LOCUS       sequence-465803-        6032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' AAVLINK plasmid for CECR2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Zhonghua Lu Lab
  TITLE     AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery 
            in Gene Therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-5' CECR2
FEATURES             Location/Qualifiers
     source          1..6032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        186..397
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             410..678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             715..741
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     repeat_region   3295..3435
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3295..3424
                     /label=AAV2 ITR
     rep_origin      3510..3965
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3597..3616)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3807..3828
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3982..4001)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4101..4123
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4161..4179)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4247..4351
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4352..4420,4421..5212)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4570..4589)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5383..5971
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5872..5891
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggatc tgcgatcgct ccggtgcccg
      181 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
      241 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
      301 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
      361 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
      421 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
      481 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
      541 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
      601 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
      661 tctgcgccgt tacagatcca agctgtgacc ggcgcctacc gtacggccac catgtaccct
      721 tatgacgtac ctgactatgc tggatcttgc ccagaggagg gcggcgcggc cgggctgggc
      781 gagctccgct cctggtggga ggtcccggcc atcgcgcact tctgctcgct ctttcgcacc
      841 gcgttccgcc tgcccgactt cgagatcgag gagttagaag ccgctcttca cagagatgac
      901 gtggagttta tcagtgacct gattgcctgc ctgcttcagg gctgctatca acgaagagat
      961 atcacgcctc agacattcca cagctaccta gaggacatca tcaactaccg ctgggagctc
     1021 gaagaaggga agcccaaccc tctgagggaa gccagtttcc aggacctgcc tcttcgcaca
     1081 cgggtggaga tcctgcaccg actctgtgat taccggctgg atgcagacga tgtcttcgat
     1141 cttctaaagg gcctggatgc agacagtctc cgtgtggagc cattgggtga agacaattct
     1201 ggggcactat attggtattt ctatggaaca cgaatgtaca aagaggaccc ggtgcaagga
     1261 aaatccaatg gagaactctc tttgagcagg gaaagtgaag gacaaaaaaa tgtctcaagt
     1321 attcctggaa aaacgggaaa aagaagagga agacccccaa aacggaagaa actgcaggag
     1381 gagattctgt tgagtgaaaa gcaggaagaa aattccttgg catccgagcc acagacaaga
     1441 catgggtccc aagggccagg ccaaggtact tggtggctcc tgtgccagac agaagaggaa
     1501 tggagacagg tcaccgagag ttttcgcgag aggacctccc ttcgagaacg gcagctctac
     1561 aagctcctca gtgaggactt cctgcctgag atctgcaaca tgatcgccca gaaggagact
     1621 cctgtgctga ccagaataga aaaacaaaag cgcaaagagg aggaagaaga gcgtcagatt
     1681 cttctagcag tgcagaagaa ggagcaggag cagatgctaa aggaagagag gaaacgcgag
     1741 ttggaggaga aggtcaaggc agtggaagat cgagcgaaga ggagaaagct cagggaagaa
     1801 agggcatggc tgctggctca aggaaaggag ctccctccag aactttccca tctggacccc
     1861 aattccccca tgagagagga aaaaaagact aaagacctct ttgagttgga tgatgatttc
     1921 actgctatgt ataaagttct agacgtggta aaggctcaca aggattcctg gcccttcttg
     1981 gaacctgtgg atgaatctta tgcccctaac tattatcaga ttattaaggc ccccatggat
     2041 atttccagca tggagaagaa actgaatgga ggtttatact gtaccaagga ggaatttgta
     2101 aatgacatga agaccatgtt caggaattgt cgaaagtata atggggaaag tagtgagtat
     2161 accaagatgt ctgataattt agagaggtgt ttccatcggg caatgatgaa acattttcct
     2221 ggagaagatg gagacacaga tgaagaattt tggattcgag aggatgaaaa gcgggagaaa
     2281 agacggagtc gggctgggcg aagtggtggg agccatgttt ggacccgctc cagggaccca
     2341 gaagggtcca gcaggaaaca gcagcccatg gagaatggag gaaagtcgtt gccccccaca
     2401 cgccgagcgc cctcttctgg ggacgatcag agcagcagct ccacacagcc cccgcgggag
     2461 gtgggcactt ccaatggccg aggtttttct catcccctgc attgtggtgg gacacccagc
     2521 caggcaccct ttttaaacca gatgaggcca gcagtaccag gaacatttgg ccctctgcga
     2581 ggatcagatc ctgccacctt gtatggctcc tctggagtcc cggagccaca ccacggggag
     2641 cctgtgcagc agcgtcagcc tttcaccatg cagcctccag ttggaattaa cagcctccga
     2701 ggacccaggc taggcacacc agaggagaag caaatgtgcg gggggctgac acacctttct
     2761 aacatgggcc cacaccctgg atccttgcag cttgggcaga taagtggccc aagtcaggat
     2821 ggaagcatgt atgctccagc tcagttccag ccaggattca ttcctccccg gcatgggggg
     2881 gctccagccc ggccaccaga ctttcctgaa agctcagaaa ttcctcccag ccatatgtat
     2941 cgatcgtaca agtacctgaa tcgagtacac tctgccgtct ggaatgggaa ccatggtgct
     3001 acgaaccaag gacccttggg cccagatgag aagccccacc tggggccagg accctctcac
     3061 cagcctcgca ctctcggtca cgtgatggat tcccgagtca tgagaccacc tgtccccccc
     3121 aaccagtgga ctgaacaatc aggcttccta cctcatggag ttccttcctc aggtaagtat
     3181 caaggttaca agacaggttt aaggagacca ataagctatt attcgtatag gatactttat
     3241 acgaagttat taagttatgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac
     3301 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     3361 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     3421 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3481 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     3541 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
     3601 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3661 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3721 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3781 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3841 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
     3901 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     3961 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     4021 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4081 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     4141 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4201 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4261 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4321 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4381 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4441 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4501 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4561 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4621 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4681 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4741 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4801 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4861 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4921 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4981 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5041 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5101 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5161 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5221 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5281 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5341 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5401 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5461 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5521 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5581 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5641 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5701 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5761 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5821 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5881 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5941 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6001 cctggccttt tgctggcctt ttgctcacat gt
//
