LOCUS       sequence-465968-       20432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate 
            inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for 
            protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20432)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)
FEATURES             Location/Qualifiers
     source          1..20432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1497..2108)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2512..2539
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             5491..5508
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      14056..14083
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14215..14242
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14674..15525)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15512..16351)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(16862..17833)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19072..19159
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19501..19895
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20058..20101)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20197..20306
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaatggg taaactgcat atgtcgcgac gggatttcgt caaagtctcg
     2641 gtcgtgacag gcgcggcgtt cggcatctcc gcgttggctg agccgagttt ggctgttgct
     2701 gacgatgccg tcgataagcc ggtgggaggt gacatcaagc gtatccgaac agggtgtcgc
     2761 ggctgcggca aaatggaatg cggcgttatc gccgtagtgc agaacgggcg tgtcgttcgg
     2821 atggagggcg accctacggc gttccagtcg atggggaact gctgcaccaa atcgcaagcg
     2881 gccattcagg cggcatacca tcctgatcgt ctgcattatc ctatgaaacg aacgaacgac
     2941 aaggaatccg atgatcccgg ttgggtgcgt atttcttggg atgaggccat gaccacgatc
     3001 gtgtcgaaat tcgacgagct gcaagcgcgc tacggaggcg aatcgctgtt cggcatgtgc
     3061 ggcacgtcgc gcgtgtggtg catgttcgga gcgtccaacg gcatgtacct gtgggattcg
     3121 ccgaacatcg tgcaggcgtg gcagatctgc aagggcccgc gccatttcgc cactcttatg
     3181 tgctcgagct tcgccgacag ctggatggag acggttgccc atcctgatgt gtacgtggcc
     3241 tggggcggtg catccgagat gtcgaactac gatgacagct gccgcaccac tgtggatgtg
     3301 gcgacgcgct cgaccacgca catttgcgtc gatccgcgcg aaacgaacat gggtaaagag
     3361 gcggacttcc agctgcacct gcgacccggt accgatgggg cgatggcgtt ggcttggacg
     3421 aacgtcatca ttgaaaacaa tctgtacgac gagttgttca cgaagaagtg gaccaacggt
     3481 ccgtttctcg tgtgcgaaga catggagccg accggctgga ccgaggcttc gcccattgtc
     3541 atgaccccat tcgaactcaa gacgcatttg ctgaaggaat cggatctgct ggaggacgga
     3601 agcccaaagc gttttatggt ttgggacaac cttgctggga ggctgaccta tttcgattcc
     3661 gaaaccggtt tttgggaagg tgaggaatgg acgaagccga cggccgggaa ggaagcttcg
     3721 caggagaatc tcgcgcctgg cttccatcaa ggttttgtca ttgatcctac tccgttcgac
     3781 cctgaaatag atccggcgtt gtttggggaa ttcgaagtga cgctcaaaga cgggtcgacg
     3841 cataaggtgc agccggtgtt tcaaaagtac gccgatcgct gcgcggaata cactcctgaa
     3901 gtcgctgagg aaatcaccgg ggttccggcc gatcagattc gtgcggcggc tatggcttac
