LOCUS       sequence-465770-       16263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMOBC360_VM_C1 assembled plasmid (Chloramphenicol R) containing four
            inserts (POC1546-POC1549) assembled in POC1525 using the 
            site-selective methylation protection approach.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16263)
  AUTHORS   Flores-Fernandez CN, Lin D, Robins K, O'Callaghan CA
  TITLE     UniClo: scarless hierarchical DNA assembly without sequence 
            constraint.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf548. doi: 
            10.1093/nar/gkaf548.
  PUBMED    40548934
REFERENCE   2  (bases 1 to 16263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf548. doi"; 
            pages: " 10.1093/nar/gkaf548"
COMMENT     Sequence Label: POC1550
FEATURES             Location/Qualifiers
     source          1..16263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1909..2011
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2012..2671
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2078..2097)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3486..3705
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             3796..4551
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    4554..4804
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             5130..6305
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             6305..7276
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    7349..7822
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 gccctaaaaa gtaagtgagt gaccttataa gcaagaagga atgatagctt gaaacaatag
       61 ccaaggaagt tagagtcaca agatgtttca ttctctatag aaactaaaaa taacatctta
      121 acatatgtcc ctgagttgtt tttcagaaac atggattccc tcctaataga tccactgaca
      181 gggagacctt ataaagggga aactgagaac tgaactctga ccgccgttct ttgttctaaa
      241 tttcttcctg agggacctgg agagagtcaa gcccacaggc caggcctgaa cattcctctc
      301 ctttgcttcc ttatctatca caaatcagag aatctttaaa tctacctaag acctgtaagc
      361 tccaaaaaaa agccgcaaag gatacaaata agaagtttac aaaagatata gatttaaaaa
      421 aaaaaacaga aattctggaa ttaaagaatt catgacatga aattccaaat acatttgaaa
      481 gcttcaacaa catactagac acagctaatt tttttttttt tttttgtatt tttagtagag
      541 acgggatttc accaagttgg ccaggatggt ctcaatctct tgacatcatg atccacccac
      601 ctcagcctcc caaagtgctg ggattacagg tgtgagccac tgtgcccagc tctaacagca
      661 gatttcttag cagaaacctg aaggaaaaga actgccaagc caagagtcta caggaaatgc
      721 tcaagaaagt cctaaacctg gaattgaaac gacattttca acatttccac attatcacca
      781 tgaaaacata cacaagtata aaactcactg gtaaagcaat cacacaaaag aggaagagaa
      841 aggtctcaaa tggtcctact acagaaatcc accaaaccat aatgacacct ggcccaccca
      901 gggcggaaaa ctgcttaaag tcgttcttaa accacaaaca atagcatgag tgatctgtgc
      961 cttaaggaca tgctcctgct gcagataact agccaaaccc atccctttat tttggcccat
     1021 ccgtttgttt cccataagga atacttttag ttaatctata atctatagaa acaatgctta
     1081 tcactggctt gagttaataa atatgggggt aaatctctgt tcaaggctgt cagctctgaa
     1141 ggctgtgaga tccctgattt cccacttcac acctctatat ttctgtgtgt gtgtctttga
     1201 ttcctctagt gccactgggt tagggtcttc ccaactgagc tagtctcggc acaaagcctc
