LOCUS       sequence-465767-         437 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use as a MRE/CRE/SRE reporter for luciferase assays to measure GPCR 
            activation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Jiang Y, Xun Y, Zhang Z
  TITLE     Central regulation of feeding and body weight by ciliary GPR75.
  JOURNAL   J Clin Invest. 2024 Aug 13;134(19):e182121. doi: 10.1172/JCI182121.
  PUBMED    39137039
REFERENCE   2  (bases 1 to 437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2024-08-13"; volume: "134(19):e182121. doi"; pages:
            " 10.1172/JCI182121"
COMMENT     Sequence Label: pGL3-MRE/CRE/SRE
FEATURES             Location/Qualifiers
     source          1..437
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(211..222)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 ttacacagga tgtccatatt aggacatctg cgtcagcagg tttccacggc cacgcgtatg
       61 ctaaaggacg tcacattgca caatcttaaa tgctaaagga cgtcacattg cacaatctta
      121 aatgctaaag gacgtcacat tgcacaatct taactcgaga cttcaagccc tattcatccc
      181 atggccgtca tactgtgacg tctttcagag cactttgtga ttgctcagtc ctaagtataa
      241 gccctataaa atgatgggct ttgaaatgct ggtcagggta gagtgagaag caccagcagg
      301 cagtaacagc caacccttag ccattgctaa gggcagagaa ctggtggagc ctttctctta
      361 ctcccaggac ttcagcacct aagacagctc caaaacaaac cagaacagtc agctccgggg
      421 gagcacgact gggcgag
//