     3961 ggcacgcccc ttcatccgga aaagggttat ggcaatggcg gtattcagta catgctggcc
     4021 accgagcatg cgaataccgc catccagaat gtgcgcgcgc ttgattatct gacggccatc
     4081 acgggaaatt acgatacgcc gggcggtcag cgtgcgtcaa cgcgcgctcc catcgagggc
     4141 ggtcagatgg gttttgccaa caacggttcg ggcatgccca tgctttcgcc ggggcagatg
     4201 gagaagatct taggtcgcga tgagattccg ctgctgtatt ggtggggcat gtgggccgat
     4261 gctgcggcta cgtggaatgc ggtgctcacc ggagaaccgt atccggtcgt tggcgcgttc
     4321 aattcgtcgg ggaacttcat gaaccagtgc aacaccacca cgacatggga ggctctcaag
     4381 aagctcgatt tttatgtgga atgcaacctg tggcacactc ctggcggtgg tttgtgcgac
     4441 atcgttttgc ctgccgctca tttcttggag ttgtccagtc cgcgttcatc ccagggcgct
     4501 tcgggcgcta tgggcgcgac ggtcaaatgc atcgacccgc ctggtgaagc gaagttcgac
     4561 ggcgagatca ttcgcatgtt gtacgaatac aaaggcctcc cctataacgt tattcctggt
     4621 tatcctgaat atcctagcgt cgaggaaatg ctggacatgt cggtggcggg tttcgagccg
     4681 aagggatgga aggtattcga gcagcgtttc caggaacatg gctggtggga ttgcaaggaa
     4741 actgaaccgg aattgtgggg aacgtatcgc cgctacgaaa ccggtgcgaa tcgcgctcgc
     4801 aatgcgggtg ggttcgtagg ttctcaaggt gattggcttc ccgggttcta cactccgaca
     4861 aagaagatcg aaatatggtc tacggtcatg gagtcgttcc atcccgatgg cgagtggaat
     4921 cttcccacgt atcgcgaacc accgcacagt cctgtgagcg atcctgacat gtacaaagaa
     4981 tacccgctga tcatcactac gggacgccgc attcccgtgt acttccattc cgagcaccgg
     5041 cagctgccgt ggtgtcgcga gcaatggccg gtgccgcgcg tggaaatcaa tccagacgat
     5101 gccgccacgt atggcataga acagggagat tgggtttgga ttgagacgcc atatgggaag
     5161 attcgcgagg ttgccgattt gtactacggg atccgtcccg gcactatcaa ttgcgagcat
     5221 cagtggtggt tcccggaatt caagcaatcg ggatcgggcc acgagctatg cgcggtcaac
     5281 cacttgatag acaactgggc gcgcgatccg cattgcggcg cgtctaacct gcgtgcgtac
     5341 ctcggcaaga tatacaaggc aacgcccgag aactccccgt tcggcaatcc ggtgccttgc
     5401 gaccacaacg gcgttgagat catccacgat gcttccgatc ctcgtctcaa agaatggctg
     5461 ccgacgtacg aagggaggga gggcagcagc catcaccatc accatcacta ggccatgacc
     5521 cagtatgcta ttgtcaccga cttgaatcgg tgcgtcgggt gcttggcatg cgctgtcgca
     5581 tgcaaagccg tgaacgatgt gcccattggc agtttttgga acaaggtttt acgcatcggg
     5641 ccgaatgctt ctatacaagg tgcgcaggat gcggattctt atctttactt cctgcccgtg
     5701 cagtgccagc actgcgccga ccccgagtgc gtgaaggtgt gcccgacggg cgcctcgcac
     5761 aagcttgagg acggcacggt gcaggttgac aagtccaagt gcatcggctg ccagttctgc
     5821 gcgatggctt gtccctacaa cgttcgttac ttgaacgagc aggagcgcgt ggttgagaag
     5881 tgcacccttt gcgagcagaa gatcgcccag ggcgagctgc cgcaatgcgt cagccaatgc
     5941 ggtggcatgg cgcgcttctt cggtgatttg gataagggca tcgaatcgtt cgaaggtgcg
     6001 gaaaacggaa ccgagcgcat tgttctcggg gagttctgca agcccttcac ggatgaagac
     6061 gtgcacgtgc tgcctaatgt gggaaacaat ccgtcggtgc gctatatcct gcgtgaccat
     6121 gaatggcaag gaggcggtga ataatggagc tccagtggcc tttgatcgtg ttcaccacgc
     6181 tggtggcgtg gagcgcgggg ctgttcggga cgcaggcgct gatggccgcg ttcggcgtcg
     6241 ggaagaaggc gcaggtcccg gcctggatcg cgagcgccgt ccttctggcc gtcggcggga
     6301 tcgcggtgtt cttccacctc gagcactggg agcgcatctt caacggcttc ggccacctga
