LOCUS       sequence-465958-        8776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector for NADK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Flickinger KM, Mellado Fritz CA, Huggler KS, Wade GM, Chang GR, Fox 
            KC, Simcox JA, Cantor JR
  TITLE     Cytosolic NADK is conditionally essential for folate-dependent 
            nucleotide synthesis.
  JOURNAL   Nat Metab. 2025 May 2. doi: 10.1038/s42255-025-01272-3.
  PUBMED    40316835
REFERENCE   2  (bases 1 to 8776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2025 May 2. doi: 10.1038/s42255-025-01272-3."
COMMENT     Sequence Label: pLJC2-NADK-3xFLAG
FEATURES             Location/Qualifiers
     source          1..8776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     266..286
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             497..730
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    802..923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(839..858)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     893..912
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      963..1098
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1118..1137)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1119..1137)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1147..1164)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1147..1163)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1156..1178)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1305..1760
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1392..1411)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1602..1623
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1786..1890
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1891..1959,1960..2751)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2109..2128)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2922..3510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3411..3430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3664..3681
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3798..3819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3834..3839,3840..3857,3858..3864)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3872..3888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3877..3899
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3896..3912
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3896..3912
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3931..3951
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3933..3951
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3979..4205
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4206..4386
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4433..4558
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5051..5284
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5469..5513
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     5811..5831
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        6120..6323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6274..6294
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             7735..7800
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7848..7965
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8014..8524
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8443..8461
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(8546..8776,1..369)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8546..8565)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
       61 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
      121 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
      181 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
      241 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
      301 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
      361 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
      421 gcatcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
      481 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
      541 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      601 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
      661 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
      721 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
      781 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
      841 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
      901 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
      961 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     1021 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     1081 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     1141 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     1201 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     1261 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     1321 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     1381 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     1441 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     1501 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     1561 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     1621 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     1681 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     1741 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1801 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1861 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1921 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1981 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2041 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2101 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     2161 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2221 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2281 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2341 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2401 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     2461 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2521 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2581 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2641 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2701 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2761 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2821 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2881 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2941 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3001 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3061 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3121 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3181 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3241 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3301 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3361 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3421 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3481 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3541 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3601 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3661 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3721 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3781 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     3841 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     3901 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     3961 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     4021 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     4081 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     4141 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     4201 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4261 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4321 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4381 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     4441 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     4501 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     4561 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     4621 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     4681 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     4741 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     4801 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     4861 agagcaaaac aaaagtaaga ccaccgcaca gcaagcagca gctgatcttc agacctggag
     4921 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     4981 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     5041 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     5101 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     5161 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     5221 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     5281 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     5341 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     5401 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     5461 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     5521 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     5581 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     5641 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     5701 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     5761 ctcgacggta tcgatcacga gactagcctc gagcagcagc ccccttcacc gagggcctat
     5821 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
     5881 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
     5941 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta
     6001 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     6061 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     6121 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     6181 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     6241 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     6301 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt
     6361 ttaattaacc tgacaatgga gatggagcag gagaaaatga cgatgaataa agagcttagc
     6421 ccagacgctg ccgcatattg ctgttcagcg tgccatggtg acgaaacatg gagctataac
     6481 catcctatac gaggcagagc aaagagcaga agcttgtctg cgtccccagc cctcgggtct
     6541 actaaggaat tccgccgaac tagaagtctc cacgggcctt gtcctgtgac cacatttggg
     6601 cccaaggcat gtgttcttca gaatccgcag acaatcatgc acattcagga tccagcttcc
     6661 cagaggctca cgtggaataa gagtccaaag tccgttctcg taatcaaaaa aatgcgggat
     6721 gcgtcactcc tccaaccgtt caaagagctt tgcacacact tgatggaaga aaatatgatc
     6781 gtttacgtcg aaaagaaagt actcgaggac ccggcgatcg cttctgatga aagttttggc
     6841 gctgtgaaaa aaaaattttg tacgtttcgg gaagattacg acgacatatc caatcagatc
     6901 gactttataa tctgtttggg aggggacggg acgcttctgt atgcatcctc cctttttcag
     6961 ggcagcgttc cacctgtcat ggcattccat ctcggctctc ttggttttct cacgccattc
     7021 tcttttgaga actttcaatc tcaggtaacg caggtcatag agggtaacgc cgccgttgtt
     7081 ttgcgctccc gcctgaaagt cagggttgta aaggaactgc gagggaagaa aacagccgtc
     7141 cataacggtc tcggagaaaa cgggagtcaa gcagccggcc tggacatgga tgtaggcaaa
     7201 caggctatgc aatatcaagt cctgaacgaa gttgttatcg accgcggccc gagttcctac
     7261 ctcagtaacg tagacgtata tctggacggt catttgatta ccacggtcca aggtgatggg
     7321 gttatcgtga gcacacccac cgggtccaca gcctatgcag ccgctgcagg tgcgtcaatg
     7381 atccacccaa acgtgccggc cataatgatc acaccgattt gtcctcactc actctctttc
     7441 cgaccgatag tagtaccggc cggagtagag ttgaaaatta tgctgtcccc tgaagcgcgg
     7501 aataccgctt gggtgtcctt cgatgggaga aaacggcagg aaatccggca cggggattcc
     7561 ataagtatca ccacttcctg ctatcctctg ccgagcattt gtgtgcgcga cccggtatca
     7621 gattggtttg aatccctggc tcaatgtctt cattggaacg ttcgcaagaa gcaggctcat
     7681 tttgaggagg aagaggaaga agaagaggag ggcggaggca gcgcggccgc aggcgactac
     7741 aaagaccatg acggtgatta taaagatcat gacattgatt acaaggatga cgatgacaag
     7801 taataagaat tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa
     7861 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     7921 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     7981 gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc ttttccaagg
     8041 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
     8101 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
     8161 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
     8221 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
     8281 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
     8341 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
     8401 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     8461 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     8521 ccagggggat ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     8581 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     8641 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     8701 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     8761 ggtggcggtc tggacc
//
