LOCUS       sequence-465957-        8776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector for NADK (D184A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Flickinger KM, Mellado Fritz CA, Huggler KS, Wade GM, Chang GR, Fox 
            KC, Simcox JA, Cantor JR
  TITLE     Cytosolic NADK is conditionally essential for folate-dependent 
            nucleotide synthesis.
  JOURNAL   Nat Metab. 2025 May 2. doi: 10.1038/s42255-025-01272-3.
  PUBMED    40316835
REFERENCE   2  (bases 1 to 8776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2025 May 2. doi: 10.1038/s42255-025-01272-3."
COMMENT     Sequence Label: pLJC2-NADK (D184A)-3xFLAG
FEATURES             Location/Qualifiers
     source          1..8776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    380..505
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             552..732
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(733..959)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(987..1007)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(987..1005)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1026..1042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1026..1042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1039..1061)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1050..1066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1074..1080,1081..1098,1099..1104))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1119..1140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1257..1274)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1428..2016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1508..1527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2187..2978,2979..3047))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2810..2829
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3048..3152)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3178..3633)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3315..3336)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3527..3546
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3760..3782
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3774..3791
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3775..3791
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3801..3820
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3801..3819
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3840..3975
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4015..4136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4026..4045)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4080..4099
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4208..4441
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4569..5168)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4652..4672)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5149..5168
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5190..5700)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5253..5271)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    5749..5866
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5914..5979)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(7391..7594)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7420..7440)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(7883..7903)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(8201..8245)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8430..8663
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gtccctcata tctcctcctc caggtctgaa gatcagctgc tgcttgctgt gcggtggtct
       61 tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta
      121 tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg
      181 atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt
      241 ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt
      301 ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa
      361 ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag
      421 tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag
      481 caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt
      541 tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
      601 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
      661 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
      721 ccagagagac ccgtttatgt atcgagctag gcacttaaat acaatatctc tgcaatgcgg
      781 caattcagtg gttcgtccaa tccatgtcag acccgtctgt tgccttccta ataaggcacg
      841 atcgtaccac cttacttcca ccaatcggca tgcacggtgc tttttctctc cttgtaaggc
      901 atgttgctaa ctcatcgtta ccatgttgca agactacaag agtattgcat aagactacat
      961 taagcttgca gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     1021 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1081 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1141 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1201 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1261 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1321 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1381 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1441 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1501 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1561 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1621 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1681 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1741 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1801 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1861 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1921 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1981 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2041 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2101 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2161 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2221 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2281 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2341 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2401 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2461 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2521 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2581 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2641 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2701 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2761 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2821 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2881 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2941 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3001 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3061 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3121 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     3181 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     3241 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     3301 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     3361 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     3421 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     3481 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     3541 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     3601 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     3661 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     3721 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     3781 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt acgtccctag