     6361 cgagcggcat cacccaggag ctgatcgcca tcgtcgtgct ggcggccgtc gccgtggcct
     6421 acctggtcat gctgcgcaag tccgacgacg gcgcgagcgt gccgaagtgg ctggcgtggc
     6481 tgtcggtcgc gctgtcggtc gcgctggtgg ccgtgatggc gcactcgtac acgatggccg
     6541 cgaggccggc gtgggatagc gtgctgtgga tcctctacgt cctgggcaac gcctgcgtgc
     6601 tgggaccggc gacgttcgcg ttcctcatgg ccgcagcgcc gcccgcaggt cagcccgccg
     6661 ggcgcgcggc cgacgccggg gctcccgcag gcctcgctgc cctcgtaggc gccgccgcga
     6721 acgccctggc cgccctcgcc ttcgcgatct tcctccagct ctccgccggc tccttcgccg
     6781 acgtcggcta ctacttcgac ccgacgcatc cgaccgaggc catggccgac gccgcggcga
     6841 cggtcggcgc gcaagcgccc ctgctgtggc tcggcgcggt cgccgtcggc gctatcgtcc
     6901 cgctcgcggc ggccttcatg ggcaggcgct ccggcaactg gaggctctgg gccccggtgg
     6961 ctatcatcgc cgccctggcg ggcgccgtct gcatgcgcgt ggccttctac aacctcggcc
     7021 tcagcgtgtt catgttctac tagactagtc cgctggttgc cacgacgccc tcgaagtctc
     7081 cttcgagggc gttctgtcgt ttgcaagaag ggtctggaga ggcaaatcgc gctcgaaggg
     7141 gcttcgaggc cggggtaggt cgggagggtg gaatccggat gtttcacgtg aaacatccgt
     7201 tcttttacgg ccggtgtttc gcgtgaaaca ttttgcggaa aggactcctt cccttcccgt
     7261 gcgccacccc aacgccccgc tcctagcgct cgtacacctg gtcgcagttg agcagatcct
     7321 tcatggagtg ccagatctgg tgcgggttgc ccggcgggtt cttgagcggc ttcatcggat
     7381 agcgctcctg cgcgcccgac agcagatcga cggcgaacac ttcgtcggac agctcgagcg
     7441 cgccttccgg gcagatgtcg gtgcagcagc ggcacttcac gcaatcgacc ggacgatgcg
     7501 tcacgccgat cgagccgtcc tcacccgcgt acttcgcgat ggccccggtg gggcagaacg
     7561 tggcgcacat ctggcacgac gagcacttct cgggatcgat gatcacatgg ccccacaagc
     7621 gcgtgtccat gagcacatcc tgcggctgcc ccaacgaatc gagcgcggcc agcaaacgtt
     7681 cgcggcggtc gggaatgaag tgaggcagcg tgccgtcctt gcccaccttc acgaacctcg
     7741 gctcccgccg ctcctccaca cccaatgcgt ccttgacggc gtagtcagcc gtcaccgccg
     7801 caacgttctt agcctcgtcc ttcatgctgg agaagaagct ccgacggctc gcgtcgtagc
     7861 cctcgtcgcc cgcccggcgc acggatgagg gaagcttttc cgccaggtcg atgcgcatat
     7921 cgttgttcca ggttgcaagc agcgcgcttg ccgtgtcgca cacaacccgc gccatctcga
     7981 aacccgagac atgctcgcat tgcgcgcagc gcgcctgcac gaggctcacg cggcgcacgc
     8041 ccaacgccgc cagcgtgacc agcaggctct cctccacgcg ccccaggcac gtgacgccca
     8101 ccaccttctc ggggtcgtac aacccgtcgg ccgcggcaag cagctgatcg caggcgataa
     8161 ccacctgtcc gtcggccacg tcagccgccg cgcggcatgc ttgcagaagc tcggcatcgt
     8221 tcggacgatg cgcttcaagc gcgcaggtgg gacacacggt ggcgcaggtt ccgcacccga
     8281 tgcaccgctc gggttcgatg accagctcgt tgtcgtcata ggagatgcaa ccgctcgtgc
     8341 acacgtcggc gcacttcatg cacgttgcgt tgcgattgcg cacaatagcg caacgaccct
     8401 gatgaaccgt aatgtcggcg ctctgaagtt gttcgagcag accgaggaaa ttcgtcacgt
     8461 gagccatgac ttcacccttt ctcgtctacc cccttgcacc gcacagtata accactttcg
     8521 ccgtctcacg cgagaaaaaa cgcgcttgac accttcgaca ggcctcccta tcatgtggcc
     8581 ttggggagtc cgccggcacg ctgatcgcgt gcgaagcgga cattgtttgc aaaagtggga
     8641 acctcagggg tgaggtttcg cggtgctgtg cgccgtaagt gcaaacaatg tctgacgagg