     1261 acatattaat aaaaatctgg aatgtaaaca gcaccacaat ccatagagac agaaggtaaa
     1321 agaatggcaa gaggttatca tcctcctggt caaaacgtcc ctgcagccaa acagaaattt
     1381 cctactcaag attcccagtg ggataataac aacatagctc ataggaggaa tatgagagac
     1441 ctgcaagaaa taataataaa aggaattaga gaatcgttac cctgcaccca gaatatgtgg
     1501 aattttttaa caggcttaag gaacaaatga ggaaatatgc tggtttagat ggaggagacc
     1561 cacttggaca aggaatgtta aagcttcact tcgtcacaaa tagctggcca gatgttactg
     1621 attcaagaat tttaaagtat gaagccatcc ccagagcaca gatgtttaga tttaattagt
     1681 tatcaaacag aagttagacc tgttttaaat gaaacaccct ttcagactgg gaaacacctg
     1741 tttatagatc aagtcgtgag aggtactgca ccacgaagtg tgcggccgcc ttcagcggcc
     1801 gcacgaataa atacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc
     1861 tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt
     1921 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
     1981 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     2041 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     2101 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     2161 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     2221 tccggagttc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     2281 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     2341 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     2401 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     2461 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     2521 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     2581 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     2641 gtactgcgat gagtggcagg gcggggcgta agagctggag cggcgtaacc gtcgcacagg
     2701 aaggacagag aaagcgcgga tctgggaagt gacggacaga acggtcagga cctggattgg
     2761 ggaggcggtt gccgccgctg ctgctgacgg tgtgacgttc tctgttccgg tcacaccaca
     2821 tacgttccgc cattcctatg cgatgcacat gctgtatgcc ggtataccgc tgaaagttct
     2881 gcaaagcctg atgggacata agtccatcag ttcaacggaa gtctacacga aggtttttgc
     2941 gctggatgtg gctgcccggc tccgggtgca gtttgcgatg ccggagtctg atgcggttgc
     3001 gatgctgaaa caattatcct gagaataaat gccttggcct ttatatggaa atgtggaact
     3061 gagtggatat gctgtttttg tctgttaaac agagaagctg gctgttatcc actgagaagc
     3121 gaacgaaaca gtcgggaaaa tctcccatta tcgtagagat ccgcattatt aatctcagga
     3181 gcctgtgtag cgtttatagg aagtagtgtt ctgtcatgat gcctgcaagc ggtaacgaaa
     3241 acgatttgaa tatgccttca ggaacaatag aaatcttcgt gcggtgttac gttgaagtgg
     3301 agcggattat gtcagcaatg gacagaacaa cctaatgaac acagaaccat gatgtggtct
     3361 gtccttttac agccagtagt gctcgccgca gtcgagcgac agggcgaagc cctcgagtga
     3421 gcgaggaagc accagggaac agcacttata tattctgctt acacacgatg cctgaaaaaa
     3481 cttcccttgg ggttatccac ttatccacgg ggatattttt ataattattt tttttatagt
     3541 ttttagatct tcttttttag agcgccttgt aggcctttat ccatgctggt tctagagaag
     3601 gtgttgtgac aaattgccct ttcagtgtga caaatcaccc tcaaatgaca gtcctgtctg
     3661 tgacaaattg cccttaaccc tgtgacaaat tgccctcaga agaagctgtt ttttcacaaa
     3721 gttatccctg cttattgact cttttttatt tagtgtgaca atctaaaaac ttgtcacact
     3781 tcacatggat ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg taaaaatagc