     3841 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     3901 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     3961 agttaggggc gggatgggcg gagttagggg cgggatagct agagccagac atgataagat
     4021 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     4081 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttcctc
     4141 tcactctctg atattcattt ctttgcaagt tataaatact gaataataag atgacatgaa
     4201 ctactactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     4261 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
     4321 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     4381 agagacccag tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat tagcccttcc
     4441 agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt
     4501 cttaaaggta ccgatgcatg gggtcgtgcg ctcctttcgg tcgggcgctg cgggtcgtgg
     4561 ggcgggcgtc aggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc
     4621 tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg
     4681 gaggtctcca ggaaggcggg caccccggcg cgctcggccg cctccactcc ggggagcacg
     4741 acggcgctgc ccagaccctt gccctggtgg tcgggcgaga cgccgacggt ggccaggaac
     4801 cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc
     4861 agccgggaac cgctcaactc ggccatgcgc gggccgatct cggcgaacac cgcccccgct
     4921 tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc
     4981 ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt gcagctcggt gacccgctcg
     5041 atgtggcggt ccggatcgac ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg
     5101 agggtgcgta cggccctggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac
     5161 tcggtcatgg taagctccgg tggatccccc tggggagaga ggtcggtgat tcggtcaacg
     5221 agggagccga ctgccgacgt gcgctccgga ggcttgcaga atgcggaaca ccgcgcgggc
     5281 aggaacaggg cccacactac cgccccacac cccgcctccc gcaccgcccc ttcccggccg
     5341 ctgctctcgg cgcgccctgc tgagcagccg ctattggcca cagcccatcg cggtcggcgc
     5401 gctgccattg ctccctggcg ctgtccgtct gcgagggtac tagtgagacg tgcggcttcc
     5461 gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt cccgacttag gggcggagca
     5521 ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga tccgggtgac gctgcgaacg
     5581 gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc gtttcccgga accacgccca
     5641 gagcagccgc gtccctgcgc aaacccaggg ctgccttgga aaaggcgcaa ccccaacccc
     5701 ctcgagccgc ggccaaagtg gatctctgct gtccctgtaa taaacccgaa aattttgaat
     5761 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     5821 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     5881 tgccatttgt ctcgaggtcg agaattctta ttacttgtca tcgtcatcct tgtaatcaat
     5941 gtcatgatct ttataatcac cgtcatggtc tttgtagtcg cctgcggccg cgctgcctcc
     6001 gccctcctct tcttcttcct cttcctcctc aaaatgagcc tgcttcttgc gaacgttcca
     6061 atgaagacat tgagccaggg attcaaacca atctgatacc gggtcgcgca cacaaatgct
     6121 cggcagagga tagcaggaag tggtgatact tatggaatcc ccgtgccgga tttcctgccg
     6181 ttttctccca tcgaaggaca cccaagcggt attccgcgct tcaggggaca gcataatttt
     6241 caactctact ccggccggta ctactatcgg tcggaaagag agtgagtgag gacaaatcgg
     6301 tgtgatcatt atggccggca cgtttgggtg gatcattgac gcacctgcag cggctgcata
     6361 ggctgtggac ccggtgggtg tgctcacgat aaccccatca ccttggaccg tggtaatcaa
     6421 atgaccgtcc agatatacgt ctacgttact gaggtaggaa ctcgggccgc ggtcgataac
     6481 aacttcgttc aggacttgat attgcatagc ctgtttgcct acatccatgt ccaggccggc
     6541 tgcttgactc ccgttttctc cgagaccgtt atggacggct gttttcttcc ctcgcagttc
     6601 ctttacaacc ctgactttca ggcgggagcg caaaacaacg gcggcgttac cctctatgac
     6661 ctgcgttacc tgagattgaa agttctcaaa agagaatggc gtgagaaaac caagagagcc
     6721 gagatggaat gccatgacag gtggaacgct gccctgaaaa agggaggatg catacagaag
     6781 cgtcccggcc cctcccaaac agattataaa gtcgatctga ttggatatgt cgtcgtaatc
     6841 ttcccgaaac gtacaaaatt tttttttcac agcgccaaaa ctttcatcag aagcgatcgc
     6901 cgggtcctcg agtactttct tttcgacgta aacgatcata ttttcttcca tcaagtgtgt
     6961 gcaaagctct ttgaacggtt ggaggagtga cgcatcccgc atttttttga ttacgagaac
     7021 ggactttgga ctcttattcc acgtgagcct ctgggaagct ggatcctgaa tgtgcatgat
     7081 tgtctgcgga ttctgaagaa cacatgcctt gggcccaaat gtggtcacag gacaaggccc
     7141 gtggagactt ctagttcggc ggaattcctt agtagacccg agggctgggg acgcagacaa
     7201 gcttctgctc tttgctctgc ctcgtatagg atggttatag ctccatgttt cgtcaccatg
     7261 gcacgctgaa cagcaatatg cggcagcgtc tgggctaagc tctttattca tcgtcatttt
     7321 ctcctgctcc atctccattg tcaggttaat taaaccggta gcgctagcgg atctgacggt
     7381 tcactaaacc agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     7441 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
     7501 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     7561 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
     7621 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     7681 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatag tccattttaa
     7741 aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt
     7801 gtactaatat ctttgtgttt acagtcaaat taattccaat tatctctcta acagccttgt
     7861 atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctcggtgaag ggggctgctg
     7921 ctcgaggcta gtctcgtgat cgataccgtc gagatccgtt cactaatcga atggatctgt
     7981 ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg
     8041 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
     8101 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
     8161 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta
     8221 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
     8281 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
     8341 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa
     8401 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca
     8461 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct
     8521 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt
     8581 gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca
     8641 agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc
     8701 tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata
     8761 attcacttct ccaatt
//