     8701 ggcggaacat gactcacgat tccacgaacc agcatctttc tcttgagccc gactacgacg
     8761 ggttcgatcc acttgaagat ttcgaagacg atgcgaccga ggtcgaaggc cgtgccgacg
     8821 agttcgtcgc cacggtgagc gccgccgaag acgcgctcgc tcacgacgcg atggcacccg
     8881 ccacgcccgc catctgcgaa aagcccgctg aagagcgcat cgccgacttg ttggaggcca
     8941 tggcgccgcg ccgcacggtg ctgctgggca tcctggccgc ctgcgccgag ccgcagccgg
     9001 catccgccgt gaacgctcgc gtcgacgagc tgcaggagaa caacttctcg gtgtacagcg
     9061 cggccaacct gtgctccttg ctggagaagg cgggcgccat cgagcgcgtc acggccgagg
     9121 gcgaacccgc ggaggacatc gaaatcgagc cacagaccgt cgtcgtcgac ggcgtcgaat
     9181 acctggaagc ccgcgaaccc gtcgagatct actggcacat caccgagccg ggccgcgcgg
     9241 cgcttgaggc cgacaaaccg ctcgagcgcc tgcgcgccct cctggacgaa gatgccgcct
     9301 acgcgcccat ctaccagcgc atcctccgct tgtgcacagc agacggcggc gccacgacac
     9361 ccgccatcaa caacgcggtc gaccacaacc cgctggtgca gaagccgcgc ttctacgcgc
     9421 cccacttcgt cgaccgcctg gagaaatgcg acgcgctggc ctggaagaag gcgtggtgca
     9481 tcaccgacat cggacgcgcc ggcctcgata tgctggcaga cgtcgtagac gagaacgcgc
     9541 ccgccaccca gcccgaaacc cccgcaaccc ctgatcccgc cgcatccaag gaggactaac
     9601 catgaccgaa gcaaccgcgc ccgcaaccga gaccctcgcc gacgaagcga ccatgcagga
     9661 gctcgtcaaa ctgaacgagc agcgcgccgc cacgtacggc ctgctgtcgc gcctgtaccg
     9721 cgtggaggtg gaccagcccc ttctcgacga gctgcgcagc atgcgcttcc ccgccaagac
     9781 gggcaacgag aacgtggacg agggctaccg cctgctggcc acgtacctga gcaacacctg
     9841 ggacaacagc gtcaccgacc tcgccatcga ctacgtgcgc gtgttcatcg gacacggcat
     9901 cgacgcgttc tcggccgcct acccgttcga atcggtgtac acctccgaga agcgcctgct
     9961 catgcaggaa gcacgcgacg aggtgctggc catctaccgc agcgcaggcc tcgacaagca
    10021 ggacacatgg aaagagggcg aggatcatat cgcgctcgag ctggaattcg agcagatcat
    10081 ggccacgcgc acggtcgaag cgctgcgcaa aggcgacgaa gacgaggccg tcgcgctgct
    10141 gaccacgcag aagaactttc tcgaagacca tctggcggca tgggctccca tgatgacctc
    10201 cgacatgaag cgattcgcgc aaaccgactt ctaccggggc ttggcgtact tgacggacgg
    10261 gttcctccaa accgaccaaa cgttcctcga cgacgtgctg gccgaagact aactgcacag
    10321 gatcgaaaga aggagcgatg gacaacaagc aggagaaaac ctacgccggc atgacgcgac
    10381 gcacgctgtg cctgggcgca ggcggcgctg cggtcatgct aggtctaggc ggattgaagt
    10441 acgcgggatc gcaaccgctc gtgcgcccgc cgggaggcca ggacgaggaa cggctcatct
    10501 cggcctgcat ccgctgcgag aagtgctacg agatctgccc gcgcgacgtc atcgcgcccg
    10561 cccacctcga ggacggtgtg ctgaacatgc gcacccccac gttcgacttc tcggccaact
    10621 ggtgcgactg gtgcgccgaa gagcacgacg gcaccccgtt gtgcgtgagc gcctgcccca
    10681 ccgaagcgct caagctgccc gacgacgcca cggccgagaa caccatcatc ggcaaagccg
    10741 ttatcaacac ggactggtgc ttggcgtaca agctgatcgg atgccggttt tgctacgacg
    10801 cctgtcccta cgaggccatg gagctcgacg agaacaaccg ccccgtcgtg atcgaggaca
    10861 agtgcaacgg ctgcggcgcg tgcgagagcg tgtgcgtctc gctgcagaac ggctccatct
    10921 ccgaaggagc caccgcgcgc gccatcgtcg tccacccgct tgacgaagtt gaaggataag
    10981 gtgccctcat gaagaagaac tccaagaagc tgcgcaccct cgtcgcactg gccatcgtgg