     3841 ccgcgaatcg tccagtcaaa cgacctcact gaggcggcat atagtctctc ccgggatcaa
     3901 aaacgtatgc tgtatctgtt cgttgaccag atcagaaaat ctgatggcac cctacaggaa
     3961 catgacggta tctgcgagat ccatgttgct aaatatgctg aaatattcgg attgacctct
     4021 gcggaagcca gtaaggatat acggcaggca ttgaagagtt tcgcggggaa ggaagtggtt
     4081 ttttatcgcc ctgaagagga tgccggcgat gaaaaaggct atgaatcttt tccttggttt
     4141 atcaaacgtg cgcacagtcc atccagaggg ctttacagtg tacatatcaa cccatatctc
     4201 attcccttct ttatcgggtt acagaaccgg tttacgcagt ttcggcttag tgaaacaaaa
     4261 gaaatcacca atccgtatgc catgcgttta tacgaatccc tgtgtcagta tcgtaagccg
     4321 gatggctcag gcatcgtgtc tctgaaaatc gactggatca tagagcgtta ccagctgcct
     4381 caaagttacc agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg tgttaatgag
     4441 atcaacagca gaactccaat gcgcctctca tacattgaga aaaagaaagg ccgccagacg
     4501 actcatatcg tattttcctt ccgcgatatc acttccatga cgacaggata gtctgagggt
     4561 tatctgtcac agatttgagg gtggttcgtc acatttgttc tgacctactg agggtaattt
     4621 gtcacagttt tgctgtttcc ttcagcctgc atggattttc tcatactttt tgaactgtaa
     4681 tttttaagga agccaaattt gagggcagtt tgtcacagtt gatttccttc tctttccctt
     4741 cgtcatgtga cctgatatcg ggggttagtt cgtcatcatt gatgagggtt gattatcaca
     4801 gtttattact ctgaattggc tatccgcgtg tgtacctcta cctggagttt ttcccacggt
     4861 ggatatttct tcttgcgctg agcgtaagag ctatctgaca gaacagttct tctttgcttc
     4921 ctcgccagtt cgctcgctat gctcggttac acggctgcgg cgagcgctag tgataataag
     4981 tgactgaggt atgtggtgtt cttatctcct tttgtagtgt tgctcttatt ttaaacaact
     5041 ttgcggtttt ttgatgactt tgcgattttg ttgttgcttt gcagtaaatt gcaagattta
     5101 ataaaaaaac gcaaagcaat gattaaagga tgttcagaat gaaactcatg gaaacactta
     5161 accagtgcat aaacgctggt catgaaatga cgaaggctat cgccattgca cagtttaatg
     5221 atgacagccc ggaagcgagg aaaataaccc ggcgctggag aataggtgaa gcagcggatt
     5281 tagttggggt ttcttctcag gctatcagag atgccgagaa agcagggcga ctaccgcacc
     5341 cggatatgga aattcgagga cgggttgagc aacgtgttgg ttatacaatt gaacaaatta
     5401 atcatatgcg tgatgtgttt ggtacgcgat tgcgacgtgc tgaggacgta tttccaccgg
     5461 tgatcggggt tgctgcccat aaaggtggcg tttacaaaac ctcagtttct gttcatcttg
     5521 ctcaggatct ggctctgaag gggctacgtg ttttgctcgt ggaaggtaac gacccccagg
     5581 gaacagcctc aatgtatcac ggatgggtac cagatcttca tattcatgca gaagatactc
     5641 tcctgccttt ctatcttggg gaaaaggacg atgtcactta tgcaataaag cccacttgct
     5701 ggccggggct tgacattatt ccttcctgtc tggctctgca ccgtattgaa actgagttaa
     5761 tgggcaaatt tgatgaaggt aaactgccca ccgatccaca cctgatgctc cgactggcca
     5821 ttgaaactgt tgctcatgac tatgatgtca tagttattga cagcgcgcct aacctgggta
     5881 tcggcacgat taatgtcgta tgtgctgctg atgtgctgat tgttcccacg cctgctgagt
     5941 tgtttgacta cacctccgca ctgcagtttt tcgatatgct tcgtgatctg ctcaagaacg
     6001 ttgatcttaa agggttcgag cctgatgtac gtattttgct taccaaatac agcaatagta
     6061 atggctctca gtccccgtgg atggaggagc aaattcggga tgcctgggga agcatggttc
     6121 taaaaaatgt tgtacgtgaa acggatgaag ttggtaaagg tcagatccgg atgagaactg
     6181 tttttgaaca ggccattgat caacgcagtt caactggtgc ctggagaaat gctctttcta
     6241 tttgggaacc tgtctgcaat gaaattttcg atcgtctgat taaaccacgc tgggagatta
     6301 gataatgaag cgtgcgcctg ttattccaaa acatacgctc aatactcaac cggttgaaga
