LOCUS       sequence-465717-        9230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing human SRF..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Christensen AH, Pan G, Marvig RL, Rodriguez Gonzalez FG, Vissing CR,
            Silajdzija E, Frosted R, Girma EG, Gabrielaite M, Jensen HK, Rossing
            K, Henriksen FL, Sandgaard NCF, Ahlberg G, Ghouse J, Lundegaard PR, 
            Weischenfeldt J, Wadelius C, Bundgaard H
  TITLE     Gain-of-function enhancer variant near KCNB1 causes familial 
            ST-depression syndrome.
  JOURNAL   Eur Heart J. 2025 Apr 10:ehaf213. doi: 10.1093/eurheartj/ehaf213.
  PUBMED    40208226
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur Heart 
            J. 2025 Apr 10:ehaf213. doi: 10.1093/eurheartj/ehaf213."
COMMENT     Sequence Label: pBMN-SRF
FEATURES             Location/Qualifiers
     source          1..9230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     194..211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        226..452
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             453..633
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1298..1531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1716..1760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2148..2527
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2528..2731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2682..2702
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2776..2795
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2776..2794
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4360..4386
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4396..4461
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4462..4515
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4531..4587
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4609..4674
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4687..5286
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4687..4706)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5183..5203
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5340..5360)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5758..5769)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5987..6201
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     6122..6141
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     LTR             6244..6424
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6496..6617
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6533..6552)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6587..6606
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6657..6792
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6825..6841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6825..6841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6838..6860)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6849..6865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6873..6879,6880..6897,6898..6903))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6918..6939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7056..7073)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7227..7815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7986..8777,8778..8846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8609..8628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8847..8951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9019..9037
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9075..9097)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9197..9216
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg acgcgtgtag tcttatgcaa
      241 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      301 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      361 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      421 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg tttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgggatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1621 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1681 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1741 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1801 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1861 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1921 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     1981 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcggac attgattatt
     2161 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     2221 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2281 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2341 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2401 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2461 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2521 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2581 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2641 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2701 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
     2761 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agcgtttaaa
     2821 cttaagcttg ccaccatgtt accgacccaa gctggggccg cggcggctct gggccggggc
     2881 tcggccctgg ggggcagcct gaaccggacc ccgacggggc ggccgggcgg cggcggcggg
     2941 acacgcgggg ctaacggggg ccgggtcccc gggaatggcg cggggctcgg gcccggccgc
     3001 ctggagcggg aggctgcggc agcggcggca accaccccgg cgcccaccgc gggggccctc
     3061 tacagcggca gcgagggcga ctcggagtcg ggcgaggagg aggagctggg cgccgagcgg
     3121 cgcggcctga agcggagcct gagcgagatg gagatcggta tggtggtcgg tgggcccgag
     3181 gcgtcggcag cggccaccgg gggctacggg ccggtgagcg gcgcggtgag cggggccaag
     3241 ccgggtaaga agacccgggg ccgcgtgaag atcaagatgg agttcatcga caacaagctg
     3301 cggcgctaca cgaccttcag caagaggaag acgggcatca tgaagaaggc ctatgagctg
     3361 tccacgctga cagggacaca ggtgctgttg ctggtggcca gtgagacagg ccatgtgtat
     3421 acctttgcca cccgaaaact gcagcccatg atcaccagtg agaccggcaa ggcactgatt
     3481 cagacctgcc tcaactcgcc agactctcca ccccgttcag accccacaac agaccagaga
     3541 atgagtgcca ctggctttga agagacagat ctcacctacc aggtgtcgga gtctgacagc
     3601 agtggggaga ccaaggacac actgaagccg gcgttcacag tcaccaacct gccgggtaca
     3661 acctccacca tccaaacagc acctagcacc tctaccacca tgcaagtcag cagcggcccc
     3721 tcctttccca tcaccaacta cctggcacca gtgtctgcta gtgtcagccc cagtgctgtc
     3781 agcagtgcca atgggactgt gctgaagagt acaggcagcg gccctgtctc ctctgggggc
     3841 cttatgcagc tgcctaccag cttcaccctc atgcctggtg gggcagtggc ccagcaggtc
     3901 ccagtgcagg ccattcaagt gcaccaggcc ccacagcaag cgtctccctc ccgtgacagc
     3961 agcacagacc tcacgcagac ctcctccagc gggacagtga cgctgcccgc caccatcatg
     4021 acgtcatccg tgcccacaac tgtgggtggc cacatgatgt accctagccc gcatgcggtg
     4081 atgtatgccc ccacctcggg cctgggtgat ggcagcctca ccgtgctgaa tgccttctcc
     4141 caggcaccat ccaccatgca ggtgtcacac agccaggtcc aggagccagg tggcgtcccc
     4201 caggtgttcc tgacagcatc atctgggaca gtgcagatcc ctgtttcagc agttcagctc
     4261 caccagatgg ctgtgatagg gcagcaggcc gggagcagca gcaacctcac cgagctacag
     4321 gtggtgaacc tggacaccgc ccacagcacc aagagtgaat acccatacga cgtgcctgac
     4381 tacgccgtca agctcgacta caaagaccat gacggtgatt ataaagatca tgacatcgat
     4441 tacaaggatg acgatgacaa ggagggcaga ggaagtctgc taacatgcgg tgacgtcgag
     4501 gagaatcctg ggcccgctag cggatcaggt gccacgaact tctctctgtt aaagcaagca
     4561 ggagacgtgg aagaaaaccc cggtccctta attaatggct ccggaccggt gaaacagact
     4621 ttgaattttg accttctcaa gttggcggga gacgtggagt ccaacccagg cccagtcgac
     4681 cccgggatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     4741 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     4801 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     4861 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     4921 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     4981 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     5041 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     5101 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     5161 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     5221 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     5281 gcctgatatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5341 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5401 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5461 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5521 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5581 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5641 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     5701 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5761 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5821 cgtctccgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     5881 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5941 ggggggactg gaagggctaa ttcactccca acgatgtcaa gaattggaac gctgacgtca
     6001 tcaacccgct ccaaggaatc gcgggcccag tgtcactagg cgggaacacc cagcgcgcgt
     6061 gcgccctggc aggaagatgg ctgtgaggga caggggagtg gcgccctgca atatttgcat
     6121 gtcgctatgt gttctgggaa atcaccataa acgtgaaatg tctttggatt tgggaatctt
     6181 ataagttctg tatgagacca cttggatcct ctgaattctt cgattctgct ttttgcttct
     6241 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6301 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6361 ttgtgtgact ctggtaacta gagatccctc agaccctttc agtcagtgtg gaaaatctct
     6421 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6481 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6541 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6601 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     6661 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6721 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     6781 tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata gctgtttcct
     6841 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6901 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6961 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7021 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7081 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7141 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7201 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7261 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7321 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7381 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7441 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7501 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7561 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7621 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7681 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7741 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7801 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7861 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7921 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7981 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8041 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8101 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8161 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8221 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8281 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8341 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8401 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8461 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8521 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8581 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8641 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8701 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8761 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8821 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8881 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8941 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9001 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     9061 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     9121 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     9181 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
//