    11041 ccgtcgtcgc cgtcggcttc gtcacgaacg tcgggatcgg caccatgtcg gcaccgggca
    11101 tctgggacat ctccatccta tgcccgctgg gcgccttggg aacgatgctc gcctccaaga
    11161 tgatggtgcc gcgcgccgta atctcgctga tcgtgatggt gctgctcatc gttctgttcg
    11221 cccgcgcgtt ctgcggatgg atttgccccg tgccgctcgt gcagaagttg cgcggcttgt
    11281 tcgcgaagaa gcaacccgtc gaacgcggcg acgatgccga tcctgccgcg gcgccgctca
    11341 cgaaagtcga gatcgacgcg ctgggcgttt catgctcgaa ggggtcgaag aatggcagcg
    11401 gatgcgcgtc ctgcgcctcc aagcgcggag ccgcgctcga tgctcgccac ttcgtgctgg
    11461 gcggcgcgct ggcgtccacc ttcatctttg gattcccggt gttctgcctc gtctgcccca
    11521 tcggactcac gttcgccacc atcctgctgg tcatcaacct gttctcacac ggcgatgtca
    11581 cctggtcgct catcgtggtg cccgcgctgc ttatcgccga ggtgctgctg ttcaagaagt
    11641 ggtgccataa gctgtgcccg ctttcggcgt tcatgagcct gatcgcgaag gcgaacaaga
    11701 ccttcgtgcc cacgatcgac gatgcgaagt gcctggaaac ggcgaagggc ggcgcgtgcg
    11761 gggcctgcgg ccgcgtctgc gaggagggca tcgacccgcg ccacccgcag ctttcgcagg
    11821 cggcatggag cgagtgcacc aagtgccgcg cctgcgtgga cgcatgccct gcgaacgcga
    11881 tcaccatgcc gctgctgccg cggcgaacgc cgtctaccgc agaacctgtc gccgtgccaa
    11941 cgaacgccga gaagatcgac gcggagaatg cgcgccccgt tccgtaaagc ccctcctgcg
    12001 cgacccggct cgcagctgca gcggtctggt cgcgcatccg gaatcgggcc gagcccgacc
    12061 ccgggcaaaa ggcggcttgg caagggcttt gggggacgag ccgccttttg cccgggagcg
    12121 acatccccgc tccccctccc tgcgaaaaga gggatgccgc ttccggcctt catctgacaa
    12181 ccacgacgtg ggaggacgaa aacccatggc atacgaaacg acaagcgaat cgaccgaggc
    12241 cctgctcgtc accggaggcg tcggcaccgg caaaacgcaa cgtctcgtcg agcgcgctgc
    12301 cgcgctgctg gccgaaaacg ccgccgcggg caacgtgctc gtgctgtgcg cgacgcccca
    12361 ggccgcacgc ctgttctcgg agcggttgac agcccgcgtc ggtgccaagc gcgctgccgg
    12421 cgcaaccgtc gccaccgcac gcgccttagc gctcgacgtg ctggccgacg acggcgcgcg
    12481 ccgctggtcg gggcgcgagc cgcgactact gaccgccttc gaagagacgt tcctcatgga
    12541 ggacatgaag gtgagcgggc tgcggccgaa gcggctgcgc gagatgctca agttcttcta
    12601 ccgctcgtgg accgagctgg cggacgacga tcccgattgg ctgctaccgg gcgaggaggc
    12661 cgatgtccat acgctgttga aagagaacct ggtcttcatg cgcgccgtca tagaacccga
    12721 agcggcgaac cttgctgcac gctacctccg agaacacgac gaagcgcggg cggcgcattc
    12781 ctacgcgcac gtgctgatgg acgactacgc ctgcgcaagc cgtgcctcgc agacgctggc
    12841 gaatctcgtc gcaacctgct cgatcaccgt ggcgggcgac gcggaaacct gcatcgagac
    12901 atacgattcg tacccctatg ccgccggaat cgacgaattc ctcgcaacgc atcccgatgc
    12961 tgcttgcgag cacctggatg ccgacgcgcc cgatgccgtc gaacgggtcg ctctggcaag
    13021 cgacacgccc gaagccgagt tcgaacaggt ggcgcgcacg gtggccgaag ccgtcgccgc
    13081 aggaacgccc gcacggcaga tcgtcgtggc cgtaccgaac agcgtgtggt cgcgcaacat
    13141 cgcaaccgct ttgcacgcac gaggcgtgcc aaccgaagcg ctcgccatct cgcagcccct
    13201 gcgcggcgac gtgcgggaca acgcccgctg cacgcctgcg cgcatcgcca cggccctcaa
    13261 cctcgtggcg aacccagacg acaccgcggc ttggcggtgc tggtgtggat tcggcgacta
    13321 cctggtgaac agcgcggctt tcgccagcct gcggacttgc gcgaaggagc gcagtatcgg
