LOCUS       sequence-465772-        5015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the expression of wrmScarlet1-10 and GFP1-10 in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Kurashina M, Snow AW, Mizumoto K
  TITLE     A modular system to label endogenous presynaptic proteins using 
            split fluorophores in C. elegans.
  JOURNAL   Genetics. 2024 Dec 22:iyae214. doi: 10.1093/genetics/iyae214.
  PUBMED    39708832
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2024 Dec 22:iyae214. doi: 10.1093/genetics/iyae214."
COMMENT     Sequence Label: pSM.wrmScarlet1-10::SL2::GFP1-10
FEATURES             Location/Qualifiers
     source          1..5015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             75..86
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2585..2607)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2707..2726
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2808..2826)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2894..2998
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2999..3067,3068..3859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3217..3236)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4030..4618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4519..4538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4772..4789
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4906..4927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4942..4947,4948..4965,4966..4972)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4980..4996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4985..5007
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(5004..5015,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(5004..5015,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccactgact
       61 gggcgcgcca tggtatcgaa gggagaagca gtaatcaagg agttcatgcg tttcaaggtc
      121 cacatggagg gatccatgaa cggacacgag ttcgagatcg agggagaggg agagggacgt
      181 ccatacgagg gaacccaaac cgccaagctc aaggtcacca aggtaagttt aaacatatat
      241 atactaacta accctgatta tttaaatttt cagggaggac cactcccatt ctcctgggac
      301 atcctctccc cacaattcat gtacggatcc cgtgccttca tcaagcaccc agccgacatc
      361 ccagactact acaagcaatc cttcccagag ggattcaagt gggagcgtgt catgaacttc
      421 gaggacggag gagccgtcac cgtcacccaa gacacctccc tcgaggacgg aaccctcatc
      481 tacaaggtaa gtttaaacag ttcggtacta actaaccata catatttaaa ttttcaggtc
      541 aagctccgtg gaaccaactt cccaccagac ggaccagtca tgcaaaagaa gaccatggga
      601 tgggaggcct ccaccgagcg tctctaccca gaggacggag tcctcaaggt aagtttaaac
      661 atgattttac taactaacta atctgattta aattttcagg gagacatcac catggccctc
      721 cgtctcaagg acggaggacg ttacctcgcc gacacctcca ccacctacaa ggccaagaag
      781 ccagtccaaa tgccaggagc ctacctcgtc gaccgtaagc tcgacatcac ctcccacaac
      841 gagtactaag gtaccgctgt ctcatcctac tttcacctag ttaactgctt gtcttaaaat
      901 ctatgcttct ctttagtatc taaaattttc ctagaagctt acaagtatat aaatggtctc
      961 ttctcaataa aggttgtata tttattcatc ttattgaatc tgccatttcc tcgtttttgc
     1021 gagtttatat accttccaat tttctttcta ttgtattttc aacttctaat tttaattcag
     1081 ggaaactgct gtaccgatat catgtcaaag ggagaagaac tcttcacagg tgtcgtgcca
     1141 attctcgttg aactcgacgg agatgttaac ggacacaagt tctcagttcg cggagaggga
     1201 gagggagatg ctacaattgg aaaactcacc ctcaagttca tctgcaccac tggaaaactt
     1261 ccagtgccat ggccaacact cgttacaaca ctcacatacg gtgtccagtg cttctcacga
     1321 tacccagatc atatgaagcg ccacgacttc ttcaaatcgg ctatgccaga aggatacgtc
     1381 caagagcgaa ccatctcgtt caaggatgac ggaaagtaca agacacgagc cgttgttaag
     1441 ttcgagggag atacactcgt gaaccgcatt gaactcaagg gaaccgattt caaagaggac
     1501 ggaaacatcc tcggacataa gctcgagtac aacttcaact cgcacaacgt gtacatcacc
     1561 gccgataagc aaaagaacgg aatcaaggcc aacttcaccg ttcgacacaa tgtcgaggat
     1621 ggatcggttc aacttgccga tcactaccaa caaaacaccc caattggaga tggaccagtg
     1681 ctccttccag ataaccatta cctctcgaca caaaccgtgc tctcgaagga tccaaatgag
     1741 aaaggaacag gtggtggtgg atcgtagagc tccgcatcgg ccgctgtcat cagatcgcca
     1801 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct
     1861 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
     1921 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga
     1981 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
     2041 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
     2101 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     2161 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg
     2221 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga
     2281 aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg
     2341 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa
     2401 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag
     2461 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa
     2521 ctgtttctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg
     2581 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     2641 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     2701 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     2761 acagatgcgt aaggagaaaa taccgcatca ggcggcctta attaagggcc tcgtgatacg
     2821 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2881 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2941 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3001 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3061 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3121 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3181 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3241 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3301 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3361 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3421 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3481 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3541 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3601 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3661 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3721 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3781 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3841 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3901 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3961 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4021 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4081 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4141 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4201 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4261 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4321 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4381 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4441 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4501 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4561 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4621 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4681 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4741 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4801 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     4861 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     4921 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     4981 tgtgagcgga taacaatttc acacaggaaa cagct
//
