LOCUS       sequence-465704-       13880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human reticulon 3 (RTN3) under 
            the spleen focus-forming virus (SFFV) promoter and Emerald under the
            Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13880)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-RTN3-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..13880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2414..2423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2881..2892)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5632..5652)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6050..6061)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7100..7119
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      7293..7302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7299..7301,7302..7304,7305..8018)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7344..7365)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7605..7624)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7952..7973
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             8113..8346
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8371..8389)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8372..8389)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8679..8698)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8912..8931)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9031..9053
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9091..9109)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9177..9281
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9282..9350,9351..10142)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9500..9519)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10313..10901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10802..10821
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11055..11072
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11142..11162)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11147..11476
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11327..11462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12610..12675
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12805..12825
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13250..13384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13287..13306)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13341..13360
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(13552..13880,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tggcggagcc gtcggcggcc actcagtccc attccatctc
     2461 ctcgtcgtcc ttcggagccg agccgtccgc gcccggcggc ggcgggagcc caggagcctg
     2521 ccccgccctg gggacgaaga gctgcagctc ctcctgtgcg gattcctttg tttcttcctc
     2581 ttcctctcag cctgtatctc tattttcgac ctcacaagag ggattgagct ctctttgctc
     2641 tgatgagcca tcttcagaaa ttatgacttc ttcctttctt tcatcttctg aaatacataa
     2701 cactggcctt acaatactac atggagaaaa aagccatgtg ttagggagcc agcctatttt
     2761 agccaaagaa ggaaaagacc acttggatct tctagatatg aaaaagatgg aaaagcctca
     2821 ggggaccagc aacaacgtat cagactcttc agtttctctt gcagcaggag ttcattgtga
     2881 ccgtccttct attccagcca gtttcccaga gcatcctgct tttctctcaa agaaaattgg
     2941 tcaagtggaa gagcaaatag ataaagagac caagaaccca aatggggtat caagtaggga
     3001 ggctaaaact gcattggatg ctgatgacag attcactttg ctgacagccc agaaaccacc
     3061 tactgagtac tctaaggtag aaggcattta tacatattct ttgtctccat ccaaagtttc
     3121 aggagatgat gttattgaaa aggattcccc tgaatcacca tttgaagtaa ttattgacaa
     3181 agcagcattt gacaaagaat ttaaagactc atataaggag agcacagatg attttggtag
     3241 ctggtctgtg cacactgata aagaatcatc cgaagacatt tcagagacta atgacaagct
     3301 ttttccactg agaaataaag aggcaggacg ttacccaatg tctgcattgc tcagtaggca
     3361 gttttcacac acaaatgcag cactggaaga ggtgtccaga tgcgtgaatg atatgcataa
     3421 ctttactaac gaaatactga cttgggatct ggttccccaa gtgaaacaac agaccgataa
     3481 atcttctgac tgcatcacaa aaactacagg acttgacatg agtgaatata attcagaaat
     3541 tccagttgta aatcttaaaa ctagcactca tcagaaaact cctgtatgtt ctattgatgg
     3601 gagcactccc atcactaaat caacaggtga ttgggcagaa gcatctctcc agcaagaaaa
     3661 tgctattact ggaaaacctg tacctgactc tttgaattcc acaaaagaat tcagtatcaa
     3721 aggtgtgcaa ggcaatatgc agaaacagga tgacacactt gcagaattac ctggatctcc
     3781 acctgagaaa tgtgactctt tgggttctgg agtggccaca gtgaaagtgg ttttacctga
     3841 tgaccacctg aaagatgaaa tggactggca gagctctgca ttgggagaaa tcacagaagc
     3901 tgatagttct ggtgagtctg atgacacagt aatagaggac atcacagcag atacatcatt
     3961 tgaaaataac aaaattcagg ctgaaaaacc tgtttccatt ccaagtgctg ttgtaaaaac
     4021 aggtgaaaga gaaatcaaag agattcccag ttgtgagaga gaagaaaaaa catctaaaaa
     4081 ctttgaagaa ttggtcagtg actctgagct gcatcaagat cagcctgata ttcttggaag
     4141 gagtccagct agtgaggcag catgttcaaa agtacccgat acgaatgtct ccttagaaga
     4201 tgtgagtgaa gttgctcctg aaaagcctat tactactgag aaccccaaac ttccttcaac
     4261 agtgtctcca aatgttttta atgagacaga attctcatta aatgtgacaa catctgccta
     4321 tttggagtca ttacatggga aaaatgttaa acatatagat gattcctccc cagaggacct
     4381 gatagcagcc tttacagaaa ccagagataa aggaatagta gatagtgaaa gaaatgcttt
     4441 taaagcaata tcagagaaga tgacagactt taaaacaact cctcctgtag aagtcttaca
     4501 tgaaaatgag tccggtggtt ctgaaattaa agacattgga agcaaataca gtgaacaaag
     4561 caaagaaaca aatggaagtg agcctctagg tgttttccct acccaaggta ctccagtagc
     4621 atctcttgac ttagaacaag aacagctcac aattaaggct cttaaagaat taggtgaaag
     4681 acaggttgag aagtcaactt ctgcacagcg tgacgcagaa ttgccttctg aagaagtact
     4741 gaagcaaact ttcacatttg ctccagaatc ttggccacag agatcatatg acatcctaga
     4801 acgtaatgtc aagaatggat ctgatcttgg gatttcccag aagcccatca ctatcagaga
     4861 aactactagg gtagatgctg tttccagcct tagcaagact gaattggtaa aaaagcatgt
     4921 cctagcaaga cttctgacag acttctcagt gcacgatctg attttctgga gagatgtgaa
     4981 gaagactggg tttgtctttg gcaccacgct gatcatgctg ctttccctgg cagctttcag
     5041 tgtcatcagt gtggtttctt acctcatcct ggctcttctc tctgtcacca tcagcttcag
     5101 gatctacaag tccgtcatcc aagctgtaca gaagtcagaa gaaggccatc cattcaaagc
     5161 ctacctggac gtagacatta ctctgtcctc agaagctttc cataattaca tgaatgctgc
     5221 catggtgcac atcaacaggg ccctgaaact cattattcgt ctctttctgg tagaagatct
     5281 ggttgactcc ttgaagctgg ctgtcttcat gtggctgatg acctatgttg gtgctgtttt
     5341 taacggaatc acccttctaa ttcttgctga actgctcatt ttcagtgtcc cgattgtcta
     5401 tgagaagtac aagacccaga ttgatcacta tgttggcatc gcccgagatc agaccaagtc
     5461 aattgttgaa aagatccaag caaaactccc tggaatcgcc aaaaaaaagg cagaataaag
     5521 cggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc ttatcgataa
     5581 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5641 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5701 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5761 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5821 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5881 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5941 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6001 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6061 tccagcggac cttccttccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
     6121 acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct
     6181 gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact
     6241 tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt
     6301 cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata
     6361 aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct
     6421 tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc
     6481 tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg
     6541 tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat
     6601 ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga
     6661 aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc
     6721 gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag
     6781 tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt
     6841 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
     6901 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
     6961 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
     7021 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
     7081 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
     7141 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
     7201 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
     7261 gacgaagctt ggtaccgagc tcggatcaat tcgccaccat ggtgagcaag ggcgaggagc
     7321 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     7381 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     7441 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg
     7501 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     7561 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     7621 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     7681 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     7741 gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     7801 cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     7861 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     7921 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     7981 ccgggatcac tctcggcatg gacgagctgt acaagtaact cgagggaatt aattcgagct
     8041 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8101 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     8161 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8221 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8281 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8341 ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat
     8401 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa
     8461 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag
     8521 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat
     8581 ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt
     8641 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     8701 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     8761 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     8821 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     8881 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8941 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     9001 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     9061 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     9121 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     9181 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9241 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     9301 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9361 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9421 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9481 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9541 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9601 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9661 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9721 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9781 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9841 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9901 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9961 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    10021 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    10081 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    10141 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    10201 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    10261 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10321 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10381 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10441 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10501 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10561 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10621 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10681 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10741 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10801 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10861 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10921 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10981 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    11041 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    11101 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
    11161 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    11221 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    11281 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
    11341 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    11401 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    11461 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
    11521 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
    11581 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
    11641 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
    11701 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
    11761 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
    11821 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
    11881 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
    11941 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
    12001 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
    12061 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
    12121 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
    12181 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
    12241 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
    12301 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
    12361 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
    12421 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
    12481 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
    12541 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
    12601 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
    12661 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
    12721 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
    12781 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
    12841 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
    12901 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
    12961 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
    13021 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
    13081 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
    13141 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
    13201 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    13261 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    13321 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    13381 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    13441 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    13501 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
    13561 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
    13621 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
    13681 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
    13741 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
    13801 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
    13861 cggagtactt caagaactgc
//