    13381 cctcgtggac gctttggaga tgctaagcga aaacgactgc gagcacgtag tgggcgcgca
    13441 acgcgtggcg accgcctaca aggacggtcg cgcgctcatc gagcaggtac gaggattgcg
    13501 gggcacggca ctgctggacg agctgacgcg catcgtcacc gacggagcgg aatcccaagc
    13561 gcccgccgtt ctgcgcgagc tctgcctcga gcacggcaac gcagccgatg aaaacccgga
    13621 aagcctttac gatgccgatg ctgcggcgat ggcgcggcgc atggccgagc gcctgctcgc
    13681 gcccacgctg gaggttgccg acgcggtagc cgtcgtcccc tacgacttcg cctgcggctt
    13741 ctcgcccgac ctgctgatcg tcgccggctt cgtgaacggt ttcatcccct cccgcgacta
    13801 tttcgacgcc acggtgaccc cgcccgagcg ccaggagaag atgcgcgccg tcgacgaacg
    13861 acgcgtgcgc gcgctcacag gaaaggccgg ccgcgcggtg gcgctgtcgt acttcacgtc
    13921 caccgacctc gaaagcgccg gcaagctgaa gctcaagatc agtcgcatcc gcctcaagaa
    13981 cggtatccgc acctgcacta tagagcccag cctattcctg gagcaaatcg cagcgcagga
    14041 gtagcgactc acactggctc accttcgggt gggcctttct gcgtttatat actagagaga
    14101 gaatataaaa agccagatta ttaatccggc ttttttatta tttaattcct gttataaaaa
    14161 aaggatcaat tttgaactct ctcccaaagt tgatccctta acgatccctg caggagaagg
    14221 ccatcctgac ggatggcctt tttgaagctt tctagaatcg ggggcccccg gggggattct
    14281 agaacaatgg aagtcctcgt ctcgccctcg aattagcccg cttaatgagc gggctttttt
    14341 tgaattaatt ctcgcgagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    14401 caacgcaatt aatgtaagtt agcgcgaatt gcaagctggc cgacgcgctg ggctacgtct
    14461 tgctggcgtt cgggagcaga agagcataca tctggaagca aagccaggaa agcggcctat
    14521 ggagctgtgc ggcagcgctc agtaggcaat ttttcaaaat attgttaagc cttttctgag
    14581 catggtattt ttcatggtat taccaattag caggaaaata agccattgaa tataaaagat
    14641 aaaaatgtct tgtttacaat agagtggggg gggtcagcct gccgccttgg gccgggtgat
    14701 gtcgtacttg cccgccgcga actcggttac cgtccagccc agcgcgacca gctccggcaa
    14761 cgcctcgcgc acccgctggc ggcgcttgcg catggtcgaa ccactggcct ctgacggcca
    14821 gacatagccg cacaaggtat ctatggaagc cttgccggtt ttgccggggt cgatccagcc
    14881 acacagccgc tggtgcagca ggcgggcggt ttcgctgtcc agcgcccgca cctcgtccat
    14941 gctgatgcgc acatgctggc cgccacccat gacggcctgc gcgatcaagg ggttcagggc
    15001 cacgtacagg cgcccgtccg cctcgtcgct ggcgtactcc gacagcagcc gaaacccctg
    15061 ccgcttgcgg ccattctggg cgatgatgga taccttccaa aggcgctcga tgcagtcctg
    15121 tatgtgcttg agcgccccac cactatcgac ctctgccccg atttcctttg ccagcgcccg
    15181 atagctacct ttgaccacca tggcatcagc ggtgacggcc tcccacttgg gttccaggaa
    15241 cagccggagc tgccgtccgc cttcggtctt gggttccggg ccaagcacta ggccattagg
    15301 cccagccatg gccaccagcc cttgcaggat gcgcagatca tcagcgccca gcggctccgg
    15361 gccgctgaac tcgatccgct tgccgtcgcc gtagtcatac gtcacgtcca gcttgctgcg
    15421 cttgcgctcg ccccgcttga gggcacggaa caggccgggg gccagacagt gcgccgggtc
    15481 gtgtcggacg tggctgaggc tgtgcttgtt cttaggcttc accacggggc acccccttgc
    15541 tcttgcgctg cctctccagc acggcgggct tgagcacccc gccgtcatgc cgcctgaacc
    15601 accgatcagc gaacggtgcg ccatagttgg ccttgctcac accgaagcgg acgaagaacc
    15661 ggcgctggtc gtcgtccaca ccccattcct cggcctcggc gctggtcatg ctcgacaggt