     6361 tacttcgtta tcgacaccag ctgccccgat ggtggattcg ttaattgcgc gcgtaggagt
     6421 aatggctcgc ggtaatgcca ttactttgcc tgtatgtggt cgggatgtga agtttactct
     6481 tgaagtgctc cggggtgata gtgttgagaa aacctctcgg gtatggtcag gtaatgaacg
     6541 tgaccaggag ctgcttactg aggacgcact ggatgatctc atcccttctt ttctactgac
     6601 tggtcaacag acaccggcgt tcggtcgaag agtatctggt gtcatagaaa ttgccgatgg
     6661 gagtcgccgt cgtaaagctg ctgcacttac cgaaagtgat tatcgtgttc tggttggcga
     6721 gctggatgat gagcagatgg ctgcattatc cagattgggt aacgattatc gcccaacaag
     6781 tgcttatgaa cgtggtcagc gttatgcaag ccgattgcag aatgaatttg ctggaaatat
     6841 ttctgcgctg gctgatgcgg aaaatatttc acgtaagatt attacccgct gtatcaacac
     6901 cgccaaattg cctaaatcag ttgttgctct tttttctcac cccggtgaac tatctgcccg
     6961 gtcaggtgat gcacttcaaa aagcctttac agataaagag gaattactta agcagcaggc
     7021 atctaacctt catgagcaga aaaaagctgg ggtgatattt gaagctgaag aagttatcac
     7081 tcttttaact tctgtgctta aaacgtcatc tgcatcaaga actagtttaa gctcacgaca
     7141 tcagtttgct cctggagcga cagtattgta taagggcgat aaaatggtgc ttaacctgga
     7201 caggtcacgt gttccaactg agtgtataga gaaaattgag gccattctta aggaacttga
     7261 aaagccagca ccctgatgcg accacgtttt agtctacgtt tatctgtctt tacttaatgt
     7321 cctttgttac aggccagaaa gcataactgg cctgaatatt ctctctgggc ccactgttcc
     7381 acttgtatcg tcggtctgat aatcagactg ggaccacggt cccactcgta tcgtcggtct
     7441 gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac
     7501 cacggtccca ctcgtatcgt cggtctgata atcagactgg gaccacggtc ccactcgtat
     7561 cgtcggtctg attattagtc tgggaccatg gtcccactcg tatcgtcggt ctgattatta
     7621 gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctgga accacggtcc
     7681 cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc
     7741 tgattattag tctgggacca cgatcccact cgtgttgtcg gtctgattat cggtctggga
     7801 ccacggtccc acttgtattg tcgatcagac tatcagcgtg agactacgat tccatcaatg
     7861 cctgtcaagg gcaagtattg acatgtcgtc gtaacctgta gaacggagta acctcggtgt
     7921 gcggttgtat gcctgctgtg gattgctgct gtgtcctgct tatccacaac attttgcgca
     7981 cggttatgtg gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa ccgggctgca
     8041 tccgatgcaa gtgtgtcgct gtcgacgcaa gcggccgcac actctgtggt gcagtacctc
     8101 tcacgacttg gtctcctttc cccaagcctg acttgaactg tgaggcaatc acctgaggtc
     8161 aggagttcga gaccagcctg accaatacgg agaaactgtc tctactaaaa atacgggccc
     8221 agccagggtc cctcgtcctt ctcttgcttc caggcaggtc ctgcatccac tcctgctgca
     8281 agaagggctc ccatccctgc cttgggtctt tttcacaggt gtcaccctta cactctctgc
     8341 catgaccacc ttacgtgggt ggggctgagg ttgccttgac caagaagtgc atcagtttaa
     8401 aatgtaataa aataggccgg gtatggtggc tcatgcttgt aatcccagca ctttgggagg
     8461 ctgaggcagg cagatcacct gaggtcagga gtttgagacc agcctggcca acatggcaaa
     8521 atcctgtctc tatgaaaaat gcaaaaatta gccgggtatg gtggtgcatg tgcctcagct
     8581 cctgagtagc tgggactaca ggcacacgcc accaggccca gctaattttt tgtattttta
     8641 gtagagacag ggtttcacca tgttgatcag gatggtctcg atctcttgac ctgattttct
     8701 ccatgtggag taggggagac cccttagcct gaggctgcct ctgcccaccc tctgcacctg
     8761 ggaactgcca ctgccacagc caccatctcc acacagaacc tcctggagag gaaagggaaa
