LOCUS       sequence-465911-        7931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Galactose iduced expression of Gcn4  E+GFP in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Bremer A, Lang WH, Kempen RP, Sweta K, Taylor AB, Borgia MB, Ansari 
            AZ, Mittag T
  TITLE     Reconciling competing models on the roles of condensates and soluble
            complexes in transcription factor function.
  JOURNAL   bioRxiv [Preprint]. 2024 Nov 22:2024.11.21.624739. doi: 
            10.1101/2024.11.21.624739.
  PUBMED    39605529
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Nov 22:2024.11.21.624739. doi: 
            10.1101/2024.11.21.624739."
COMMENT     Sequence Label: pAG415Gal_Gcn4 E3GFP
FEATURES             Location/Qualifiers
     source          1..7931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..27)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    69..572
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        609..713
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(714..782,783..1574)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(932..951)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1745..2333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2234..2253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2487..2504
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2621..2642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2657..2662,2663..2680,2681..2687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2695..2711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2700..2722
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2719..2735
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2719..2735
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2754..2774
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2756..2774
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2798..3239
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2798..2915
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     3210..3233
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     3254..3270
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3254..3270
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    3278..3302
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3305..3325
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3596..3652
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3689..3715
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3731..4441
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3758..3786)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4381..4401
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             4463..4489
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(4500..4524)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4558..4805
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4574..4592)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4823..4842)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4824..4842)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4852..4869)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4852..4868)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4861..4883)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5009..5464
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5096..5115)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5306..5327
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5765..6172
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6173..7267
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(7761..7780)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7880..7902
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
       61 ttaggacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
      121 tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta
      181 aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa
      241 tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag
      301 atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt
      361 ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa
      421 aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta
      481 ttttttcttt aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa
      541 tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg
      601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1861 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2521 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2581 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     2641 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     2701 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     2761 accctcacta aagggaacaa aagctggagc tctagtacgg attagaagcc gccgagcggg
     2821 tgacagccct ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc
     2881 tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag
     2941 cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatga
     3001 acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg
     3061 ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atgcaaaaac
     3121 tgcataacca ctttaactaa tactttcaac attttcggtt tgtattactt cttattcaaa
     3181 tgtaataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga
     3241 aaaaaccccg gattctagaa ctagtggatc ccccatcaca agtttgtaca aaaaagcagg
     3301 cttcgagaac ctgtattttc agggtcatat gagcaccgat agcgaaccga tgtttgaata
     3361 tgaaaacctg gaagataaca gcgaagaatg gaccagcctg tttgataatg atattccggt
     3421 taccaccgat gatgttagcc tggcagatga agcaattgaa agcggtagcg gttcaggtag
     3481 cggtagtccg gaatccagtg atcctgctgc tctaaaacgt gctagaaaca ctgaagccgc
     3541 caggcgttct cgtgcgagaa agttgcaaag aatgaaacaa cttgaagaca aggttgaaga
     3601 attgctttcg aaaaattatc acttggaaaa tgaggttgcc agattaaaga aattagttgg
     3661 cgaacgcacc ggtggtggat ccggtggtta cccatacgat gttcctgact atgcgggcta
     3721 tccctatgac agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     3781 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     3841 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     3901 aacacttgtc actactttca cttatggtgt tcaatgcttt tcaagatacc cagatcatat
     3961 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     4021 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     4081 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     4141 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
     4201 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     4261 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     4321 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     4381 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     4441 agtcccggac tatgcaggat cctatccgta tgacgttcca gattacgctg ctcagtaaga
     4501 cccagctttc ttgtacaaag tggtgatggg ctgcaggaat tcgatatcaa gctcgagtca
     4561 tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa
     4621 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag
     4681 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg
     4741 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa
     4801 tttgcggccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     4861 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     4921 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     4981 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     5041 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5101 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5161 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5221 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5281 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5341 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5401 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5461 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatcga
     5521 ccggtcgagg agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg
     5581 gaatcccaac aattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc
     5641 ttgttcatgt gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca
     5701 agtaattggt tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc
     5761 agttaactgt gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc
     5821 attatttttt tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt
     5881 cgatgactgg aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac
     5941 tgaaaaattg ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata
     6001 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
     6061 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
     6121 cttttacatt tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc
     6181 ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc
     6241 cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa
     6301 tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct
     6361 ggaagcctcc aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg
     6421 taccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta
     6481 cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa
     6541 gccacaattt gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta
     6601 ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac
     6661 cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc
     6721 accattgcct atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag
     6781 aaaaactgtg gaggaaacca tcaagaacga attccctaca ttgaaggttc aacatcaatt
     6841 gattgattct gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat
     6901 caccagcaac atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt
     6961 gggtttgttg ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt
     7021 gtacgaacca tgccacggtt ctgctccaga tttgccaaag aataaggtca accctatcgc
     7081 cactatcttg tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa
     7141 ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg
     7201 tggttccaac agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct
     7261 tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa atgaaattca
     7321 taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat
     7381 acgcaatcta catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta
     7441 agcaaattga tactaatggc tcaacgtgat aaggaaaaag aattgcactt taacattaat
     7501 attgacaagg aggagggcac cacacaaaaa gttaggtgta acagaaaatc atgaaactat
     7561 gattcctaat ttatatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa
     7621 aacactcaat gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc
     7681 cataatggtg aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag
     7741 tcgatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     7801 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     7861 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     7921 aaacgcgcga g
//