    15721 aggactgcca gcggatgtta tcgaccagta ccgagctgcc ccggctggcc tgctgctggt
    15781 cgcctgcgcc catcatggcc gcgcccttgc tggcatggtg caggaacacg atagagcacc
    15841 cggtatcggc ggcgatggcc tccatgcgac cgatgacctg ggccatgggg ccgctggcgt
    15901 tttcttcctc gatgtggaac cggcgcagcg tgtccagcac catcaggcgg cggccctcgg
    15961 cggcgcgctt gaggccgtcg aaccactccg gggccatgat gttgggcagg ctgccgatca
    16021 gcggctggat cagcaggccg tcagccacgg cttgccgttc ctcggcgctg aggtgcgccc
    16081 caagggcgtg caggcggtga tgaatggcgg tgggcgggtc ttcggcgggc aggtagatca
    16141 ccgggccggt gggcagttcg cccacctcca gcagatccgg cccgcctgca atctgtgcgg
    16201 ccagttgcag ggccagcatg gatttaccgg caccaccggg cgacaccagc gccccgaccg
    16261 taccggccac catgttgggc aaaacgtagt ccagcggtgg cggcgctgct gcgaacgcct
    16321 ccagaatatt gataggctta tgggtagcca ttgattgcct cctttgcagg cagttggtgg
    16381 ttaggcgctg gcggggtcac tacccccgcc ctgcgccgct ctgagttctt ccaggcactc
    16441 gcgcagcgcc tcgtattcgt cgtcggtcag ccagaacttg cgctgacgca tccctttggc
    16501 cttcatgcgc tcggcatatc gcgcttggcg tacagcgtca gggctggcca gcaggtcgcc
    16561 ggtctgcttg tccttttggt ctttcatatc agtcaccgag aaacttgccg gggccgaaag
    16621 gcttgtcttc gcggaacaag gacaaggtgc agccgtcaag gttaaggctg gccatatcag
    16681 cgactgaaaa gcggccagcc tcggccttgt ttgacgtata accaaagcca ccgggcaacc
    16741 aatagccctt gtcacttttg atcaggtaga ccgaccctga agcgcttttt tcgtattcca
    16801 taaaaccccc ttctgtgcgt gagtactcat agtataacag gcgtgagtac caacgcaagc
    16861 actacatgct gaaatctggc ccgcccctgt ccatgcctcg ctggcggggt gccggtgccc
    16921 gtgccagctc ggcccgcgca agctggacgc tgggcagacc catgaccttg ctgacggtgc
    16981 gctcgatgta atccgcttcg tggccgggct tgcgctctgc cagcgctggg ctggcctcgg
    17041 ccatggcctt gccgatttcc tcggcactgc ggccccggct ggccagcttc tgcgcggcga
    17101 taaagtcgca cttgctgagg tcatcaccga agcgcttgac cagcccggcc atctcgctgc
    17161 ggtactcgtc cagcgccgtg cgccggtggc ggctaagctg ccgctcggac agttcgaggc
    17221 tggccagcct gcgggccttc tcctgctgcc gctgggcctg ctcgatctgc tggccagcct
    17281 gctgcaccag cgccgggcca gcggtggcgg tcttgccctt ggattcacgc agcagcaccc
    17341 acggctgata accggcgcgg gtggtgtgct tgtccttgcg gttggtgaag cccgccaagc
    17401 ggccatagtg gcggctgtcg gcgctggccg ggtcggcgtc gtactcgctg gccagcgtcc
    17461 gggcaatctg cccccgaagt tcaccgcctg cggcgtcggc caccttgacc catgcctgat
    17521 agttcttcgg gctggtttcc actaccaggg caggctcccg gccctcggct ttcatgtcat
    17581 ccaggtcaaa ctcgctgagg tcgtccacca gcaccagacc atgccgctcc tgctcggcgg
    17641 gcctgatata cacgtcattg ccctgggcat tcatccgctt gagccatggc gtgttctgga
    17701 gcacttcggc ggctgaccat tcccggttca tcatctggcc ggtggtggcg tccctgacgc
    17761 cgatatcgaa gcgctcacag cccatggcct tgagctgtcg gcctatggcc tgcaaagtcc
    17821 tgtcgttctt catcgggcca ccaagcgcag ccagatcgag ccgtcctcgg ttgtcagtgg
    17881 cgtcaggtcg agcaagagca acgatgcgat cagcagcacc accgtaggca tcatggaagc
    17941 cagcatcacg gttagccata gcttccagtg ccacccccgc gacgcgctcc gggcgctctg
    18001 cgcggcgctg ctcacctcgg cggctacctc ccgcaactct ttggccagct ccacccatgc
