LOCUS       sequence-465718-        9104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing mouse MEF2C..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Christensen AH, Pan G, Marvig RL, Rodriguez Gonzalez FG, Vissing CR,
            Silajdzija E, Frosted R, Girma EG, Gabrielaite M, Jensen HK, Rossing
            K, Henriksen FL, Sandgaard NCF, Ahlberg G, Ghouse J, Lundegaard PR, 
            Weischenfeldt J, Wadelius C, Bundgaard H
  TITLE     Gain-of-function enhancer variant near KCNB1 causes familial 
            ST-depression syndrome.
  JOURNAL   Eur Heart J. 2025 Apr 10:ehaf213. doi: 10.1093/eurheartj/ehaf213.
  PUBMED    40208226
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur Heart 
            J. 2025 Apr 10:ehaf213. doi: 10.1093/eurheartj/ehaf213."
COMMENT     Sequence Label: pBMN-MEF2C
FEATURES             Location/Qualifiers
     source          1..9104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     194..211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        226..452
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             453..633
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1298..1531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1716..1760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2148..2527
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2528..2731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2682..2702
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2776..2795
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2776..2794
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4234..4260
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4270..4335
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4336..4389
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4405..4461
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4483..4548
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4561..5160
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4561..4580)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5057..5077
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5214..5234)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5632..5643)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5861..6075
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     5996..6015
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     LTR             6118..6298
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6370..6491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6407..6426)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6461..6480
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6531..6666
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6699..6715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6699..6715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6712..6734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6723..6739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6747..6753,6754..6771,6772..6777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6792..6813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6930..6947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7101..7689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7860..8651,8652..8720))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8483..8502
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8721..8825)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8893..8911
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8949..8971)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9071..9090
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg acgcgtgtag tcttatgcaa
      241 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      301 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      361 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      421 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg tttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgggatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1621 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1681 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1741 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1801 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1861 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1921 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     1981 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcggac attgattatt
     2161 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     2221 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2281 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2341 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2401 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2461 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2521 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2581 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2641 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2701 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
     2761 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agcgtttaaa
     2821 cttaagcttg ccaccatggg gagaaaaaag attcagatta cgaggataat ggatgagcgt
     2881 aacagacagg tgacttttac gaagaggaaa tttggattga tgaagaaggc ttatgagctg
     2941 agcgtgctgt gtgactgtga gattgcactg atcatcttca acagcaccaa caagctgttc
     3001 cagtacgcca gcactgacat ggataaggtg ttgctcaagt acaccgagta caacgagccg
     3061 cacgagagcc ggacaaactc agacattgtg gagacattga gaaagaaggg cctcaatggc
     3121 tgtgacagcc cagatcccga tgcagacgat tcagtaggtc acagccctga gtctgaggac
     3181 aagtacagga aaattaacga agatattgat ctaatgatca gcaggcaaag attgtgtgct
     3241 gttccacctc ccagctttga gatgccagtt accatcccag tgtccagcca taacagtttg
     3301 gtgtacagca atcctgtcag cacactggga aaccccaatc ttctgccact ggcccacccg
     3361 tctctgcaga ggaatagtat gtctcctggt gtaacacata gacctccaag tgcaggtaac
     3421 acaggcggtc tgatgggcgg agatctgaca tccggtgcag gcaccagcgc agggaatgga
     3481 tacggcaacc cccggaactc accaggcctg ctggtctcac ctggtaacct gaacaagaat
     3541 atacaagcca aatctcctcc ccctatgaat ctaggaatga ataatcgtaa gccagatctc
     3601 cgcgttctta tcccacctgg cagcaagaac acgatgccat cagtgaatca aaggataaat
     3661 aactcccagt cggctcagtc attggctacc ccggtggttt ccgtagcaac tcctacttta
     3721 ccaggacaag gaatgggagg atatccatca gccatttcaa caacatatgg tactgagtac
     3781 tctctgagta gcgcagatct gtcatctctg tctggcttca acactgccag tgcgctccac
     3841 ctcggctctg taactggctg gcagcagcag cacctacata acatgccgcc atctgccctc
     3901 agtcagttgg gagcttgcac tagcactcat ttatctcaga gttcaaatct ctccctgcct
     3961 tctactcaaa gcctcagcat caagtcagaa cctgtttctc ctcctagaga ccgtaccacc
     4021 accccttcga gatacccaca acacaccacg cgccacgagg cggggaggtc tcctgttgac
     4081 agcttgagca gctgtagcag ttcctacgat gggagcgacc gagaggatca ccggaacgaa
     4141 ttccactccc ccattggact caccagacct tcgccggacg aaagggaaag tccttcagtc
     4201 aagcgcatgc gactctctga aggatgggca acatacccat acgacgtgcc tgactacgcc
     4261 gtcaagctcg actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag
     4321 gatgacgatg acaaggaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
     4381 cctgggcccg ctagcggatc aggtgccacg aacttctctc tgttaaagca agcaggagac
     4441 gtggaagaaa accccggtcc cttaattaat ggctccggac cggtgaaaca gactttgaat
     4501 tttgaccttc tcaagttggc gggagacgtg gagtccaacc caggcccagt cgaccccggg
     4561 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     4621 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     4681 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4741 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     4801 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     4861 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     4921 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     4981 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5041 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     5101 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     5161 tatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5221 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5281 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5341 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5401 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5461 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5521 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     5581 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5641 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc
     5701 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt
     5761 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5821 actggaaggg ctaattcact cccaacgatg tcaagaattg gaacgctgac gtcatcaacc
     5881 cgctccaagg aatcgcgggc ccagtgtcac taggcgggaa cacccagcgc gcgtgcgccc
     5941 tggcaggaag atggctgtga gggacagggg agtggcgccc tgcaatattt gcatgtcgct
     6001 atgtgttctg ggaaatcacc ataaacgtga aatgtctttg gatttgggaa tcttataagt
     6061 tctgtatgag accacttgga tcctctgaat tcttcgattc tgctttttgc ttctactggg
     6121 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6181 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6241 gactctggta actagagatc cctcagaccc tttcagtcag tgtggaaaat ctctagcagt
     6301 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6361 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6481 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6541 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6601 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6661 gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt tcctgtgtga
     6721 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6781 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6841 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6901 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6961 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7021 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7081 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7141 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7201 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7261 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7321 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7381 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7441 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7501 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7561 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7621 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7681 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7741 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7801 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7861 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7921 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7981 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8041 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8101 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8161 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8221 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8281 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8341 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8401 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8461 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8521 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8581 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8641 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8701 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8761 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8821 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     8881 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     8941 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     9001 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     9061 cttaactatg cggcatcaga gcagattgta ctgagagtgc acca
//