     8821 ttggctcagg gagaaagagt agggcctcga atggtgcaga ggacacgaca accccaagaa
     8881 agctcctgga ggaggtgcat gtgcaaagtg gacagagatg gccatgtgtg ctcaggaccc
     8941 tccctgttac aaggggtctc caaagggcta cacaggcagg gcccccaggc tgggcctcac
     9001 agctgccttc atccaggccc ttagcattca gcacgtgatt cccagctcac cccacctgga
     9061 tgcgccctag tgagttcaag agacacagag accgggatgg ggacagagca ggcgccgcag
     9121 ccctccctgc tgcccactcg cctccctgtg tgggctgagt gccggctcac ctccctgctg
     9181 agttccactg acccccattc ctcaccccca gcctgagcca gatccagtgg gaagagacag
     9241 gtccctgctc tctgccccta acttttctgg aaaaggcctc tccagtcact cttgcccctg
     9301 ccgactctca ccttctttct ggtccttgat atgagccagg gtcatcctga ggtcctttcc
     9361 gttccctgtc aagtccctgg tctctctgtc ccatgtctta tttcccagca cgggctctgg
     9421 ggaagaggaa atcacaggtg aaacttcttc ctggaagtaa cttcacattg atgtttaaca
     9481 cacaggtctg ctgtcccaac cttcctgggg caggaaatgc acacgggcaa agggacaaga
     9541 acgaggattt catatacaaa gaaaactgtg agggtgggag gatagaggag ggggctgatg
     9601 aacagaagaa gggggaatga agatggcaaa cttgtaggcc aggtgcaagg actgggtgtg
     9661 caggggcaag ggaggtaacc aaagtaaaat tgcataaatg ggatcatatc aagtaaaaag
     9721 gcttctgcac agcaaaggaa acaataaaca aaatgaagag accatccaag aatgggagaa
     9781 aatattttca aactattcat ctgacaaagg atcagtaact gaaacatata aggagctcga
     9841 acaagggcac tcgaatgcca ttcacaggag cagccccgga ggcagcccgg gaacccatgg
     9901 gcctcagaag gactggtttg tccgaaaagt gagaggaagc ggaggagacg cgaggagaga
     9961 aagagcagga ggagaccaga aagtgcaggt gatgcgcgat cccgaggagg actgaaaagt
    10021 gacggggaag cagggctgaa gtgtggcgga ggaccaatga aactggggcc cggaacttag
    10081 ggccaatcac gagcggagag ggcggggcca cgcttggaag agaaaattcc agtgggctgg
    10141 agacctgggg agatttagaa ggcgggacct ggggcccaga aagggagcgc agcctaaagc
    10201 agggaccagc ttcgagtagc tgagagtaca gctcattctg ctttcctgtc tgccaagcca
    10261 ttccaggcaa tcccttcatt tggtaaacat ttatcaaata cctactgtgt gcagggcatt
    10321 gttttaagag gagctgggac tgagataaga ggaaataaac cctccttgcc ctcaaggttt
    10381 atttcgataa aggttaaaaa attccattag tttttaaaaa gtttttcatt ttgaattttt
    10441 tgggggtttg tttggtctcc ttaggaatta tcttttattt cttgatatag ttcaaaattc
    10501 aaaattcaaa atgttcaaaa ggtaaaatgt ctctctctta ccctgtctca tccccctttt
    10561 cacacaaatt tcagggcacg gtgcatttat tgaatcagag acatcactaa gggatggaag
    10621 cactcagaca aggggacaaa caagagccca ttgagctaca cattacagtt gcccacagga
    10681 ctgtatgggc ctagagacaa gcagggaaga agagcactcc cctcctctcc aggcaggagt
    10741 gatggaccct catccccagg acgaggcagg gctgctgata cacagtaagg gcaggaggag
    10801 tttgtgtgga acccagagat tcacttgggg gcctccttgt ttcccttgtc ccattgtaaa
    10861 tgtgaacaga atcgtccagc aacccagtct gacagcgttt gatttccaaa ggcccagacc
    10921 cctcaggagg aagattggag ccgcatttcc aggaaatttc ccaaggcctc cctcccgtgc
    10981 tctgtcattc tcagcagcat tggtgcagag gccctgcttc ccatgggctg ttcccagcca
    11041 ctgacacgta acagcaggga cactaagaca gaccattcct gggcaacagg agactcctct
    11101 gttggccatc tgtggctgga ggactcctcc atggccttgc tcaactctcc tcagattgcc
    11161 tgtggtctag gatgcgtcca acaacccttc tttccttttg tccaggactg gagtcggcct
    11221 tgcctctgga tgtgccactt ttcccagggt tctctggctc cctccccata ttctctgaca
