LOCUS       sequence-465723-        9248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing mouse MEF2D..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9248)
  AUTHORS   Christensen AH, Pan G, Marvig RL, Rodriguez Gonzalez FG, Vissing CR,
            Silajdzija E, Frosted R, Girma EG, Gabrielaite M, Jensen HK, Rossing
            K, Henriksen FL, Sandgaard NCF, Ahlberg G, Ghouse J, Lundegaard PR, 
            Weischenfeldt J, Wadelius C, Bundgaard H
  TITLE     Gain-of-function enhancer variant near KCNB1 causes familial 
            ST-depression syndrome.
  JOURNAL   Eur Heart J. 2025 Apr 10:ehaf213. doi: 10.1093/eurheartj/ehaf213.
  PUBMED    40208226
REFERENCE   2  (bases 1 to 9248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur Heart 
            J. 2025 Apr 10:ehaf213. doi: 10.1093/eurheartj/ehaf213."
COMMENT     Sequence Label: pBMN-MEF2D
FEATURES             Location/Qualifiers
     source          1..9248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     194..211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        226..452
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             453..633
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1298..1531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1716..1760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2148..2527
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2528..2731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2682..2702
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2776..2795
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2776..2794
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4378..4404
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4414..4479
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4480..4533
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4549..4605
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4627..4692
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4705..5304
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4705..4724)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5201..5221
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5358..5378)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5776..5787)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6005..6219
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     6140..6159
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     LTR             6262..6442
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6514..6635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6551..6570)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6605..6624
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6675..6810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6843..6859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6843..6859)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6856..6878)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6867..6883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6891..6897,6898..6915,6916..6921))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6936..6957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7074..7091)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7245..7833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8004..8795,8796..8864))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8627..8646
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8865..8969)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9037..9055
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9093..9115)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9215..9234
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg acgcgtgtag tcttatgcaa
      241 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      301 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      361 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      421 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg tttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgggatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1621 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1681 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1741 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1801 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1861 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1921 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     1981 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcggac attgattatt
     2161 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     2221 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2281 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2341 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2401 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2461 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2521 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2581 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2641 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2701 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
     2761 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agcgtttaaa
     2821 cttaagcttg ccaccatggg gaggaaaaag attcagatcc agcgaatcac tgatgaacgg
     2881 aaccgccagg tgaccttcac caagcggaag tttggactga tgaagaaggc ctacgagctg
     2941 agtgtgctgt gcgactgcga gatcgcgctc atcatcttca accactccaa caagctgttc
     3001 cagtatgcca gcaccgacat ggacaaggtg ctgctcaagt acaccgagta caacgagcca
     3061 cacgagagcc gcaccaatgc tgacatcatc gagaccctga ggaagaaggg tttcaacggc
     3121 tgtgacagcc cagagccgga tggggaggac tcactggagc agagccccct gctggaggac
     3181 aagtaccggc gggccagtga ggagctggat gggctcttca ggcgctatgg gtcatctgtt
     3241 ccggccccca actttgccat gcctgtcaca gtgcccgtgt ccaatcagag ctccatgcag
     3301 ttcagcaatc caagtagctc tctggtcact ccttccctgg tgacatcatc ccttacggac
     3361 ccacggctcc tgtcccccca gcagccagca ctacagagaa acagtgtttc tccaggcttg
     3421 ccccagcggc ctgctagtgc aggagccatg ctgggtggag acctcaacag tgctaatgga
     3481 gcctgcccca gccccgttgg gaatggctat gtcagtgccc gagcttcccc tggcctcctc
     3541 cctgtggcca atggcaacag cctaaacaaa gtcatccctg ccaagtctcc gcccccaccc
     3601 acccacaaca cccagcttgg agcccccagc cgcaagcctg atctgcgggt catcacttcc
     3661 cagggaggca aagggttaat gcatcatttg actggggacc atttagatct gaacaatgcc
     3721 cagcgccttg gggtctccca gtctacccac tcgctcacca ccccagtggt ttccgtggca
     3781 acaccaagtt tactcagcca gggcctcccc ttctcctcca tgcccactgc ctacaacaca
     3841 gattaccagc tgcccagtgc agagctatcc tccttaccag ccttcagttc acctgcaggg
     3901 ctggcactag gcaatgtcac cgcctggcag cagccccagc cgccccagca gccacaaccg
     3961 ccacaaccgc cacagtcaca gccacagcca ccacagccac agccacagca gccacctcag
     4021 caacagcccc acttggtccc cgtttctctc agcaacctca tccctggcag ccccttgcct
     4081 cacgtgggtg ctgctctcac agtcactacc cacccccaca tcagcatcaa gtcagaacca
     4141 gtgtccccaa gtcgtgaacg cagccctgca cctcctccac cagctgtgtt cccagctgcc
     4201 cgccctgagc ctggcgaagg tctcagcagc ccagctggag gatcctatga gaccggggac
     4261 cgggatgatg gacgggggga ctttgggccc acactaggcc tgctgcgccc agccccagag
     4321 cctgaggctg agggctcagc tgtgaagagg atgcggctgg atacttggac attaaagtac
     4381 ccatacgacg tgcctgacta cgccgtcaag ctcgactaca aagaccatga cggtgattat
     4441 aaagatcatg acatcgatta caaggatgac gatgacaagg agggcagagg aagtctgcta
     4501 acatgcggtg acgtcgagga gaatcctggg cccgctagcg gatcaggtgc cacgaacttc
     4561 tctctgttaa agcaagcagg agacgtggaa gaaaaccccg gtcccttaat taatggctcc
     4621 ggaccggtga aacagacttt gaattttgac cttctcaagt tggcgggaga cgtggagtcc
     4681 aacccaggcc cagtcgaccc cgggatgacc gagtacaagc ccacggtgcg cctcgccacc
     4741 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4801 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4861 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4921 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4981 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     5041 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     5101 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5161 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5221 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5281 atgacccgca agcccggtgc ctgatatcaa cctctggatt acaaaatttg tgaaagattg
     5341 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5401 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5461 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5521 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5581 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5641 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5701 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5761 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5821 gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg
     5881 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     5941 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gatgtcaaga
     6001 attggaacgc tgacgtcatc aacccgctcc aaggaatcgc gggcccagtg tcactaggcg
     6061 ggaacaccca gcgcgcgtgc gccctggcag gaagatggct gtgagggaca ggggagtggc
     6121 gccctgcaat atttgcatgt cgctatgtgt tctgggaaat caccataaac gtgaaatgtc
     6181 tttggatttg ggaatcttat aagttctgta tgagaccact tggatcctct gaattcttcg
     6241 attctgcttt ttgcttctac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6301 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6361 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag accctttcag
     6421 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6481 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     6541 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6601 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     6661 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6721 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6781 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa attcgtaatc
     6841 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6901 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6961 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7021 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7081 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7141 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7201 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7261 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7321 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7381 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7441 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7501 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7561 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7621 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7681 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7741 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7921 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7981 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8041 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8101 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8161 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8221 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8281 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8341 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8401 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8461 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8521 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8581 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8641 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8701 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8761 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8821 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8881 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8941 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     9001 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     9061 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     9121 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     9181 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     9241 gtgcacca
//
