatc tttgcaatat tttttagcta acttttctat gtcatatcta tatatatttt
    11281 tgtcttggtg tattattttt acttgtttag tagtattttt aatattatat gtataaggta
    11341 ctcttaatac ttgagtagat aaatttgctt gtttatctgc acccaattta tttattaatg
    11401 tatttgtaac ttctgatact aacttaatgt tattagtttt attaattagc acataaacat
    11461 ggaatccatt tccactatca actaggatat gataatgtaa tcctatctta gtaaataaat
    11521 taattatatc tttataatta aaaccttgtc ctaattcttt tttgtcaaaa tcaaatccta
    11581 atgcatatct atttattaag ttttcattgg ttcctgcttt tccatcagtt gtacataggt
    11641 taaaaaatgt attttcataa taaatatttt ttgcgaaggt aaattctact atatcatcta
    11701 tatttttaaa aaaagttgtt ttactatatt ctttaaattc ttctccgagt tctaattgtt
    11761 tactattact tttattattt tgaaatactc taacatattc attatcaata agattatttc
    11821 cttgtcctat accatctcta aatactgcca ttagaaattt tttcaactta tattttgaat
    11881 ctaattgttc catatattta ttatctcctt atctagtttt tttaagagat aacactacat
    11941 taaatatata cttattgact aatcaacttt tacaactatt gaataatttt agcttagtat
    12001 gtataataat attgttaaga tgctaaaatt gatagcaata ttattatata actacagata
    12061 cttaatcagt tagaccgata ttggagttgg tagctccata aactcaattg gtagttgagt
    12121 tgtcattgtc tagtagtatt atctcatttt gttgttatct taaaatatta ttttaaaaat
    12181 actctgcttg gtcggcttag ggtatttttt ttgtttaatt ccaatattgg ttcaaactcc
    12241 tgtgtaatac ttttgacact tccatctgta ttgaattgac agttgatatg gtatctcatt
    12301 attggcttgt tcttatgttt ttctatgtat ttatttttta ctttcaaata atttgtattt
    12361 ccatttttag ttatgtttaa cacactatcc ttattactca ataaaattat tttttgtttt
    12421 aacatatgat ttatagaatc taaaaattga tattgactta taatagtact ttctacctct
    12481 ctagcttttt ctagtgtttc aaattcttca aactctgcta tattgctata gtatagctta
    12541 tcattaataa tattatctga caactttaat tccattttat aaaacaattc caagttatgt
    12601 ataaaatttt taatgttaca acatatagac ttattatttg ttcttatatg gtatctccat
    12661 ttactacttc tagctctcat gttatatgca caactactcc caacataaac atatattgct
    12721 tgttctaact catatttttg taaaatttca acatcatatc cagtcaaatc gtactctctc
    12781 ttatatacaa atggtttttc taattttttg ttcaataaat ctcctcctat ttcttactta
    12841 atttagctaa aaatctattt atatttcacc tcctttttga ctttaagcct acgaatacca
    12901 tatttatgat tataacatct tctatttttc ccaaatcctt
//