    18061 cgcccctgtc tggcgctggg ctttcagcca ctccgccgcc tgcgcctcgc tggcctgctg
    18121 ggtctggctc atgacctgcc gggcttcgtc ggccagtgtc gccatgctct gggccagcgg
    18181 ttcgatctgc tccgctaact cgttgatgcc tctggatttc ttcactctgt cgattgcgtt
    18241 catggtctat tgcctcccgg tattcctgta agtcgatgat ctgggcgttg gcggtgtcga
    18301 tgttcagggc cacgtctgcc cggtcggtgc ggatgccccg gccttccatc tccaccacgt
    18361 tcggccccag gtgaacaccg ggcaggcgct cgatgccctg cgcctcaagt gttctgtggt
    18421 caatgcgggc gtcgtggcct gcccgctcta atgcccggtt ggcatggtcg gcccatgcct
    18481 cgcgggtctg ctcaagccat gccttgggct tgagcgcttc ggtcttctgt gccccgccct
    18541 tctccggggt cttgccgttg taccgcttga accactgagc ggcgggccgc tcgatgccgt
    18601 cattgatccg ctcggagatc atcaggtggc agtgcgggtt ctcgccgcca ccggcatgga
    18661 tggccagcgt atacggcagg cgctcggcac cggtcaggtg ctgggcgaac tcggacgcca
    18721 gcgccttctg ctggtcgagg gtcagctcga ccggcagggc aaattcgacc tccttgaaca
    18781 gccgcccatt ggcgcgttca tacaggtcgg cagcatccca gtagtcggcg ggccgctcga
    18841 cgaactccgg catgtgcccg gattcggcgt gcaagacttc atccatgtcg cgggcatact
    18901 tgccttcgcg ctggatgaag tcggccttgg ccctggccga ttggccgccc gacctgctgc
    18961 cggttttcgc cgtaaggtga taaatcgcca tgctgcctcg ctgttgcttt tgcttttcgg
    19021 ctccatgcaa tggccctcgg agagcgcacc gcccgaaggg tggccgttag gccagtttct
    19081 cgaagagaaa ccggtaagtg cgccctcccc tacaaagtag ggtcgggatt gccgccgctg
    19141 tgcctccatg atagcctacg agacagcaca ttaacaatgg ggtgtcaaga tggttaaggg
    19201 gagcaacaag gcggcggatc ggctggccaa gctcgaagaa caacgagcgc gaatcaatgc
    19261 cgaaattcag cgggtgcggg caagggaaca gcagcaagag cgcaagaacg aaacaaggcg
    19321 caaggtgctg gtgggggcca tgattttggc caaggtgaac agcagcgagt ggccggagga
    19381 tcggctcatg gcggcaatgg atgcgtacct tgaacgcgac cacgaccgcg ccttgttcgg
    19441 tctgccgcca cgccagaagg atgagccggg ctgaatgatc gaccgagaca ggccctgcgg
    19501 ggctgcacac gcgcccccac ccttcgggta gggggaaagg ccgctaaagc ggctaaaagc
    19561 gctccagcgt atttctgcgg ggtttggtgt ggggtttagc gggctttgcc cgcctttccc
    19621 cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag cgaatagacc agctatccgg
    19681 cctctggccg ggcatattgg gcaagggcag cagcgcccca caagggcgct gataaccgcg
    19741 cctagtggat tattcttaga taatcatgga tggatttttc caacaccccg ccagcccccg
    19801 cccctgctgg gtttgcaggt ttgggggcgt gacagttatt gcaggggttc gtgacagtta
    19861 ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca gttagtacgg gagtgacggg
    19921 cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa gaactttccg
    19981 ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg cctcatgtgg
    20041 cggccctcgc gagaattaat tcagataaaa aaaatcctta gctttcgcta aggatgattt
    20101 ctagcgatga ccctgctgat tggttcgctg accgtagact ggttggcttg gtttcatcag
    20161 ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat
    20221 tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg
    20281 gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta
    20341 cacgaaccct ttggcaaaat cctgtatatc gtgcgacatc gcgcgcgcgc gcgcggatgc
    20401 tagcggcgcg ccaaaaaaaa gcccgctcat ta
//