    11281 ggtgtgtccc ccaataaacc ttgtgagagg ccctgagcca gaagcactca aggaaatctc
    11341 tcccagattc ctgaccactg gaaactgtgg gagatgataa gtatttgttg ttttgggttg
    11401 tcaaacttca tgttatttat tatacaacaa tagacaatag cgcttcaaaa gagagaatga
    11461 attactacac tttaattttt atttattcta aattaactaa aagatattca gtgttatttg
    11521 gttttaagat ttcagtattt ccatgtatca ataggatgta ttttattaac attcacaaca
    11581 acctatttat ttgaacttca gtttcctacc acgacccaat taaacacaaa aggaagatcc
    11641 tggctatgcg aggatgattc agtctgcaaa ggcatctgaa tgtgtctgtg tccctgttag
    11701 cctagtgtga ggaggtggag agaccagccc tcccccatgt ctaccatgac ccccttcccc
    11761 atgctgaatt agctgacttt acaaaaagaa aattaccatc aataatgtgg atgggcctca
    11821 tccgatcagt agagtaagtg gttagacagg acaaggttga tttgagctaa tacttccggc
    11881 atgtcatatg gagttgccct gcacttctgg aaccgtctct cttggtcatg ggggctactg
    11941 tgacattgtg acttcggacc ccagagtgtt ctgagctctg agttgctctg ggctggcagt
    12001 gccctgtgcc agctgcactg aggcagccgt gttcctgctg gtcctacagg attccccacc
    12061 tgaaaccaga agcagatcag gatttcccaa attctctcta gccccgatgg gtgacatctc
    12121 cactgtttta aggaggcaat aattgcagct actacttctg tggtgggtgt tgccatgttc
    12181 cagacatctt tctgggtact tggcatatgt aatggcaaat tatcttaaaa ctggggaaaa
    12241 ctttttcatg ttcagatttt aaaaaaaacc tataatcact gacatgtcca atttctgatt
    12301 tttcaaataa gatagatttc ttgtctgcaa caataaaaca gcctcccaga aacatcactg
    12361 gatcttactc agtcacaaac caccctcccc aaccctagag tagccactcc tgatttctaa
    12421 cacagataag ttctgccagt tccagttgaa tggcattata cagtcaccat catgctcctg
    12481 ggtctcatca tcctgctgtg tgtggtcata gcctattcac tcccatggct gtccaatgct
    12541 ccactttata aatagctcac atttcttgtc cctttgctgt tgatagacaa ttgtgttata
    12601 tccagatttt acaaatcgtg acacgcaaat gttgcagcct tgtttgtaac agtgaaaaat
    12661 tgaaaacaac ctggaagtcc agtgatggga aaatgaatat atttctgtct tttggggaac
    12721 ccaaagcagg ttccaagact gcaatttcag tgaaagcagt cagtgaatgg caaacactta
    12781 aagcaatttc atgtttaagt atataagctc ttcatatctt tttttttttt ttgacagagt
    12841 ttcgctcttg ttaccctgac ggagtgcaat ggcacaactt cagctcactg caacctctga
    12901 cttcccaggt tcaaatgatt ctcctgcctc aggctcccat gtagctggga ctacaggcac
    12961 gtgccacgac gcctggctaa ttttgtgttt ttggtgaaga cggggtttca caatgttggt
    13021 caggctggtc tcaaactcct gacctaaagt gatccgcctg cctcggcctc ccaaagtgct
    13081 gggattatag gcttgtttga gatgcctttg agaaatgaag aaagtagaga gagaataaaa
    13141 gaaaaactgg ccaggcacag tggctcatgc ctgtaataca gtggctcagc attttgggag
    13201 gcagagacag gcagatacct tgagatcagc ttgggcaaca tggtgaagcc ccatctctac
    13261 aaaaaataca aaagttagct gggcattgtg gcgcacacct gtagtcccat ctactcaggg
    13321 agctgagatg gaaggatcaa ttgagcctgc gagttcgagg ctgcagtgag ttgtgattgt
    13381 gccactgcac tccagcctgg gtgacagaag agaccttgtc tcgaaaaaga agctgaaaac
    13441 aatggaacca tgccttcaga attccagaaa gaaagttatt ttcaacgaat aaatctatat
    13501 tcagccaaat aatcaagggt gaaggtaaaa taatacattt ttagagactc aggggttacc
    13561 tttattttta tatcttttag agacgaggtt ctcacaatgt tgttcaagct ggtctccaac
    13621 tcctgatctc aagcaatcct cttgccttag cgtcctgaat tgctgggatt acaggggtga
    13681 gctgtcatgc ccagcttgta atgtactgac accttcaaga tttccttctc caagtgtggc
    13741 atacatgacc ctttgccaat ttttgatagg gctgtttttt ttcttgtaaa tttgtttaag
    13801 ttccttgtaa acatacatgt gagctctcat cattcttgtt taaacaccta agaggcatcc
    13861 aaatcagtgc aacatggcaa gaaaatgaaa taagaaacca atagaaggac caggcatggt
    13921 ggctcatgcc tgtaatccct gcattttggg aggttgaggt ggaaggatca cttgagttca
    13981 ggagtttgag accagcctga tagtgagacc tcatctctac caaataaaaa taattttaaa
    14041 agaaaaaaga tcaatagata ggaaaggaag aaacaaaaga atggcatcat ctcacacatc
    14101 gttatgctaa aggagagacc aaacgaagga ctacataaca tacgactgca cgtccattac
    14161 attctagaaa tttcagtatt gcagtgacag aaggggagag gagggtgtga gggaagagga
    14221 cagagagaag ggctggggaa gcatacaaca ataatagcac aatggtaggt tacaagaaac
    14281 tacactgtta gaagtgtcct ttattttgct ggatgcagct taatataacc tgaacttcac
    14341 tgtgaaaagt caaggaatcg ggtttccatt ctttgctatt gtgtaaagaa atatagccaa
    14401 aagccactag gagaattaaa accataaact aaaaaatgtt tatttgacac ataagaaagt
    14461 agtaaaggag gaacagaaac aaaaagatat gagacaaatt gaaaacgtat agcaaaatgg
    14521 tagaccaaaa cccaaccatt gcaagtgaag aaatgacatt agtgtgctac ctactgtatt
    14581 aacaaacaaa aaggaaactg gtctctatga gaatccctat gcgtgaggag tatttaaatt
    14641 aggagggtcg ggcgggtctc agcccctcct cacccccagg ctcccactcc atgaggtatt
    14701 tctacacctc cgtgttatat aaatttaatt tatatttaaa tttaaagtat atatactagt
    14761 aaaatttata tacatatttt aagatatatc aaatattaat tccgcggggc cggggccagg
    14821 gtctcacacc ctccagagca tgtacggctg cgacgtagta ttaatatata tctagtaaat
    14881 tgggcatgac cagtacgcct acgacggcaa ggattacatc gccctgaacg aggacctgcg
    14941 ctcctggatc agagactcga actttccaat ccaaatggat ggggcctgtg cccatttggt
    15001 gttgagtcca tcatgggctt tatgtggtta gtcctcagct gggtcacctt cactgctcca
    15061 ttgtccttgt cccttcagtg gaaacttgtc cagtgggagc tgtgaccaca gaggctcaca
    15121 catcgcccag ggcggcccct gcacacgggg gtctctgtgc attctgagac aaattttcag
    15181 agccattcac ctcctgctct gcttctagag ctccttttct gctctgctct tctgccctct
    15241 ctccctgccc tggttctagt ggagctgctg tggctggaga aatagattta ggaggtcaca
    15301 ccatatatat gagacttaaa acctcaggca tggatgaagc accaagggag tgactgacta
    15361 tggaaaagaa tgagcacaag gactgaaccc tggaccagtt ctaaggggtg tgatcagacc
    15421 acacccagag cagactgcac agttctggtc ccacgtctag aggacactca gacaaggaac
    15481 ccccatattc cttaaatgta ccttgagcac atgttctcag ggtctcctga gggctgtgtc
    15541 atgggctgtg gtcactcatt gttggtctct gtcaggaagt tgctatagcc atgtaaacca
    15601 aaaaacgtct gagacaggtc tcagtcagtt tagaagttta tttcaccaag ctgaagatgc
    15661 accttggaaa cagagacaca aatcattgta gcatttgggg gaaacattca aaccatagca
    15721 gttataaagg atacaaatga gcagccagat gaagaggtgc acagtgaggc ctggaagggc
    15781 tctgagcaca agagcctctg tccccacgga gacaagtgca caccaacatg cccaactaat
    15841 ttttaaatat tttgtaggaa tgggtctcac tattctgccc agtctggtct caaactcctg
    15901 gacttaaaca atgttcctac cattgcctcc caaactgatg gaattataag tgtgagccac
    15961 tgtgcctggc caatcacaga tctcctgatc ctggcacatt accagaatcc tctttggtca
    16021 ttaacaatta tcccagtgca atccataaac aggaggatat gcaggtgcat tttctggtgt
    16081 aaagaaatat gactcggtgg tccacatggt tgcagtacct cccattcaag ttcttttgta
    16141 acactcgagc atttgcatct gagataaagc agggacctct tttagaagcc tgctggggcc
    16201 tcccaatcta gaaataaaga aaaaccttca gtcccttcaa gagaaattcc aggcacctag
    16261 cca
//
